Nonlinear Laser Spectroscopy Studies Of Enzyme Interactions
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Nonlinear Laser Spectroscopy Studies Of Enzyme Interactions
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Author : Duncan G. Steel
language : en
Publisher:
Release Date : 1987
Nonlinear Laser Spectroscopy Studies Of Enzyme Interactions written by Duncan G. Steel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with categories.
The thrust of this project is to develop and apply new methodologies based on nonlinear optics to the study of biological macromolecules and membranes. In the period covered by this report we developed several biochemical experimental systems to which the spectroscopic techniques are being applied. The biological problems addressed include: a. Age related modifications in enzyme catalysis. 2. Mechanisms of control of membrane-bound transport proteins. The spectroscopic studies involved two primary sets of measurements based on backward nearly degenerate four-wave mixing and employing both time domain and frequency domain and frequency domain measurements. A CW dye laser system using cross-correlated optical fields was applied to measure relaxation rates of nonradiating states like nonphosphorescent triplet states and is being adapted for studies of biological systems. A laboratory for ultrafast time-domain measurements is being developed to study enzyme and membrane mechanisms. A system based on phase conjugate optics designed to eliminate the effects of light scattering in optical spectroscopy studies of biological membrane systems is being adapted for studies of biological systems. A laboratory for ultrafast time-domain measurements is being developed to study enzyme and membrane mechanisms. A system based on phase conjugate optics designed to eliminate the effects of light scattering in optical spectroscopy studies of biological membrane systems is being tested.
Nonlinear Laser Spectroscopy
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Author : V. S. Letokhov
language : en
Publisher: Springer
Release Date : 1977-03-01
Nonlinear Laser Spectroscopy written by V. S. Letokhov and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977-03-01 with Technology & Engineering categories.
The laser as a source of coherent optical radiation has made it possible to investigate nonlinear interaction of optical radiation with atoms and mole cules. Its availability has given rise to new research fields, such as non linear optics, laser spectroscopy, laser photochemistry, that lie at the boundary between quantum electronics and physical optics, optical spectros copy and photochemistry, respectively. The use of coherent optical radiation in each of these fields has led to the discovery of qualitatively ne\~ effects and possibilities; in particular, some rather subtle effects of interaction between highly monochromatic light and atoms and molecules, in optical spec troscopy, have formed the bases for certain methods of so-called nonlinear, laser Doppler-free spectroscopy. These methods have made it possible to in 5 6 crease the resolution of spectroscopic studies from between 10 and 10 , lim 11 ited by Doppl er 1 i ne broadeni ng up, to about 10 ; at present some 1 abor atories are developing new techniques that have even higher resolution. The discovery and elaboration of the methods of nonlinear laser spectroscopy have resulted largely from contributions by scientists from many countries, in particular from the USA (Massachusetts Institute of Technology, Stanford Uni versity, National Bureau of Standards in Boulder, Harvard University, etc. ), the USSR (P. N. Levedev Institute of Physics, Institute of Semiconductor Phys ics in Novosibirsk, Institute of Spectroscopy, etc.
Introduction To Nonlinear Laser Spectroscopy 2e
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Author : Marc Levenson
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Introduction To Nonlinear Laser Spectroscopy 2e written by Marc Levenson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Introduction to Nonlinear Laser Spectroscopy, Revised Edition presents the most useful nonlinear spectroscopy techniques at a level accessible to spectroscopists and graduate students unfamiliar with nonlinear optics. This book discusses the principles of nonlinear laser spectroscopy. Organized into seven chapters, this edition starts with an overview of the stimulated Raman effect and coherent anti-Stokes Raman spectroscopy, which can be used in a varied way to generate radiation in the ultraviolet and vacuum-ultraviolet areas. This text then explains the exciting possibilities started by saturated absorption and related techniques, including improved spectroscopic precision, studies of collisional dynamics, and better measurements of fundamental constants and of basic units. Other chapters examine Hamiltonian relaxation, which describes all of the processes that return the ensemble to thermal equilibrium. The final chapter deals with the method of infrared spectrophotography, which combines efficient detection, time resolution, and coherent infrared. Spectroscopists and graduate students will find this book extremely useful.
Nonlinear Optical Spectroscopy With Ultrafast Laser Pulses And Controlling Laser Matter Interactions
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Author : Gennady Rasskazov
language : en
Publisher:
Release Date : 2017
Nonlinear Optical Spectroscopy With Ultrafast Laser Pulses And Controlling Laser Matter Interactions written by Gennady Rasskazov and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with Electronic dissertations categories.
Reporter
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Author : University of Michigan. Division of Research Development and Administration
language : en
Publisher:
Release Date : 1987
Reporter written by University of Michigan. Division of Research Development and Administration and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Research categories.
D R D A Reporter
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Author :
language : en
Publisher: UM Libraries
Release Date : 1990
D R D A Reporter written by and has been published by UM Libraries this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Research categories.
