Solid State Nanopores
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Solid State Nanopores
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Author : Kyle Briggs
language : en
Publisher:
Release Date : 2018
Solid State Nanopores written by Kyle Briggs and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
The work presented in this thesis is divided loosely into three main areas of interest: development of a novel method of solid-state nanopore fabrication; applications of this method to some of the open problems in the field; and analysis of nanopore data. The first of these occupies the majority of the research presented in this thesis, covering research dedicated to the development and characterization of a novel method of solid-state nanopore fabrication which achieves nanometer scale control over matter using simple and low cost circuitry. Termed controlled breakdown (CBD), this technique is in the process of revolutionizing the field of nanopore research, and in the few short years I have been part of its development it has seen adoption in nanopore labs across the globe, both academic and industrial. Due to the simple nature of CBD, this technique also enables novel applications of nanopores in device architectures that were inaccessible to the expensive and inflexible methods used previously. The second part of this thesis takes advantage of the unique opportunities presented by CBD to develop a device architecture comprising two nanopores in series. This nanodevice tackles one of the main problems standing between nanopores and the promise of cheap genomic analysis: control of the motion and conformation of the polymer both prior to and during translocation through the pore. Finally, because the field of nanopore research is still relatively young, very few tools are available which provide high-quality analysis of nanopore data. The last part of this thesis is dedicated to a thorough discussion of the complexities involved in analysing nanopore signals, as well as the development of several tools which directly address this knowledge gap.
Solid State Nanopores
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Author : Jean-Pierre Leburton
language : en
Publisher: Springer Nature
Release Date : 2023-09-30
Solid State Nanopores written by Jean-Pierre Leburton and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-30 with Technology & Engineering categories.
This contributed volume provides an overview of the recent advances in solid-state nanopore technology, featuring contributions by leading experts in the field. It discusses several aspects of solid-state nanopores, covering their fabrication as well as multiple biosensing applications. It successfully bridges the gap between various scientific and engineering disciplines and highlights the progress made in this area. This title is a useful tool for acquiring basic knowledge of this field and following recent progress. It is a valuable contribution to the area of nanopore biosensing and is of interest to graduate students, postdocs, or senior researchers working in the fields of physical chemistry, biochemistry, bio- and electrical engineering, and biophysics.
Nanopores
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Author : Samir M. Iqbal
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-07-12
Nanopores written by Samir M. Iqbal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-12 with Technology & Engineering categories.
Nanopores are nanometer scale holes formed naturally by proteins or cells, and can be used for a variety of applications, including sequencing DNA and detecting anthrax. They can be integrated into artificially constructed encapsulated cells of silicon wafers while allowing small molecules like oxygen, glucose and insulin to pass, while keeping out large system molecules. "Nanopores: Sensing and Fundamental Biological Interactions" examines the emerging research directions surrounding nanopores such as genome sequencing and early disease detection using biomarker identification. Covering the applications of nanopores in genetics, proteomics, drug discovery, early disease detection and detection of emerging environmental threats, it is a must-have book for biomedicalengineers and research scientists.
Fabrication And Characterization Of Thinner Solid State Nanopores
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Author : Denis Forbi Tita
language : en
Publisher:
Release Date : 2012
Fabrication And Characterization Of Thinner Solid State Nanopores written by Denis Forbi Tita and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Biomedical engineering categories.
Solid State nanopores that are fabricated by the ion beam sculpting process and electron beam drilling have shown great promise as a sensing device for DNA and protein molecules. Even though biological pores such as the alpha-Haemolysin have been in use for quite some time, the use of solid state Nanopores in single biomolecule detection has been on the rise since the mid 1990s. Solid State nanopores have an advantage over biological pores in that they are more robust, stable, and can be sculpted to any desired size for use in translocation experiments. One of the major challenges in Nanopore fabrication by ion beam sculpting has been limited by the user's ability to control the closure rate of pores in the fabrication process. Another challenge in nanopore sensing is the resolution limitation due to the thickness of the pore. This is because most of the nanopores fabricated by the ion beam sculpting method are often thicker than they should. This thesis will focus on the modification of nanopore fabrication using the ion beam sculpting system at the University of Arkansas by first baking the samples in vacuum under specified temperature conditions. Baking the samples will give the user better control over pore closure. This Thesis will also focus on thinning the sculpted pores by Reactive Ion Etching in an attempt to increase its resolution for single biomecule translocation experiments.
New Approach In Fabrication Of Solid State Nanopore For Bio Sensing Applications
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Author : Wing Hei Harold Kwok
language : en
Publisher:
Release Date : 2015
New Approach In Fabrication Of Solid State Nanopore For Bio Sensing Applications written by Wing Hei Harold Kwok and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Engineered Nanopores For Bioanalytical Applications
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Author : Joshua B. Edel
language : en
Publisher: William Andrew
Release Date : 2013-03-19
Engineered Nanopores For Bioanalytical Applications written by Joshua B. Edel and has been published by William Andrew this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-19 with Science categories.
