Pcr In Situ Hybridization
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Pcr 3
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Author : C. Simon Herrington
language : en
Publisher: Oxford University Press
Release Date : 1997-10-30
Pcr 3 written by C. Simon Herrington and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-10-30 with Science categories.
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
Pcr 3
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Author : C. S. Herrington
language : en
Publisher:
Release Date : 1997
Pcr 3 written by C. S. Herrington and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Pcr In Situ Hybridization
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Author : Gerard J. Nuovo
language : en
Publisher: Lippincott Williams & Wilkins
Release Date : 1997
Pcr In Situ Hybridization written by Gerard J. Nuovo and has been published by Lippincott Williams & Wilkins this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
Describes the technique whereby the extreme sensitivity of the polymerase chain reaction (PCR) is combined with the cell localizing ability of in situ hybridization. This revised and updated edition contains chapters on the basics of molecular biology; the nonspecific pathways of PCR; applications of PCR in situ hybridization--human papillomavirus, and HIV-1; and instrumentation. There is also an appendix on reagents for molecular biological analyses. Annotation copyright by Book News, Inc., Portland, OR
Pcr 3
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Author : C. S. Herrington
language : en
Publisher: Oxford University Press, USA
Release Date : 1998
Pcr 3 written by C. S. Herrington and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with DNA replication categories.
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chainreaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for researchand diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situhybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are furtherchapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-testedprotocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success,and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
Pcr In Situ Hybridization
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Author :
language : en
Publisher:
Release Date :
Pcr In Situ Hybridization written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Prins And In Situ Pcr Protocols
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Author : Franck Pellestor
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Prins And In Situ Pcr Protocols written by Franck Pellestor and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
In Situ Pcr And Related Technology
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Author : Jiang Gu
language : en
Publisher: Birkhäuser
Release Date : 2012-02-25
In Situ Pcr And Related Technology written by Jiang Gu and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-25 with Science categories.
Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology. The significance of this objective is obvious. A technique finally materialized in 1990 when Dr. Ashley T. Haase and coworkers published results that used multiple prim ers with complementary tails in intact cells. Since then, a number of laboratories have successfully developed their own versions of the technique. In situ peR is now a well-recognized method that permits the detection of minute quantities of DNA or RNA in intact cells or tissue sections. As a result, morphological analysis of those target nucleotide sequences becomes possible. As anticipated, this ad vancement has led to significant improvement in our understanding of many nor mal and abnormal conditions, and its impact is becoming more evident as time passes. In situ peR has the characteristics of a new landmark in morphologic technol ogy-it is scientifically fascinating and technically challenging. In essence, it is a combination of in situ hybridization and conventional peR. The wealth of litera ture, experience and protocols for the two latter techniques can be applied to in situ peR. In situ peR also has its own unique aspects that were not addressed by the other two techniques.
In Situ Hybridization
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Author : David G. Wilkinson
language : en
Publisher: OUP Oxford
Release Date : 1998-11-26
In Situ Hybridization written by David G. Wilkinson and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-26 with Science categories.
In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.
Pcr A Practical Approach 3 1998 Pcr In Situ Hybridization
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Author : C. S. Herrington
language : en
Publisher:
Release Date : 1998
Pcr A Practical Approach 3 1998 Pcr In Situ Hybridization written by C. S. Herrington and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with In situ hybridization categories.
In Situ Pcr Techniques
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Author : Omar Bagasra
language : en
Publisher: Wiley-Liss
Release Date : 1997-07-04
In Situ Pcr Techniques written by Omar Bagasra and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-07-04 with Science categories.
This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.