Selectivity And Structure Of Chimeric Loop Swaps In Sh2 Domains
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Selectivity And Structure Of Chimeric Loop Swaps In Sh2 Domains
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Author : Sarah N. Smith
language : en
Publisher:
Release Date : 2023
Selectivity And Structure Of Chimeric Loop Swaps In Sh2 Domains written by Sarah N. Smith and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with Chemical structure categories.
SH2 (Src Homology 2) domains are protein domains that bind to phosphorylated tyrosine residues within cell signaling cascades. They have been found to play a role in certain cancers and immunological disorders. Despite their importance in cell signaling and medical relevance, the structural basis of the various selectivity classes of SH2 domains is only partially understood. Previous research found that the EF and BG loops of the domains contribute to forming the peptide binding pocket, and thus impact their selectivity. To further understand the role of these loops in selectivity, we engineered chimeric SH2 domains by swapping the EF and BG loops from other SH2 domains into the backbone of c-Src SH2. Methods of fluorescence polarization, Ki binding assays, computer modeling, and X-ray crystallography were used to test if the loops can alter selectivity and structure. We found that the chimeras had a lower affinity for the *pYEEI (pY refers to phosphorylated tyrosine) peptide than the wild-type Src-SH2 domain, showing the loop swap alters the disassociation constant of the chimeras for this target peptide. Broad variability was also observed through Ki competition assays. The structures of several chimeras were also visualized through computational molecular modeling, suggesting alterations in the structure of the binding pocket. These results provide further evidence for these loops contributing to the selectivity of SH2 domains, and a better understanding of how these domains function.
Inhibitors Of Protein Kinases And Protein Phosphates
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Author : Denis Alexander
language : en
Publisher: Handbook of Experimental Pharmacology
Release Date : 2004-10-04
Inhibitors Of Protein Kinases And Protein Phosphates written by Denis Alexander and has been published by Handbook of Experimental Pharmacology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-10-04 with Medical categories.
with contributions by numerous experts
Chemical Abstracts
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Author :
language : en
Publisher:
Release Date : 2002
Chemical Abstracts written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Chemistry categories.
Cumulated Index Medicus
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Author :
language : en
Publisher:
Release Date : 1995
Cumulated Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Medicine categories.
Introduction To Protein Structure
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Author : Carl Ivar Branden
language : en
Publisher: Garland Science
Release Date : 2012-03-26
Introduction To Protein Structure written by Carl Ivar Branden and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-26 with Science categories.
The VitalBook e-book of Introduction to Protein Structure, Second Edition is inly available in the US and Canada at the present time. To purchase or rent please visit http://store.vitalsource.com/show/9780815323051Introduction to Protein Structure provides an account of the principles of protein structure, with examples of key proteins in their bio
From Protein Structure To Function With Bioinformatics
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Author : Daniel John Rigden
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-12-11
From Protein Structure To Function With Bioinformatics written by Daniel John Rigden and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-11 with Science categories.
Proteins lie at the heart of almost all biological processes and have an incredibly wide range of activities. Central to the function of all proteins is their ability to adopt, stably or sometimes transiently, structures that allow for interaction with other molecules. An understanding of the structure of a protein can therefore lead us to a much improved picture of its molecular function. This realisation has been a prime motivation of recent Structural Genomics projects, involving large-scale experimental determination of protein structures, often those of proteins about which little is known of function. These initiatives have, in turn, stimulated the massive development of novel methods for prediction of protein function from structure. Since model structures may also take advantage of new function prediction algorithms, the first part of the book deals with the various ways in which protein structures may be predicted or inferred, including specific treatment of membrane and intrinsically disordered proteins. A detailed consideration of current structure-based function prediction methodologies forms the second part of this book, which concludes with two chapters, focusing specifically on case studies, designed to illustrate the real-world application of these methods. With bang up-to-date texts from world experts, and abundant links to publicly available resources, this book will be invaluable to anyone who studies proteins and the endlessly fascinating relationship between their structure and function.
Drosophila Melanogaster Practical Uses In Cell And Molecular Biology
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Author :
language : en
Publisher: Academic Press
Release Date : 1995-01-25
Drosophila Melanogaster Practical Uses In Cell And Molecular Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-01-25 with Science categories.
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future. Key Features* Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster* Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques
Protein Kinase Functions
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Author : James Robert Woodgett
language : en
Publisher: Oxford University Press, USA
Release Date : 2000
Protein Kinase Functions written by James Robert Woodgett and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.
Since the publication of Protein Kinases in 1994 many novel protein kinases have been discovered, but perhaps more importantly there have been dramatic advances in our understanding of the cellular functions of this remarkably diverse class of proteins. Protein Kinase Functions is not just an update of the previous edition but provides a new focus on the context and function of protein kinases, thus reflecting the recent advances in kinase biology. Chapters on genetic approaches to protein kinase functions, the MAP kinase pathway, and cyclin-dependent kinases have been completely updated and new topics covered in depth are: phosphatidylinositol 3-kinase signalling, JAK-STAT signalling, suppression of tyrosine kinases by the SOCS family proteins, the TGF� superfamily, and the involvement of protein kinases in response to DNA damage. Throughout, emphasis is placed not on individual kinases, but on the functional aspects of the whole system and the relationship between processes and molecules. It is the aim of Protein Kinase Functions to enable the reader to assimilate, compare, and integrate the molecular machinery used by cells to co-ordinate and respond to their environments.
Intercellular Communication Through Gap Junctions
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Author : Y. Kanno
language : en
Publisher: Newnes
Release Date : 1995-02-09
Intercellular Communication Through Gap Junctions written by Y. Kanno and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-02-09 with Science categories.
Research on intercellular communication through gap junctions has continued to expand, and the meeting on which this book is based brought together many scientists from many different countries and disciplines. In line with the objective of the meeting, this volume focuses on the biological meaning of intercellular communication through gap junctions in various organs. The most recent up-to-date findings have been included in this extensive volume, valuable to all those interested in this rapidly expanding field.
Universities Press Dictionary Of Biotechnology And Genetic Engineering
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Author : Sharon D. Cosloy
language : en
Publisher: Universities Press
Release Date : 2003
Universities Press Dictionary Of Biotechnology And Genetic Engineering written by Sharon D. Cosloy and has been published by Universities Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Biochemical engineering categories.
Revised And Expanded By More Than 300 New Terms, The Universities Press Dictionary Of Biotechnology And Genetic Engineering, New Edition Is An Essential Reference Tool On Modern Biotechnology And Genetic Engineering That Lucidly Articulates The Flood Of Advances And Discoveries In These Areas. In Addition To 100 Black-And-White Line Drawings, The Dictionary Includes Four Helpful Appendixes. The Universities Press Dictionary Of Biotechnology And Genetic Engineering, New Edition Will Benefit Students, Teachers, Physicians, Science And Technical Writers, Or Others Looking For A Concise Source Of Current Information On These Interdisciplinary Fields.