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding. This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials Introduces the techniques of manufacturing solid state materials with functional nanopores Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Dna Characterization With Solid State Nanopores And Combined Carbon Nanotube Across Solid State Nanopore Sensors
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Author : Dimitar Mihaylov Vlassarev
language : en
Publisher:
Release Date : 2012
Dna Characterization With Solid State Nanopores And Combined Carbon Nanotube Across Solid State Nanopore Sensors written by Dimitar Mihaylov Vlassarev and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
A DNA molecule passing through a nanopore in a liner and sequential fashion allows for unprecedented interrogation of the polymer. Adding transverse electrodes that are comparable in size and sensitive to the DNA molecule, can further the attempts to rapidly sequence DNA.
Trapping Single Molecules With A Solid State Nanopore
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Author : Marc Herman Gershow
language : en
Publisher:
Release Date : 2008
Trapping Single Molecules With A Solid State Nanopore written by Marc Herman Gershow and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Molecular probes categories.
I describe a system for measuring and manipulating single molecules using a solid state nanopore, a small hole in an insulating film fashioned using solid-state Silicon processing techniques. In this work, pores less than 10 nanometers in diameter in 20-30 nm thick Silicon Nitride membranes are used to join two reservoirs of salt water. DNA molecules added to one reservoir are forced through the nanopore by an applied voltage bias. Single molecules of DNA are detected passing through the pore by a blockage of the ionic current between the reservoirs. The depth and duration of the current blockages reveal information about the molecule's geometry while the shape of the blockage depends on the molecule's conformation. If the driving voltage is reversed soon after a molecule has passed through the nanopore, the same molecule can be "recaptured," induced to pass through the pore a second time. This process reveals information about the dynamics of the molecule outside of and its interaction with the nanopore. The recapture process can be repeated multiple times to form a single molecule trap.
Precise Size Control And Noise Reduction Of Solid State Nanopores For The Detection Of Dna Protein Complexes
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Author : Eric Beamish
language : en
Publisher:
Release Date : 2012
Precise Size Control And Noise Reduction Of Solid State Nanopores For The Detection Of Dna Protein Complexes written by Eric Beamish and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Biochemical markers categories.
Over the past decade, solid-state nanopores have emerged as a versatile tool for the detection and characterization of single molecules, showing great promise in the field of personalized medicine as diagnostic and genotyping platforms. While solid-state nanopores offer increased durability and functionality over a wider range of experimental conditions compared to their biological counterparts, reliable fabrication of low-noise solid-state nanopores remains a challenge. In this thesis, a methodology for treating nanopores using high electric fields in an automated fashion by applying short (0.1-2 s) pulses of 6-10 V is presented which drastically improves the yield of nanopores that can be used for molecular recognition studies. In particular, this technique allows for sub-nanometer control over nanopore size under experimental conditions, facilitates complete wetting of nanopores, reduces noise by up to three orders of magnitude and rejuvenates used pores for further experimentation. This improvement in fabrication yield (over 90%) ultimately makes nanopore-based sensing more efficient, cost-effective and accessible. Tuning size using high electric fields facilitates nanopore fabrication and improves functionality for single-molecule experiments. Here, the use of nanopores for the detection of DNA-protein complexes is examined. As proof-of-concept, neutravidin bound to double-stranded DNA is used as a model complex. The creation of the DNA-neutravidin complex using polymerase chain reaction with biotinylated primers and subsequent purification and multiplex creation is discussed. Finally, an outlook for extending this scheme for the identification of proteins in a sample based on translocation signatures is presented which could be implemented in a portable lab-on-a-chip device for the rapid detection of disease biomarkers.
Membrane Transfer Process For The Creation Of Low Noise Solid State Nanopore Devices
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Author : Tarun Kumar Jain
language : en
Publisher:
Release Date : 2011
Membrane Transfer Process For The Creation Of Low Noise Solid State Nanopore Devices written by Tarun Kumar Jain and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Nanopore sensors are an emerging technology whereby the modulation of a current trace upon passage of the analyte through the nanopore is used to infer the properties of the analyte. This technology is particularly attractive because it is label-free, rapid, and intrinsically single molecule. However, the ability to infer molecular properties with nanometer precision, either in size or sequencing, is obscured by noise in the measurement. The precision of this inference can be significantly improved by reducing noise and by performing multiple measurements on the same molecule. Solid state nanopores, made in free standing membranes, however, have traditionally exhibited high levels of capacitive noise at 100 kHz bandwidths, as well as poor confinement of the electric field around the nanopore. In this thesis, a novel device concept is designed for creating solid state nanopores whereby the free standing membrane is transferred over a PDMS microchannel. By eliminating the silicon wafer backing, capacitive noise is dramatically reduced. Furthermore, the microchannel confines the electric field outside the nanopore, thereby enhancing the ability to perform multiple measurements.
