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Reconstitution Of Vesicular Transport In Saccharomyces Cerevisiae


Reconstitution Of Vesicular Transport In Saccharomyces Cerevisiae
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Reconstitution Of Vesicular Transport In Saccharomyces Cerevisiae


Reconstitution Of Vesicular Transport In Saccharomyces Cerevisiae
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Author : Thomas Wing Tung Yeung
language : en
Publisher:
Release Date : 1995

Reconstitution Of Vesicular Transport In Saccharomyces Cerevisiae written by Thomas Wing Tung Yeung and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.




Integration Of Transport Pathways In Saccharomyces Cerevisiae


Integration Of Transport Pathways In Saccharomyces Cerevisiae
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Author : Jesper Johansen
language : en
Publisher:
Release Date : 2017

Integration Of Transport Pathways In Saccharomyces Cerevisiae written by Jesper Johansen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.


Plasma membrane (PM) homeostasis is essential for viability and depends on maintaining a constant balance between the amount of membrane material arriving at the cell cortex and the amount recycled. This membrane transport is mediated by two distinct mechanisms: (i) vesicular transport, utilizing membrane-enclosed vesicles for bulk transport of membrane proteins and lipids; and (ii) non-vesicular lipid transport. While the molecular mechanisms of these processes are well defined, they are generally considered independent events but how they are integrated is poorly understood. To gain insight into how these transport pathways are coordinated at the PM, three avenues of research were conducted using a combination of genetic, biochemical and live-cell microscopy assays. First, I showed that the Oxysterol-binding protein-related protein (ORP) Osh4p, implicated in non-vesicular sterol transfer, was found to associate with exocytic vesicles and formed complexes with regulators of polarized exocytosis, including the small GTPases Sec4p, Cdc42p, and Rho1p. Second, I tested the function of the evolutionarily conserved endoplasmic reticulum (ER)-associated protein Arv1p, also suggested to be involved in non-vesicular sterol transfer. Ultimately Arv1p was found to be dispensable for sterol exchange between the ER and PM but instead it was shown to play an important role in maintaining ER ultrastructure; Arv1p might be involved in regulating insertion of tail-anchored proteins into membranes. Finally, the essential yeast Rab GTPase Sec4p, principally known to be a key regulator of exocytosis, was shown to mechanistically couple polarized exocytosis with cortical actin polymerization, which activates Las17p (the yeast Wiskott-Aldrich syndrome [WASp])-dependent endocytosis. Las17p activation results in actin filament nucleation, which pulls the PM inward for endocytic vesicle biogenesis. Sec4p thereby represents the first direct regulatory link that couples exocytosis and endocytosis, which we termed \"yeast compensatory endocytosis.\" By identifying novel mechanisms that coordinate intracellular transport pathways, these studies provided important new insights into how PM homeostasis is regulated and maintained.



Analyses Of Vesicular Transport Within The Endoplasmic Reticulum Golgi Interface In Saccharomyces Cerevisiae


Analyses Of Vesicular Transport Within The Endoplasmic Reticulum Golgi Interface In Saccharomyces Cerevisiae
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Author : Melanie Diefenbacher
language : en
Publisher:
Release Date : 2012

Analyses Of Vesicular Transport Within The Endoplasmic Reticulum Golgi Interface In Saccharomyces Cerevisiae written by Melanie Diefenbacher and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.




Vesicular Transport


Vesicular Transport
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Author :
language : en
Publisher: Academic Press
Release Date : 1989-10-04

Vesicular Transport written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-10-04 with Science categories.


Vesicular Transport, Part A



Molecular Biology And Genetic Engineering Of Yeasts


Molecular Biology And Genetic Engineering Of Yeasts
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Author : Henri Heslot
language : en
Publisher: CRC Press
Release Date : 1991-12-27

Molecular Biology And Genetic Engineering Of Yeasts written by Henri Heslot and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-12-27 with Technology & Engineering categories.


Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.



Seed Development And Germination


Seed Development And Germination
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Author : Jaime Kigel
language : en
Publisher: CRC Press
Release Date : 1995-01-04

Seed Development And Germination written by Jaime Kigel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-01-04 with Science categories.


This text is intended for plant physiologists, molecular biologists, biochemists, biotechnologists, geneticists, horticulturalists, agromnomists and botanists, and upper-level undergraduate and graduate students in these disciplines. It integrates advances in the diverse and rapidly-expanding field of seed science, from ecological and demographic aspects of seed production, dispersal and germination, to the molecular biology of seed development. The book offers a broad, multidisciplinary approach that covers both theoretical and applied knowledge.



