Pcr Primer

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Pcr Primer

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Author : Carl W. Dieffenbach
language : en
Publisher: CSHL Press
Release Date : 2003

Pcr Primer written by Carl W. Dieffenbach and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medical categories.

The Polymerase Chain Reaction (PCR) technique was invented nearly 20 years ago. Its subsequent variations and applications were many and varied, and today molecular biology, clinical, and forensic laboratories make almost daily use of PCR. This second edition of the much-praised PCR Primer: A Laboratory Manual updates the tried-and-true methods and presents the advances made in the 10 years since the first edition. After introducing the basics for PCR and methods of sample preparation, PCR Primer provides laboratory-tested protocols for RT-PCR methods, detection of PCR products, analysis of differential expression, cloning, and mutagenesis. These step-by-step methods include extensive background information, as well as valuable troubleshooting information provided by the leading experts in this technology. This manual is a comprehensive and reliable source of the full range of PCR methods for novices and experienced investigators alike.

Pcr Primer Design

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Author : Anton Yuryev
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Pcr Primer Design written by Anton Yuryev and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

Principles And Technical Aspects Of Pcr Amplification

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Author : Elizabeth van Pelt-Verkuil
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-14

Principles And Technical Aspects Of Pcr Amplification written by Elizabeth van Pelt-Verkuil and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-14 with Science categories.

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Pcr Primer Design

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Author : Chhandak Basu
language : en
Publisher: Humana
Release Date : 2016-10-08

Pcr Primer Design written by Chhandak Basu and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-08 with Medical categories.

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.

Bistro Primer Tool To Design And Validate Specific Pcr Primer Pairs For Phylogenetic Analysis

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Author : Praful Aggarwal
language : en
Publisher:
Release Date : 2004

Bistro Primer Tool To Design And Validate Specific Pcr Primer Pairs For Phylogenetic Analysis written by Praful Aggarwal and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with DNA polymerases categories.

Polymerase Chain Reaction is a widely used biological technique which helps in amplifying small quantities of DNA. These amplified DNA copies are then used in several other experiments like DNA sequencing, phylogenetic analysis, etc. PCR primers are short subsequences of nucleotides (basic unit of DNA) that help identify larger regions of the DNA sequence. They help in successfully amplifying the target DNA sequence by identifying complementary regions on the DNA template. Therefore, to successfully perform PCR it is imperative to design good quality primers. PCR can be used for identifying the phylogenetic classification of an organism. For example, in an anaerobic digester, there is a diverse microbial community involved that works to digest the waste material into carbon dioxide and methane. The methane produced can be used in the future as a renewable fuel. To identify the microbes involved, researchers use PCR that uses primer pair(s) targeting some specific group of microbes, on the 16S rRNA region of their sequences. This way they are more likely to amplify DNA from specific microbes only which are present in the target group. This could help in the phylogenetic classification of unknown microbes. In this thesis work a PCR primer pair design and validation software tool has been developed. This tool helps in designing primer pairs that amplify a target region in a specific taxonomic rank (e.g. Genus). It uses a novel scoring function to differentiate between the specific and the not-so specific primer pairs. 16S rRNA sequences for four different genera (Syntrophobacter, Syntrophomonas, Methanosarcina and Streptococcus) were used to develop and test the tool. To the best of my knowledge, primer pair(s) specific for amplifying Syntrophobacter or Syntrophomonas have not yet been published and the results from Bistro-Primer after further validation would be the first specific primer pairs for target amplification of these genera.

Pcr Cloning Protocols

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Author : Bing-Yuan Chen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

Pcr Cloning Protocols written by Bing-Yuan Chen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Pcr Protocols

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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02

Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications

Pcr

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Author : Mike McPherson
language : en
Publisher: Garland Science
Release Date : 2006-02-02

Pcr written by Mike McPherson and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-02 with Science categories.

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products a

Real Time Pcr

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Author : Kirstin J. Edwards
language : en
Publisher: Taylor & Francis
Release Date : 2004

Real Time Pcr written by Kirstin J. Edwards and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Polymerase chain reaction categories.

Pcr In Neuroscience

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Author :
language : en
Publisher: Elsevier
Release Date : 1995-04-13

Pcr In Neuroscience written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-04-13 with Science categories.

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) Complete retrieval of novel expressed genes by PCR without screening a library Quantitation by PCR Mutagenesis by PCR PCR in AIDS research Simple and effective protocols for PCR on archival specimens