Quantitative Real Time Pcr
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Quantitative Real Time Pcr
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Author : Roberto Biassoni
language : en
Publisher: Humana
Release Date : 2014-04-17
Quantitative Real Time Pcr written by Roberto Biassoni and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-17 with Science categories.
Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.
Real Time Pcr
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Author : M Tevfik Dorak
language : en
Publisher: Garland Science
Release Date : 2007-02-08
Real Time Pcr written by M Tevfik Dorak and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-08 with Science categories.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Rapid Cycle Real Time Pcr Methods And Applications
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Author : Carl Wittwer
language : en
Publisher: Springer
Release Date : 2012-12-06
Rapid Cycle Real Time Pcr Methods And Applications written by Carl Wittwer and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
Quantitative Real Time Pcr In Applied Microbiology
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Author : Martin Filion
language : en
Publisher: Caister Academic Press Limited
Release Date : 2012
Quantitative Real Time Pcr In Applied Microbiology written by Martin Filion and has been published by Caister Academic Press Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Microbiology categories.
Real time quantitative PCR (qPCR) technology has revolutionized almost all areas of microbiology, including clinical microbiology, food microbiology, industrial microbiology, environmental microbiology, and microbial biotechnology. Various modifications and improvements have enhanced the overall performance of this highly versatile technology and the qPCR instrumentation and strategies currently available are more sensitive, faster, and more affordable than ever before. Written by experts in the field and aimed specifically at microbiologists, this book describes and explains the most important aspects of current qPCR strategies, instrumentation, and software. Renowned scholars cover the application of qPCR technology in various areas of applied microbiology and comment on future trends. Topics include: instrumentation * fluorescent chemistries * quantification strategies * data analysis software * environmental microbiology * water microbiology * food microbiology * gene expression studies * validation of microbial microarray data * future trends in qPCR technology. This outstanding book will be invaluable for all microbiologists and is recommended for all microbiology laboratories.
Quantitative Real Time Pcr
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Author : Roberto Biassoni
language : en
Publisher:
Release Date : 2019
Quantitative Real Time Pcr written by Roberto Biassoni and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Polymerase chain reaction categories.
Gene Quantification
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Author : Francois Ferre
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Gene Quantification written by Francois Ferre and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Quantitative Real Time Rt Pcr Based Transcriptomics
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Author :
language : en
Publisher:
Release Date : 2006
Quantitative Real Time Rt Pcr Based Transcriptomics written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
Quantitative real-time polymerase chain reaction (qRT-PCR) is a new method for reliable quantification of low-abundance mRNA in biological samples. Since the strength of the fluorescence signal emitted by the report dye is proportional to the produced DNA amount, the fluorescence monitoring enables visualisation of the full reaction trajectory. The reaction trajectory can be then extrapolated back to an input concentration. RNA extraction can introduce unwanted contaminants into the sample, inhibiting the reverse transcription (RT) as well as the PCR reaction. These inhibitions cause then the reaction to precede sample-specific. In addition, the amplification efficiency varies not only between samples, but also along the recorded amplification trajectory of a single sample. Consequently, a correct determination of each probe’s PCR efficiency as well as a good standardization of the raw expression estimators is of great importance for a correct interpretation of results. To find a solution to above problems a series of biological experiments with RNA extracted from various ovine and bovine tissues and from cultured leukocytes was carried out. Constant amount of RNA was then reverse–transcribed to cDNA. All PCR runs were performed on a LightCycler instrument and Fluorescence data was saved in the LightCycler software. Based on this data, mathematical models together with statistical procedures were developed and validated. These can investigate the optimal quantification range and exactly determine its real-time PCR efficiency. Additionally, methods were developed to disclose heterogeneity between probes. All these procedures contribute to better quality of results obtained. Resulting from these standardisations, a decision algorithm for a proper analysis of the qRT-PCR data was designed.
Real Time Pcr
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Author : Kirstin J. Edwards
language : en
Publisher: Taylor & Francis
Release Date : 2004
Real Time Pcr written by Kirstin J. Edwards and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Polymerase chain reaction categories.
Real Time Pcr
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Author : Kirstin Edwards
language : en
Publisher: Taylor & Francis
Release Date : 2004
Real Time Pcr written by Kirstin Edwards and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
This essential manual presents a comprehensive guide to the most appropriate and up-to-date technologies and applications as well as providing an overview of the theory of this important technique. Written by recognized experts in the field this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: Real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection.
Rapid Cycle Real Time Pcr Methods And Applications
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Author : W. Dietmaier
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Rapid Cycle Real Time Pcr Methods And Applications written by W. Dietmaier and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Medical categories.
Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!