Parallelized Microfluidic Devices For High Throughput Nerve Regeneration Studies In Caenorhabditis Elegans
DOWNLOAD
Download Parallelized Microfluidic Devices For High Throughput Nerve Regeneration Studies In Caenorhabditis Elegans PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Parallelized Microfluidic Devices For High Throughput Nerve Regeneration Studies In Caenorhabditis Elegans book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Parallelized Microfluidic Devices For High Throughput Nerve Regeneration Studies In Caenorhabditis Elegans
DOWNLOAD
Author : Navid Ghorashian
language : en
Publisher:
Release Date : 2010
Parallelized Microfluidic Devices For High Throughput Nerve Regeneration Studies In Caenorhabditis Elegans written by Navid Ghorashian and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
The nexus of engineering and molecular biology has given birth to high-throughput technologies that allow biologists and medical scientists to produce previously unattainable amounts of data to better understand the molecular basis of many biological phenomena. Here, we describe the development of an enabling biotechnology, commonly known as microfluidics in the fabrication of high-throughput systems to study nerve degeneration and regeneration in the well-defined model nematode, Caenorhabditis elegans (C. elegans). Our lab previously demonstrated how femtosecond (fs) laser pulses could precisely cut nerve axons in C. elegans, and we observed axonal regeneration in vivo in single worms that were immobilized on anesthetic treated agar pads. We then developed a microfluidic device capable of immobilizing one worm at a time with a deformable membrane to perform these experiments without agar pads or anesthetics. Here, we describe the development of improved microfluidic devices that can trap and immobilize up to 24 individual worms in parallel chambers for high-throughput axotomy and subsequent imaging of nerve regeneration in a single platform. We tested different micro-channel designs and geometries to optimize specific parameters: (1) the initial trapping of a single worm in each immobilization chamber, simultaneously, (2) immobilization of single worms for imaging and fs-laser axotomy, and (3) long term storage of worms on-chip for imaging of regeneration at different time points after the initial axon cut.
Parallel And Serial Microfluidic Platforms For Femtosecond Laser Axotomy In Caenorhabditis Elegans For Nerve Regeneration Studies
DOWNLOAD
Author : Sertan Kutal Gokce
language : en
Publisher:
Release Date : 2016
Parallel And Serial Microfluidic Platforms For Femtosecond Laser Axotomy In Caenorhabditis Elegans For Nerve Regeneration Studies written by Sertan Kutal Gokce and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Understanding the molecular basis of nerve regeneration can potentiate the development of novel and efficient treatments for neurodegenerative diseases. Severing axons in the small nematode Caenorhabditis elegans (C. elegans) with femtosecond laser surgery and then observing the subsequent axonal regrowth is a promising approach to understand the molecular mechanisms of nerve regeneration in vivo. Effective and reversible immobilization of the nematodes is necessary for both axotomy and follow-up imaging. However, conventional worm handling techniques such as using anesthetics or polystyrene beads are labor-intensive and time-consuming processes, hindering high-throughput. Microfluidic devices enable the manipulation of the nematodes on a single chip with unprecedented throughput and integrity. This dissertation introduces two comprehensive microfluidic systems for femtosecond laser axotomy in C. elegans, offering several advantages over conventional techniques in terms of speed and the ability for automation of the tedious axotomy experiments. The first microfluidic system is an automated serial microfluidic platform for performing femtosecond laser axotomy in C. elegans. The microfluidic platform along with a custom-developed automation program isolates a single nematode from a pre-loaded population, immobilizes the nematode, and performs femtosecond laser axotomy. The full automation of the axotomy process is achieved by combining efficient image analysis methodologies with synchronized valve and flow progression in the microfluidic chip to perform multiple surgeries in a serial and automated manner. The serial automated microfluidic platform reduces the time required to perform axotomies within individual worms to ~ 17 s/worm. The second microfluidic system is a parallelized multitrap microfluidic platform, “worm hospital”, that allows on-chip axotomy, post-surgery housing for recovery, and imaging of nerve regeneration on a single chip. The microfluidic platform features 20 trapping channels for laser axotomy and subsequent post-surgery imaging, and a perfusion area to house the worms after laser axotomy. This microfluidic platform is a single-flow Polydimethylsiloxane (PDMS) layer device and has no active control PDMS layer, which reduces the fabrication and operation complexity of the chip, especially for non-expert users. The roles of neurodevelopmental genes in the Wnt/Frizzled pathway on the nerve regeneration was investigated using the “worm hospital”. In summary, the microfluidic platforms presented in this dissertation enabled performing femtosecond laser axotomy in C. elegans in a fast and repeatable manner with a controllable microenvironment. Both microfluidic platforms offer promising methodologies for prospective large-scale screening of genes involved in nerve regeneration with a high throughput in an automated manner.
