Schmidtea Mediterranea
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Schmidtea Mediterranea
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Author : Luca Gentile
language : en
Publisher: Springer Nature
Release Date : 2023-07-10
Schmidtea Mediterranea written by Luca Gentile and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-07-10 with Science categories.
This detailed volume examines fine-tuned methodologies using the planarian species, Schmidtea mediterranea. The book features experimental protocols covering topics from in situ hybridization, immunohistochemistry, cell dissociation and flow cytometry, to pipelines for the analysis of large datasets, as in genomics and transcriptomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schmidtea mediterranea: Methods and Protocols provides both experts in the field and newcomers with the best possible toolbox for their everyday lab work utilizing this valuable model.
Investigating Head Regeneration In The Planarian Schmidtea Mediterranea
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Author :
language : en
Publisher:
Release Date : 2014
Investigating Head Regeneration In The Planarian Schmidtea Mediterranea written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Anterior Regeneration Of Schmidtea Mediterranea
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Author : Deborah Jane Evans
language : en
Publisher:
Release Date : 2011
Anterior Regeneration Of Schmidtea Mediterranea written by Deborah Jane Evans and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Planarians can regenerate; significantly this includes the regeneration of a head with a functional brain upon decapitation. In this thesis classical cutting experiments were revisited in the planarian species S. mediterranea. A schedule of events that follow the same temporal dynamics between anterior blastemas originating from amputations from different AlP locations is uncovered. This schedule is delayed at the early stages of regeneration at more posterior levels. To investigate the molecular mechanisms underlying this delay, the roles of Hh and canonical Wnt signalling, previously implicated in planarian anteroposterior (A/P) specification, were explored. Anterior regeneration rates are dependent on canonical Wnt signalling, whilst the gradation of anterior regeneration rates over the A/P axis is dependent on Hh signalling In contrast, brain primordia differentiation occurs independently of Hedgehog (Hh) and canonical Wnt signalling and also exhibits a temporal anterior to posterior graded appearance. A combination of cell cycle inhibitor treatments, double amputation strategies and ribonucleic acid (RNA) mediated interference (RNAi) experiments indicate that these brain primordia are formed by dividing neoblasts. These cells are past S-phase of the cell cycle and are specified to form brain primordial fate in the vicinity of the anterior facing wound within the first 16 hours of regeneration, even if this region will eventually form tail structures. The data presented here is consistent with these cells being either at G2 or M of the cell cycle at the time of amputation, or even possibly post-mitotic neoblast progeny that are still able to respond to an anterior wound to become brain fated cells. In addition the blastema/post-blastema positions that brain primordia differentiate at are dependent on existing canonical Wnt signalling. In conclusion, ectopic Hh signalling results in a transition from anterior to posterior polarity, whilst ectopic canonical Wnt signalling leads to posterior polarity from the onset of anterior regeneration. Later, two-tailed Smed-ptc(RNAi) and Smed-APC-1(RNAi) animals regenerate previously unreported brain structures. Smed-ptc(RNAi) animals develop centrally positioned outgrowths with cephalic identity whilst Smed-APC-1 (RNAi) animals develop peri-pharyngeal brain primordia-like structures. In Smed-ptc(RNAi) animals ectopic Hh signalling at anterior facing wounds specify posterior organisers which instigate a ~catenin-1 activity gradient through which an outgrowth with cephalic identity is regenerated. In contrast Smed-APC- 1(RNAi) animals have globally high levels of ~catenin-1 activity which prevent an outgrowth forming. Canonical Wnt signalling is shown to instruct AlP polarity of new and existing tissue, intact Smed-hh(RNAi) and Smed-ptc(RNAi) animals do not display AlP disruption. This implicates a regeneration specific role of Hh signalling during AlP polarity establishment. These results elaborate on the current models of both AlP axis and brain regeneration and have broad implications for understanding regenerative processes and the molecular networks that control them. Here two modes of brain tissue regeneration are proposed; early brain primordia are controlled by mechanisms that are independent of canonical Wnt signalling whilst later low ~catenin-1 activity levels are required for the regeneration of elaborated brains.
