Nucleic Acid Hybridization In The Study Of Cell Differentiation
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Nucleic Acid Hybridization In The Study Of Cell Differentiation
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Author : H. Ursprung
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-05
Nucleic Acid Hybridization In The Study Of Cell Differentiation written by H. Ursprung and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-05 with Science categories.
The informational content of cells is encoded in the nucleotide sequences of their DNA. The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription. In the test tube, these restrictions can be exploited for ascertaining similarities and dissimilarities of nucleic acids of varying origin by measuring the kinetics of reassociation of polynucleotides to double-stranded molecules in DNA DNA renaturation or RNA-DNA hybridization experiments, and by determining the thermal stability and other physical-chemical properties of the resulting hybrid molecules. This method has enormous potential for developmental biology. It offers a more direct approach to the ever-present question of the genetic identity of different cell types in an individual organism, and a more direct test of the hypothesis of diffe rential gene function. It offers the possibility of localizing genes on chromosomes without the use of Mendelian genetics. It is an indispensable tool in the isolation, purification, and characterization of genes. This volume brings together six articles by investigators actively working on various aspects of developmental biology who use nucleic acid hybridization as a tool in their research. Sound in theory, the method is in a honing phase as regards the technical detail. This is expressed in the hesitation with which some of the conclusions are rightly drawn.
Nucleic Acid Hybridization In The Study Of Cell Differentiation
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Author : Heinrich Ursprung
language : en
Publisher:
Release Date : 1972
Nucleic Acid Hybridization In The Study Of Cell Differentiation written by Heinrich Ursprung and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Cell differentiation categories.
The Stability Of The Differentiated State Nucleic Acid Hybridization In The Study Of Cell Differentiation
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Author : H. Ursprung
language : en
Publisher:
Release Date : 1968
The Stability Of The Differentiated State Nucleic Acid Hybridization In The Study Of Cell Differentiation written by H. Ursprung and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1968 with categories.
Gene Enzyme Systems In Drosophila
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Author : W. J. Dickinson
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Gene Enzyme Systems In Drosophila written by W. J. Dickinson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
There was a period in the history of modern biology when proteins were thought to be "gene products" in a rather direct sense. An account of their appearance and disappearance in the course of development and differentiation thus seemed an appropriate means of describing "gene regulation". When RNA was found to be the immediate product of genetic activity, the study of proteins as gene products lost some of its original attraction. Indeed, the development of the powerful method of nucleic acid hybridization aroused the hope that a large array of specific messenger-RNA molecules synthesized during cell differentiation could be individually assayed. The difficulties in the way of such ambitious projects were described in Volume 3 of this series: Nucleic Acid Hybridization in the Study of Cell Differentiation (ed. H. URSPRUNG, 1972). Enzymes are in large measure responsible for cell function. Clearly, their synthesis must be under genetic control. We are convinced that the study of enzyme behavior during development merits much attention, particularly if the work is carried out on a eukaryote that lends itself to genetic and developmental analysis. An impressive amount of genetic information is available on the insect Drosophila, and much has been learnt about its development. The giant chromosomes present in several tissues of this insect might well be useful in a continuing analysis of the appearance of specific enzymes and the transcription of the cognate genetic loci.
Somatic Cell Hybridization Studies On Genetics And Development
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Author : Richard L. Davidson
language : en
Publisher:
Release Date : 1973
Somatic Cell Hybridization Studies On Genetics And Development written by Richard L. Davidson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with Cell hybridization categories.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Immunocytochemistry And In Situ Hybridization In The Biomedical Sciences
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Author : Julian E. Beesley
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Immunocytochemistry And In Situ Hybridization In The Biomedical Sciences written by Julian E. Beesley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
Molecular Methods For Virus Detection
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Author : Danny L. Wiedbrauk
language : en
Publisher: Elsevier
Release Date : 1995-02-08
Molecular Methods For Virus Detection written by Danny L. Wiedbrauk and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-02-08 with Science categories.
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables
Cell Cycle And Cell Differentiation
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Author : J. Reinert
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Cell Cycle And Cell Differentiation written by J. Reinert and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
It is instructive to compare the response of biologists to the two themes that comprise the title of this volume. The concept of the cell cycle-in contra distinction to cell division-is a relatively recent one. Nevertheless biologists of all persuasions appreciate and readily agree on the central problems in this area. Issues ranging from mechanisms that initiate and integrate the synthesis of chro mosomal proteins and DNA during S-phase of mitosis to the manner in which assembly of microtubules and their interactions lead to the segregation of metaphase chromosomes are readily followed by botanists and zoologists, as well as by cell and molecular biologists. These problems are crisp and well-defined. The current state of "cell differentiation" stands in sharp contrast. This, one of the oldest problems in experimental biology, almost defies definition today. The difficulties arise not only from a lack of pertinent information on the regulatory mechanisms, but also from conflicting basic concepts in this field. One of the ways in which this situation might be improved would be to find a broader experimental basis, including a better understanding of the relationship between the cell cycle and cell differentiation.
Genetic Mosaics And Cell Differentiation
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Author : W. J. Gehring
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Genetic Mosaics And Cell Differentiation written by W. J. Gehring and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
The relationship of cell lineage and differentiation is one of the most intriguing problems in developmental biology. In most higher organisms, the analysis of the cell lineage has to rely on indirect methods. Only in the most suitable systems, like the nematodes, can the pattern of cell division be determined by direct observation under the microscope. In cases where this is not possible, the fate of the cells has to be examined by using cell markers. Most suitable for this purpose are genetic markers, provided that they do not interfere with the developmental pathway to be studied. However, suitable genetic markers and techniques for generating genetic mosaics are available in a few organisms only. Therefore, this volume is largely concerned with Drosophila and the mouse, which have been studied most extensively. In 1929 STURTEVANT introduced the analysis of gynandromorphs into devel opmental genetics. However, this important contribution remained largely unnoticed until the late sixties, when the potential of this technique for deter mining embryonic fate maps and the number of primordial cells was exploited, and the methodology extended to the mapping of mutational foci. Mitotic recombination was demostrated by STER:-