Automated Microfluidic Platforms To Facilitate Nerve Degeneration Studies With C Elegans
DOWNLOAD
Author : Navid Ghorashian
language : en
Publisher:
Release Date : 2013
Automated Microfluidic Platforms To Facilitate Nerve Degeneration Studies With C Elegans written by Navid Ghorashian and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
With its well-characterized genome, simple anatomy, and vast array of uses in molecular biology, the roundworm, Caenorhabditis elegans (C. elegans) is a well-established model organism in neurobiology. Concurrently, neurodegenerative diseases are some the most devastating and least understood ailments in modern medicine, making high-throughput approaches to understand their fundamental mechanisms imperative to developing new therapies. The worm's physical length-scales and simple genetics make it an ideal in vivo tool for high-throughput screening platforms. Concurrently, microfluidic technology has been used to make devices that manipulate these animals in a multitude of fashions to study various biological phenomena. With these considerations in mind, we have developed microfluidic platforms to facilitate optical interrogation of neurodegenerative and neuroregenerative phenomena in C. elegans for large-scale screens. First we developed a multiwell format device with 16 on-chip reservoirs to house and quickly deliver distinct worm populations to any liquid-format imaging platform. The system achieved unprecedented delivery speeds, avoided any population cross-contamination, and maintained animal viability. We then expanded this platform into a 64-well device that acted as a modular plug and play system for simple manipulation by conventional high-throughput liquid handling systems. The chip could be manipulated in the same fashion as a multiwell plate and interfaced with a novel pneumatic gasket system to achieve delivery speeds that were two-fold faster than those attained on the 16-well device. In addition, we worked to develop potential optical interrogation platform that could be fed populations of worms by the aforementioned delivery systems. This microfluidic chip consisted of an array of parallel traps to house individual worms over long durations for time-lapse studies of nerve regeneration after cuts to single axons mediated by a femtosecond pulse laser. Specifically, the platform was designed for regeneration studies in the C. elegans PQR neuron.
Automated Parallel Immobilization Microfluidic Platforms For High Throughput Neuronal Degeneration Studies With C Elegans
DOWNLOAD
Author : Evan Marley Hegarty
language : en
Publisher:
Release Date : 2014
Automated Parallel Immobilization Microfluidic Platforms For High Throughput Neuronal Degeneration Studies With C Elegans written by Evan Marley Hegarty and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
C. elegans has emerged as an invaluable model organism for in vivo neurobiology research to understand disease mechanisms and pathology relevant in humans. Simple anatomy, short lifecycle, fully characterized genome, and miniature body scale make these nematodes an ideal model organism for phenotyping and bio-molecular studies using microfluidic platforms. Advancements in soft-lithography have improved the functionality of microfluidic technology for C. elegans, leading to whole organism studies in high-throughput manner that were not otherwise possible. In order to study phenomena that require large amounts of data such as drug screens for neurological disorders and phenotyping, high-throughput imaging platforms with high-speed, high-resolution image acquisition become essential. With this in mind, we have developed and tested microfluidic immobilization devices to enable high-throughput optical interrogation of C. elegans for neurodegenerative diseases and large scale drug screens. Initially, we designed, developed, and tested single-layer and double-layer SU8 mold PDMS chips with parallel tapered channels to immobilize 40 adult C. elegans for high-resolution fluorescence imaging of their neurons in a parallel manner. vi We achieved over 90% immobilization efficiency using these initial devices, but could achieve only ~50% of the trapped worms with proper orientation to allow scoring of the VC neurons of interest. To improve worm orientation, we developed a three-layer microfluidic chip that can immobilize and orient the adult worms for optical interrogation of these VC neurons with 90% efficiency. Finally, we scaled the platform to accommodate a large scale platform with standard multi-well format on-chip wells where each well leads to the optimized trapping channels. The final optimized multi-well platform provides comprehensive easy to use 96-well microfluidic system to orient, immobilize, and image adult C. elegans in high-throughput manner. The novel gasket system can pressurize the multi-well device pre-loaded with 96 individual worm populations. Using a sequence of on-off applied gasket pressure, we can orient and immobilize worms in all 96 devices simultaneously in less than 5 minutes. Custom designed software can capture 12 z-stack images per worm from all 96-well in less than 12 minutes. With 95% trapping efficiency, approximately 90% of the worms can be scored successfully for neuronal phenotyping of VC neurons. This 96-well platform and the automated imaging system enable high-throughput optical interrogation of adult C. elegans for large-scale drug screens relating to ageing and various neurodegenerative diseases.
Micro And Nanosystems For Biotechnology
DOWNLOAD
Author : J. Christopher Love
language : en
Publisher: John Wiley & Sons
Release Date : 2016-03-07
Micro And Nanosystems For Biotechnology written by J. Christopher Love and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-03-07 with Science categories.
Emphasizing their emerging capabilities, this volume provides a strong foundation for an understanding of how micro- and nanotechnologies used in biomedical research have evolved from concepts to working platforms. Volume editor Christopher Love has assembled here a highly interdisciplinary group of authors with backgrounds ranging from chemical engineering right up to materials science to reflect how the intersection of ideas from biology with engineering disciplines has spurred on innovations. In fact, a number of the basic technologies described are reaching the market to advance the discovery and development of biopharmaceuticals. The first part of the book focuses on microsystems for single-cell analysis, examining tools and techniques used to isolate cells from a range of biological samples, while the second part is dedicated to tiny technologies for modulating biological systems at the scale of individual cells, tissues or whole organisms. New tools are described which have a great potential for (pre)clinical development of interventions in a range of illnesses, such as cancer and neurological diseases. Besides describing the promising applications, the authors also highlight the ongoing challenges and opportunities in the field.