Molecular And Laser Spectroscopy
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Author : V.P. Gupta
language : en
Publisher: Elsevier
Release Date : 2020-07-10
Molecular And Laser Spectroscopy written by V.P. Gupta and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-10 with Science categories.
Molecular and Laser Spectroscopy, Advances and Applications: Volume 2 gives students and researchers an up-to-date understanding of the fast-developing area of molecular and laser spectroscopy. This book covers basic principles and advances in several conventional as well as new and upcoming areas of molecular and laser spectroscopy, such as a wide range of applications in medical science, material science, standoff detection, defence and security, chemicals and pharmaceuticals, and environmental science. It covers the latest advancements, both in terms of techniques and applications, and highlights future projections. Editors V.P. Gupta and Yukihiro Ozaki have brought together eminent scientists in different areas of spectroscopy to develop specialized topics in conventional molecular spectroscopy (Cavity ringdown, Matrix Isolation, Intense THz, Far- and Deep- UV, Optogalvanic ), linear and nonlinear laser spectroscopy (Rayleigh & Raman Scattering), Ultrafast Time-resolved spectroscopy, and medical applications of molecular spectroscopy. and advanced material found in research articles. This new volume expands upon the topics covered in the first volume for scientists to learn the latest techniques and put them to practical use in their work. Covers several areas of spectroscopy research and expands upon topics covered in the first volume Includes exhaustive lists of research articles, reviews, and books at the end of each chapter to further learning objectives Uses illustrative examples of the varied applications to provide a practical guide to those interested in using molecular and laser spectroscopy tools in their research
Technical Reports Awareness Circular Trac
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Author :
language : en
Publisher:
Release Date : 1987-07
Technical Reports Awareness Circular Trac written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-07 with Science categories.
Laser Spectroscopy And Nonlinear Optics Of Solids
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Author : S. Radhakrishna
language : en
Publisher: Springer
Release Date : 1990
Laser Spectroscopy And Nonlinear Optics Of Solids written by S. Radhakrishna and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Laser spectroscopy categories.
Laser spectroscopy in solids has become one of the most exciting fields in solid state physics. Its importance has become particularly apparent since the advent of a number of new materials - both organic and inorganic - whose nonlinear optical properties can be used to generate laser beams over a wide spectroscopic range. In laboratories all over the world efforts are being made to produce new nonlinear optical materials which have attractive features for spectroscopic work. In this book the present status of the field is reviewed by researchers who have made significant contributions to the development of this frontier area. Is is an excellent introduction for anyone beginning research on the nonlinear optics of solids.
High Resolution Nonlinear Multi Photon Laser Spectroscopy As A Diagnostic Probe For Isotopes And Molecules In Biophysical And Geochemical Applications
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Author : Julia Ann Schafer
language : en
Publisher:
Release Date : 2004
High Resolution Nonlinear Multi Photon Laser Spectroscopy As A Diagnostic Probe For Isotopes And Molecules In Biophysical And Geochemical Applications written by Julia Ann Schafer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Laser beams categories.
Laser wave mixing is presented as a sensitive absorbance-based detection method for very low activity levels of protease enzymes. Trypsin and proteinase K enzyme activities are analyzed using casein protein covalently labeled with multiple fluorescent BODIPY dye molecules. A preliminary detection limit for trypsin is determined to be 6.34 × 10−14 M or 1.51 pg/mL. For anisotropy studies, wave mixing and fluorescence trends have inverse relationships at the onset of catalysis. Wave mixing is also investigated as a new optical method for the measurement of FRET. The relationship between the wave-mixing signal and FRET is verified using the acceptor/donor pair malachite green and erythrosin B. Resonance energy transfer between a fluorophore and a quencher molecule bound to complimentary oligonucleotide strands is studied to calculate the dye-to-dye distance on a 31-bp curved DNA fragment. The result suggests that there is a direct measurable relationship between the efficiency of resonance energy transfer and the wave-mixing signal. For the first time, wave mixing is applied to the measurement of analyte species in the inductively coupled plasma atomizer. The use of counter-propagating input beams yields sub-Doppler spectral resolution. Nonlinear optical coherence theory is used to predict and study the intensity and hyperfine profiles of atomic populations in the plasma torch. Wavelength modulation and ion line detection in the ICP are investigated for S/N enhancement. Laser wave mixing is also presented as an effective technique for kinetic temperature measurement in an atmospheric-pressure RF inductively coupled plasma using the 4s3P2 2!4p3D33 argon transition probed by a tunable 811.5-nm diode laser. Kinetic temperature measurements are made at five radial steps from the center of the torch and at four different torch heights. The kinetic temperature is determined by simultaneously measuring the line shapes of the sub-Doppler backward phase-conjugate wave-mixing signal and the Doppler-broadened forward-scattering wave-mixing signal.