Nanoproteomics
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Nanoproteomics
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Author : Steven A. Toms
language : en
Publisher: Humana Press
Release Date : 2011-09-23
Nanoproteomics written by Steven A. Toms and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-23 with Science categories.
As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues. The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular BiologyTM, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls. Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.
Nanoproteomics Enabled Strategies For The Analysis Of Cardiac Troponin
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Author : Timothy-Neil Trinidad Tiambeng
language : en
Publisher:
Release Date : 2022
Nanoproteomics Enabled Strategies For The Analysis Of Cardiac Troponin written by Timothy-Neil Trinidad Tiambeng and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.
Cardiac troponin I (cTnI) is a universally-accepted, clinical biomarker for the diagnosis of acute myocardial infarction (a.k.a., heart attack). cTnI is released into the bloodstream following cardiovascular injury where it circulates with low abundance (ng/ml) and in myriad proteoforms (e.g., phosphorylated, acetylated, oxidized, and truncated). Top-down mass spectrometry (MS)-based proteomics has emerged as a powerful technology for characterizing and quantifying proteoforms arising from post-translational modifications, alternative splicing, and sequence variations at the intact protein level. However, the large dynamic range of the blood proteome makes the MS-detection of low-abundance biomarker proteins extremely challenging. Thus, protein enrichment strategies are necessary to specifically capture and enrich low-abundance proteins from complex mixtures prior to MS-analysis. In chapter 1, an introduction is given regarding the use of top-down mass spectrometry in health and disease cardiac protein biomarkers, and the need for front-end enrichment methods. In chapter 2, I describe the development of an integrated "nanoproteomics" method coupling peptide-functionalized superparamagnetic nanoparticles (NPs) with top-down MS for the enrichment and comprehensive analysis of cTnI proteoform monomers, directly from serum for the first time. Chapter 3 utilizes the NP platform developed in chapter 2 to purify and enable the native MS analysis of intact, non-recombinant cardiac troponin complexes (cTnT-cTnI-cTnC; 77 kDa) from human heart tissues, with native MS coupled online with size exclusion chromatography (SEC), thereby enabling the native MS analysis of cardiac troponin complexes for the first time. Chapter 4 describes future directions with using nanoproteomics for the analysis of clinical acute myocardial infarction samples, and the structural analysis of cardiac troponin complexes. Finally, the thesis is concluded with two Appendices containing step-by-step protocols and methods for the analysis of cardiac troponin proteoforms by top-down mass spectrometry, and size exclusion chromatography strategies combined with MASH Explorer for targeted proteoform characterization. Overall, the work described here demonstrates how nanoproteomics can provide proteoform- and protein complex- resolved details of cardiac troponin to elucidate the molecular mechanisms underlying health and disease.
Nanogenomics And Nanoproteomics Enabling Personalized Predictive And Preventive Medicine
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Author : Nicola Luigi Bragazzi
language : en
Publisher:
Release Date : 2014
Nanogenomics And Nanoproteomics Enabling Personalized Predictive And Preventive Medicine written by Nicola Luigi Bragazzi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Defining The Molecular Landscape Of Low Abundance Protein Biomarkers By Top Down Proteomics And Nanotechnology
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Author : David Stephen Roberts
language : en
Publisher:
Release Date : 2023
Defining The Molecular Landscape Of Low Abundance Protein Biomarkers By Top Down Proteomics And Nanotechnology written by David Stephen Roberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