Micro And Nano Systems For Biophysical Studies Of Cells And Small Organisms
DOWNLOAD
Author : Xinyu Liu
language : en
Publisher: Academic Press
Release Date : 2021-08-14
Micro And Nano Systems For Biophysical Studies Of Cells And Small Organisms written by Xinyu Liu and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-14 with Business & Economics categories.
Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms provides a comprehensive introduction to the state-of-the-art micro and nano systems that have recently been developed and applied to biophysical studies of cells and small organisms. These micro and nano systems span from microelectromechanical systems (MEMS) and microfluidic devices to robotic micro-nanomanipulation systems. These biophysical studies range from cell mechanics to the neural science of worms and Drosophila. This book will help readers understand the fundamentals surrounding the development of these tools and teach them the most recent advances in cellular and organismal biophysics enabled by these technologies. Comprehensive coverage of micro and nano-system technology and application to biophysical studies of cells and small organisms. Highlights the most recent advances in cellular and organismal biophysics enabled by micro and nano systems. Insightful outlook on future directions and trends in each chapter covering a sub-area of the book topic.
Molecular Diagnostics Promises And Possibilities
DOWNLOAD
Author : Mousumi Debnath
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-01-29
Molecular Diagnostics Promises And Possibilities written by Mousumi Debnath and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-01-29 with Medical categories.
A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.
Biomedical Membranes And Bio Artificial Organs
DOWNLOAD
Author : Stamatialis Dimitrios
language : en
Publisher: World Scientific
Release Date : 2017-11-29
Biomedical Membranes And Bio Artificial Organs written by Stamatialis Dimitrios and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11-29 with Technology & Engineering categories.
This book focusses on the development of biomedical membranes and their applications for (bio)artificial organs. It covers the state of art and main challenges for applying synthetic membranes in these organs. It also highlights the importance of accomplishing an integration of engineering with biology and medicine to understand and manage the scientific, industrial, clinical and ethical aspects of these organs. The compendium consists of 11 chapters, written by world renowned experts in the fields of membrane technology, biomaterials science and technology, cell biology, medicine and engineering. Every chapter describes the clinical needs and the materials, membranes, and concepts required for the successful development of the (bio)artificial organs. This text is suitable for undergraduate and graduate students in biomedical engineering, materials science and membrane science and technology, as well as, for professionals and researchers working in these fields. Contents: Controlled Drug Release Systems: Mechanisms and Kinetics (M Sanopoulou and K G Papadokostaki)Membranes for Artificial Kidneys (J Vienken)Advanced Blood Purification Therapies (O ter Beek, I Geremia, D Pavlenko and D Stamatialis)Membranes for Artificial Lung and Gas Exchange Applications (F Wiese)Membranes for Bioartificial Kidney Devices (N Chevtchik, P Caetano Pinto, R Masereeuw and D Stamatialis)Membrane-Based Bioartificial Liver Devices (S Khakpour, H M M Ahmed and L De Bartolo)Are Co-Culture Approaches Able to Improve Biological Functions of Bioartificial Livers? (V Pandolfi, U Pereira, M Dufresne and C Legallais)Membranes for Bioartificial Pancreas: Macroencapsulation Strategies (K Skrzypek, M G Nibbelink, M Karperien, A van Apeldoorn and D Stamatialis)Early Health Economic Evaluation of Bioartificial Organs: Involving Users in the Design of the Bioartificial Pancreas for Diabetes (M J IJzerman, T Wissing and E de Koning)Membranes for Regenerative Medicine in Clinical Applications (G F D'Urso Labate and G Catapano)Membranes for Organs-on-Chips (M P Tibbe, A D van der Meer, A van den Berg, D Stamatialis and L I Segerink) Readership: Researchers, professionals, undergraduate and graduate students in biomedical engineering, bioengineering and membrane science and technology. Keywords: Biomedical Membranes;Bioartificial;OrgansReview:0
Cell Based Biosensors
DOWNLOAD
Author : Qingjun Liu
language : en
Publisher: Artech House
Release Date : 2009-10
Cell Based Biosensors written by Qingjun Liu and has been published by Artech House this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10 with Medical categories.
Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations help clarify key topics throughout the book.
Computer Methods In Biomechanics And Biomedical Engineering
DOWNLOAD
Author : J. Middleton
language : en
Publisher: CRC Press
Release Date : 1996-03-18
Computer Methods In Biomechanics And Biomedical Engineering written by J. Middleton and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-03-18 with Medical categories.
These papers are concerned with new advances and novel solutions in the areas of biofluids, image-guided surgery, tissue engineering and cardovascular mechanics, implant analysis, soft tissue mechanics, bone remodeling and motion analysis. The contents also feature a special section on dental materials, dental adhesives and orthodontic mechanics. This edition contains many examples, tables and figures, and together with the many references, provides the reader with invaluable information on the latest theoretical developments and applications.