Neoblast And Germ Cell Dynamics In The Planarian Schmidtea Mediterranea
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Author : Mette Handberg-Thorsager
language : en
Publisher:
Release Date : 2008
Neoblast And Germ Cell Dynamics In The Planarian Schmidtea Mediterranea written by Mette Handberg-Thorsager and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Investigating Head Regeneration In The Planarian Schmidtea Mediterranea
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Author : Matthias Christian Vogg
language : en
Publisher:
Release Date : 2014
Investigating Head Regeneration In The Planarian Schmidtea Mediterranea written by Matthias Christian Vogg and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Developing Tools For The Study Of Embryogenesis In The Planarian Schmidtea Mediterranea
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Author : Heather Skye Comstra
language : en
Publisher:
Release Date : 2010
Developing Tools For The Study Of Embryogenesis In The Planarian Schmidtea Mediterranea written by Heather Skye Comstra and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
Proteomic Profiling Of The Planarian Schmidtea Mediterranea And Its Mucous Reveals Similarities With Human Secretions And Those Predicted For Parasitic Flatworms
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Author : Donald Gerald Bocchinfuso
language : en
Publisher:
Release Date : 2012
Proteomic Profiling Of The Planarian Schmidtea Mediterranea And Its Mucous Reveals Similarities With Human Secretions And Those Predicted For Parasitic Flatworms written by Donald Gerald Bocchinfuso and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Pbc And Meis Class Genes In The Planarian Schmidtea Mediterranea
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Author : Daniel André Felix
language : en
Publisher:
Release Date : 2011
Pbc And Meis Class Genes In The Planarian Schmidtea Mediterranea written by Daniel André Felix and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Planarians have outstanding regenerative capabilities which depend on the presence of a pool of somatic stem cells. In addition planarians keep perfect proportion of the body while degrowing during starvation. While the establishment and maintenance of the anteroposterior (AlP) axis has been shown to depend on Wnt and Hedgehog signalling, not much is known about the subsequent patterning events. PBC and MEIS class genes are evolutionary conserved TALE homeobox genes with important functions in patteming and cell differentiation. They are well known co-factors of the Hox genes however they also have other Hox-independent functions. The aim of this study was to identify the planarian orthologs of the PBC and MEIS class genes and to examine their putative role in anteroposterior patteming. Here one PBC class gene, Smed-pbx, and the three MEIS class genes Smed-meis I , Smed-meis2 and Smed-prep were identified and orthology established through phylogenetic analyses. Smed-prep was found to define the cephalic area in which the anterior structures are formed, most prominently the brain. In anterior regenerating Smed-prep(RNAi) worms a reduction or even complete absence of the brain and anterior markers was observed. Importantly, the differentiation of stem cells into nerve cells was shown not to be dependent on Smed-prep. In step with its function the Smed-prep gene was found to have an anteroposterior bias in expression, being highly expressed in the head. While both Smed-meis genes were found to be important for regeneration and maintenance of the eyes and for the adaptation of the body to the new size of the animal after amputation, Smed-pbx was found to have pleiotropic functions, phenocopying aspects of all three MEIS class genes upon RNAi interference, and also producing additional phenotypes. This study has found that the four planarian orthologs of PBC and MEIS class genes are important players in regeneration. Two genes, Smed-pbx and especially Smed-prep, have been found to be necessary for anteroposterior patterning. This is the first time that a homeobox transcription factor has been directly implicated in anteroposterior patterning in planarians.
Somatic Regulation Of Germ Cell Development In The Planarian Schmidtea Mediterranea The Role Of Systemic And Local Factors
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Author : Amir Saberi
language : en
Publisher:
Release Date : 2016
Somatic Regulation Of Germ Cell Development In The Planarian Schmidtea Mediterranea The Role Of Systemic And Local Factors written by Amir Saberi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Transcriptome Assembly And Analysis Of The Freshwater Planarian Schmidtea Mediterranea
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Author : Damian Kao
language : en
Publisher:
Release Date : 2014
Transcriptome Assembly And Analysis Of The Freshwater Planarian Schmidtea Mediterranea written by Damian Kao and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Gene expression categories.