Top-down mass spectrometry (MS) is a disruptive technology that holds enormous potential for the comprehensive characterization of intact proteins and their proteoforms to decipher post-translational modifications (PTMs) together with sequence variations and splice isoforms to connect molecular structures with biological functions. However, many major challenges limit the application of the top-down strategy for challenging biological systems. Focusing on addressing the major challenges of the human proteome dynamic range and the molecular analysis of complex proteins with diverse PTMs, my highly interdisciplinary doctoral work has sought to develop new functionalized nanomaterials and top-down MS methodologies to significantly advance our understanding of complex and low abundance proteoforms. Chapter 1 introduces the key concept of 'proteoform' biology and provides a comprehensive review of the challenges and recent advances in top-down MS proteomics, with an emphasis on the proteome dynamic range and proteoform complexity. Chapter 2 describes the novel integration of a new class of surface silanized nanoparticles (NPs) with top-down MS, hereby referred to as a 'nanoproteomics' strategy, for enriching low-abundance phosphoproteins from complex biological mixtures. This nanoproteomics approach sets the stage to explore the complex structural landscape of low abundance protein biomarkers. Chapter 3 describes a nanoproteomics strategy to enrich cardiac troponin I (cTnI), a 'gold-standard' biomarker for cardiovascular diseases, from complex biological mixtures, including human serum. Further extending to a native protein workflow enabled the enrichment of the entire heterotrimeric cardiac troponin (cTn) complex for the structural and dynamical characterization of endogenous native protein complexes from human tissues. Chapter 4 details a new top-down MS approach for the molecular and structural elucidation of complex glycoproteins, including the SARS-CoV-2 Spike protein. Lastly, Chapter 5 describes a new analytical method for the comprehensive top-down MS characterization of angiotensin-converting enzyme 2 (ACE2) to define proteoform-resolved molecular fingerprints of diverse ACE2 PTMs. Furthermore, an anti-ACE2 peptide-functionalized NP was developed for the enrichment of endogenous ACE2 from biological mixtures and human cardiac tissues, for the first time. Overall, my doctoral work provides a foundation for new classes of materials and intact protein MS strategies to provide transformative molecular insights for the analysis of cardiovascular related proteins, glycoproteins, membrane proteins, and, by extension, the human proteome.
Quantitative Nano Proteomics For Protein Complexes Qnanopx Related To Estrogen Transcriptional Action
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Author : 鄭百喬
language : en
Publisher:
Release Date : 2010
Quantitative Nano Proteomics For Protein Complexes Qnanopx Related To Estrogen Transcriptional Action written by 鄭百喬 and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
Smart Nanomaterials Targeting Pathological Hypoxia
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Author : Sonam Chawla
language : en
Publisher: Springer Nature
Release Date : 2023-06-29
Smart Nanomaterials Targeting Pathological Hypoxia written by Sonam Chawla and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-06-29 with Science categories.
The book showcases the newest research on smart nanoplatforms responding to “hypoxia”; an omnipresent pathological feature of many modern-day diseases and its potential applications. The book demonstrates the versatility of hypoxia targeted smart nanoplatforms in disease management in diverse pathological settings presented in chapters contributed by various experts in the field. The themes in the book touch upon critical facets to address pathological hypoxia such as nanomaterials enhancing oxygenation, hypoxia responsive nanoformulations which deliver the drug directly to the hypoxic site, limited toxicity and enhanced efficacy of smart nanoformulations to counter molecular consequences of hypoxia, and theranostics combining hypoxia detection systems with drug delivery systems. This book disseminates the current implications of smart nanomaterials in disease management that can be exploited by scholars, researchers, and pharma industries to develop, manufacture, and commercialize hypoxia targeted diagnostics and therapeutics.
Bionanotechnology
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Author : Anil Kumar Anal
language : en
Publisher: CRC Press
Release Date : 2018-02-02
Bionanotechnology written by Anil Kumar Anal and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-02 with Medical categories.
This book deals with a subject of high interest and importance in all sectors, including biomedical, food, agriculture, energy, and environment. Biological systems are essential in nanotechnology, and many new applications are being developed by mimicking the natural systems. Approaching these topics from an engineering perspective, the book offers insight on the details of nanoscale fabrication processes as well as cell biology. The basics of biology and chemistry, with a focus on how to engineer the behavior of molecules at the nanoscale, are also explored and analyzed. The aim of the text is to provide the reader with broader knowledge of biological methods for signal transduction and molecular recognitions systems and how they can be replicated in bio-sensing applications. The reader will learn the basic structures and interactions of biomacromolecules for developing biocompatible and eco-friendly devices.
