Rna Protein Interactions
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Rna Protein Interactions A Practical Approach
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Author : Christopher W.J. Smith
language : en
Publisher: Oxford University Press, UK
Release Date : 1998-07-09
Rna Protein Interactions A Practical Approach written by Christopher W.J. Smith and has been published by Oxford University Press, UK this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-07-09 with categories.
RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -
Rna Protein Interaction Protocols
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Author : Susan R. Haynes
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Rna Protein Interaction Protocols written by Susan R. Haynes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The molecular characterization of RNA and its interactions with proteins is an important and exciting area of current research. Organisms utilize a variety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscriptional processes are critical components of gene regulation has sparked an explosion of interest in both stable ribonucleoprotein (RNP) complexes and transient RNA–protein interactions. RNA is conformationally flexible and can adopt complex structures that provide diverse surfaces for interactions with proteins. The fact that short RNA molecules (aptamers; see Chapter 16) can be selected to bind many different types of molecules is evidence of the structural variability of RNA. RNA molecules are rarely entirely single- or double-stranded, but usually contain multiple short duplexes interrupted by single-stranded loops and bulges; in some RNAs, such as tRNAs, the short duplexes stack on each other. Further variability is generated by the presence of non-Watson-Crick base pairs, modified nucleotides, and more complex structures, such as pseudoknots and triple-strand interactions.
Rna Protein Complexes And Interactions
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Author : Ren-Jang Lin
language : en
Publisher: Springer Nature
Release Date : 2023-05-11
Rna Protein Complexes And Interactions written by Ren-Jang Lin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-05-11 with Science categories.
This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.
Biophysics Of Rna Protein Interactions
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Author : Chirlmin Joo
language : en
Publisher: Springer Nature
Release Date : 2019-09-19
Biophysics Of Rna Protein Interactions written by Chirlmin Joo and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-19 with Science categories.
RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.
Rna Protein Interactions
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Author : Kiyoshi Nagai
language : en
Publisher: IRL Press
Release Date : 1994
Rna Protein Interactions written by Kiyoshi Nagai and has been published by IRL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Science categories.
Many biological processes rely absolutely on interactions between the two most abundant large-molecule living organisms, proteins and RNA. The basis of these interactions is, however, very poorly understood. This study provides a detailed and up-to-date overview.
Rna Protein Interactions
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Author : BMB 307
language : en
Publisher:
Release Date :
Rna Protein Interactions written by BMB 307 and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Quantitative Modeling Of Rna Protein Interactions
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Author : Salma Sohrabi-Jahromi
language : en
Publisher:
Release Date : 2021
Quantitative Modeling Of Rna Protein Interactions written by Salma Sohrabi-Jahromi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.
RNA-binding proteins (RBPs) impact every aspect of RNA metabolism including RNA transcription, maturation, export, localization, translation, and stability. Specific RNA-protein interactions therefore play a central role in regulating many cellular processes. However, most RBPs preferentially bind short, often degenerate sequence motifs (∼3-5 bases) that alone cannot explain how they target only specific subsets of transcripts in the cell. In this thesis, I report on the analysis and the thermodynamic modeling of RNA-protein interaction datasets, with the aim of cracking the code behind RBP...
Rna Protein Interactions
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Author : Kiyoshi Nagai
language : en
Publisher: Oxford University Press, USA
Release Date : 1994
Rna Protein Interactions written by Kiyoshi Nagai and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
Protein Nucleic Acid Interactions
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Author : Phoebe A. Rice
language : en
Publisher: Royal Society of Chemistry
Release Date : 2008-05-22
Protein Nucleic Acid Interactions written by Phoebe A. Rice and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-05-22 with Science categories.
This book provides both in-depth background and up-to-date information in this area. The chapters are organized by general themes and principles, written by experts who illustrate topics with current findings. Topics covered include: - the role of ions and hydration in protein-nucleic acid interactions - transcription factors and combinatorial specificity - indirect readout of DNA sequence - single-stranded nucleic acid binding proteins - nucleic acid junctions and proteins, - RNA protein recognition - recognition of DNA damage. It will be a key reference for both advanced students and established scientists wishing to broaden their horizons.
Symposium On Rna Biology
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Author :
language : en
Publisher:
Release Date : 1995
Symposium On Rna Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.