Vesicular Transport In The Secretory And Endocytic Pathways


Vesicular Transport In The Secretory And Endocytic Pathways
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Author : Anne Müsch
language : en
Publisher: Biota Publishing
Release Date : 2014-09-01

Vesicular Transport In The Secretory And Endocytic Pathways written by Anne Müsch and has been published by Biota Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-01 with Science categories.


The concept of vesicular traffic as a means of protein transport in and out of cells and between membrane compartments has been established since the 1960s. Its basic principles are beautifully simple, yet the details of protein transport are complex enough to find novel classes of transport vesicles, and trafficking itineraries still being described to date. In this treatise, the reader will be introduced to mechanisms of vesicle sculpting, cargo selection, vesicle targeting, and vesicle consumption that have emerged as common characteristics of multiple transport steps in the exocytic and endocytic pathways. These fundamentals also reveal the basis for the specificity and selectivity of individual transport steps. We will further discuss how protein transport might lead to the establishment and maintenance of the endomembrane system of eukaryotic cells. The concepts for these mechanisms are based on experimental evidence combined with mathematical modeling that can disclose the minimal requirements for the generation of the distinct membrane compartments. Table of Contents: Compartmentalization Is a Hallmark of Eukaryotic Cells / Organelles Can Be Classified Based on Their Origin / There Are Multiple Entry Points Into the Endomembrane System and a Network of Connecting Trafficking Routes / There Are Exceptions and Extensions to the Classic Trafficking Itineraries / A Mathematical Model Can Explain How Organelle Size Is Established and Maintained / Coats and SNAREs Constitute the Core Machinery for Vesicle Budding and Fusion / A Mathematical Model Explains How Coats and SNAREs Are Sufficient to Generate Organelle Identity / How Vesicle Formation Is Linked to Cargo Incorporation / RabGTPases Are the Master Regulators of Vesicular Traffic / The Mechanisms and Physiological Roles of Endocytosis / Models for Protein Transport in the Golgi Apparatus / Summary / Acknowledgments / References / Author Biography



Protein Targeting Transport And Translocation


Protein Targeting Transport And Translocation
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Author : Ross Dalbey
language : en
Publisher: Elsevier
Release Date : 2002-04-09

Protein Targeting Transport And Translocation written by Ross Dalbey and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-04-09 with Science categories.


Protein Targeting, Transport, and Translocation presents an in-depth overview on the topic of protein synthesis, covering all areas of protein science, including protein targeting, secretion, folding, assembly, structure, localization, quality control, degradation, and antigen presentation. Chapters also include sections on the history of the field as well as summary panels for quick reference. Numerous color illustrations complement the presentation of material. This book is an essential reference for anyone in biochemistry and protein science, as well as an excellent textbook for advanced students in these and related fields. Basic principles and techniques Targeting adn sorting sequences Protein export in bacteria Membrane protein integration into ER and bacterial membranes Protein translocation across the ER Disulfide bond formation in prokaryotes and eukaryotes Quality control in the export pathway Import of proteins into organelles The secretory pathway Vesicular transport Spectacular color throughout



Advances In Microbial Physiology


Advances In Microbial Physiology
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Author :
language : en
Publisher: Academic Press
Release Date : 1977-08-24

Advances In Microbial Physiology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977-08-24 with Science categories.


Advances in Microbial Physiology publishes topical and important reviews, interpreting physiology to include all material that contributes to our understanding of how microorganisms and their component parts work.



Gtpases


Gtpases
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Author : Alan Hall
language : en
Publisher: Oxford University Press, USA
Release Date : 2000

Gtpases written by Alan Hall and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Language Arts & Disciplines categories.


GTPases are molecular switches that are used to control biochemical pathways. This book describes the properties and cellular roles of all the major families of GTPases. All cells use GTPases to regulate the delivery of amino acids to the ribosome during protein synthesis, but eukaryotes, with their complex and compartmentalized environment, have exploited the versatility of GTPases to a much greater extent. In particular, GTPases play a central role in regulating signal transduction pathways activated by extracellular signals and in regulating the trafficking of proteins beetween different organelles. It is not surprising, therefore, that GTPases have become a center of attention for those studying the control of proliferation, differentiation, cell polarity, cell movement, and vesicle and protein trafficking. This book provides a complete guide to this area and should be essential reading for cell and molecular biologists, biochemists and geneticists interested in these contemporary problems.