Pharmaceutical Biotechnology
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Author : Carlos A. Guzmán
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-01-01
Pharmaceutical Biotechnology written by Carlos A. Guzmán and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-01-01 with Medical categories.
Pharmaceutical Biotechnology is a unique compilation of reviews addressing frontiers in biologicals as a rich source for innovative medicines. This book fulfills the needs of a broad community of scientists interested in biologicals from diverse perspectives—basic research, biotechnology, protein engineering, protein delivery, medicines, pharmaceuticals and vaccinology. The diverse topics range from advanced biotechnologies aimed to introduce novel, potent engineered vaccines of unprecedented efficacy and safety for a wide scope of human diseases to natural products, small peptides and polypeptides engineered for discrete prophylaxis and therapeutic purposes. Modern biologicals promise to dramatically expand the scope of preventive medicine beyond the infectious disease arena into broad applications in immune and cancer treatment, as exemplified by anti-EGFR receptors antibodies for the treatment of breast cancer. The exponential growth in biologicals such as engineered proteins and vaccines has been boosted by unprecedented scientific breakthroughs made in the past decades culminating in an in-depth fundamental understanding of the scientific underpinnings of immune mechanisms together with knowledge of protein and peptide scaffolds that can be deliberately manipulated. This has in turn led to new strategies and processes. Deciphering the human, mammalian and numerous pathogens’ genomes provides opportunities that never before have been available—identification of discrete antigens (genomes and antigenomes) that lend themselves to considerably improved antigens and monoclonal antibodies, which with more sophisticated engineered adjuvants and agonists of pattern recognition receptors present in immune cells, deliver unprecedented safety and efficacy. Technological development such a nanobiotechnologies (dendrimers, nanobodies and fullerenes), biological particles (viral-like particles and bacterial ghosts) and innovative vectors (replication-competent attenuated, replication-incompetent recombinant and defective helper-dependent vectors) fulfill a broad range of cutting-edge research, drug discovery and delivery applications. Most recent examples of breakthrough biologicals include the human papilloma virus vaccine (HPV, prevention of women genital cancer) and the multivalent Pneumoccocal vaccines, which has virtually eradicated in some populations a most prevalent bacterial ear infection (i.e., otitis media). It is expected that in the years to come similar success will be obtained in the development of vaccines for diseases which still represent major threats for human health, such as AIDS, as well as for the generation of improved vaccines against diseases like pandemic flu for which vaccines are currently available. Furthermore, advances in comparative immunology and innate immunity revealed opportunities for innovative strategies for ever smaller biologicals and vaccines derived from species such as llama and sharks, which carry tremendous potential for innovative biologicals already in development stages in many pharmaceutical companies. Such recent discoveries and knowledge exploitations hold the promise for breakthrough biologicals, with the coming decade. Finally, this book caters to individuals not directly engaged in the pharmaceutical drug discovery process via a chapter outlining discovery, preclinical development, clinical development and translational medicine issues that are critical the drug development process. The authors and editors hope that this compilation of reviews will help readers rapidly and completely update knowledge and understanding of the frontiers in pharmaceutical biotechnologies.
Personalized Medicine
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Author :
language : en
Publisher: Academic Press
Release Date : 2016-01-28
Personalized Medicine written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-01-28 with Science categories.
The Advances in Protein Chemistry and Structural Biology series is an essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins, with each thematically organized volume guest edited by leading experts in a broad range of protein-related topics. Provides cutting-edge developments in protein chemistry and structural biology Chapters are written by authorities in their field Targeted to a wide audience of researchers, specialists, and students
Understanding Nanomedicine
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Author : Rob Burgess
language : en
Publisher: CRC Press
Release Date : 2012-06-18
Understanding Nanomedicine written by Rob Burgess and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-18 with Medical categories.
This book comprehensively covers a broad range of therapeutic and diagnostic applications of nanotechnology, providing descriptions of cutting-edge discoveries along with historical perspectives. The text focuses on nanomaterials and nanoparticles, the sectors that hold the most promise for the future of medicine. The author look at how nanotechnol