The Influence Of Hypoxia On Gata 1 And Epo Expression Levels In Developing Zebrafish
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The Influence Of Hyproxia An Gata 1 And Epo Expression Levels In Developing Zebrafish
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Author : Markus Holotta
language : en
Publisher: diplom.de
Release Date : 2014-04-11
The Influence Of Hyproxia An Gata 1 And Epo Expression Levels In Developing Zebrafish written by Markus Holotta and has been published by diplom.de this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-11 with Science categories.
Inhaltsangabe:Abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticeable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development. The zebrafish Danio rerio, also known as Brachidanio rerio, Cyprinus rerio and others, is a omnivorous, tropical fish of the family Cyprinidae and was at fist described by Hamilton in 1822. Its natural range within Asia are slow moving or stagnant water bodies in India, Bangladesh, Nepal and Pakistan. In the past few decades the zebrafish became an important model organism for genetical, developmental and physiological studies. Fish are vertebrates and thus the genetic program is more similar to that of mammals than invertebrate models like the fruity (Drosophila melanogaster). Because of this relationship most of the zebrafish genes have human orthologs. Due to its short generation time of approximately 3 month, the rapid development and the transparency up to adulthood this tropical teleost is predestined for in vivo hematologic studies and digital imaging techniques. Especially digital imaging is a gentle, non-invasive and thereby seminal method for researcher who are working with transparent animals. The combination with fluorescent reporter genes allows real time imaging of gene expression and cell migration studies over their whole lifespan. Inhaltsverzeichnis:Table of Contents: Abbreviations4 Abstract5 1.Introduction6 1.1Zebra_sh hematopoiesis7 1.2GATA-1 and Co-factors8 1.2.1The role of GATA-18 1.2.2GATA-1 related factors10 1.3Epo and EpoR11 1.4Oxygen [...]
The Influence Of Hypoxia On Gata 1 And Epo Expression Levels In Developing Zebrafish
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Author : Markus Holotta
language : en
Publisher: GRIN Verlag
Release Date : 2008
The Influence Of Hypoxia On Gata 1 And Epo Expression Levels In Developing Zebrafish written by Markus Holotta and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.
Master's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), 90 entries in the bibliography, language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.
Role Of Oxidative Stress In Chronic Diseases
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Author : Isaias Dichi
language : en
Publisher: CRC Press
Release Date : 2014-03-14
Role Of Oxidative Stress In Chronic Diseases written by Isaias Dichi and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-03-14 with Medical categories.
This book presents recent findings on the role of oxidative stress in chronic diseases. Understanding the mechanisms behind it enables readers to comprehend the rationale which underlies intervention in such conditions. The book places special emphasis on genetic polymorphism-an important issue related to this field of study. It covers the role of
Angiogenesis Assays
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Author : Carolyn A. Staton
language : en
Publisher: John Wiley & Sons
Release Date : 2007-01-11
Angiogenesis Assays written by Carolyn A. Staton and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-11 with Medical categories.
Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.
Functional Genomics In Aquaculture
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Author : Marco Saroglia
language : en
Publisher: John Wiley & Sons
Release Date : 2012-05-25
Functional Genomics In Aquaculture written by Marco Saroglia and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-25 with Technology & Engineering categories.
Genomics has revolutionized biological research over the course of the last two decades. Genome maps of key agricultural species have offered increased understanding of the structure, organization, and evolution of animal genomes. Building upon this foundation, researchers are now emphasizing research on genome function. Published with the World Aquaculture Society, Functional Genomics in Aquaculture looks at the advances in this field as they directly relate to key traits and species in aquaculture production. Functional Genomics in Aquaculture opens with two chapters that provide a useful general introduction to the field of functional genomics. The second section of the book focuses on key production traits such as growth, development, reproduction, nutrition, and physiological response to stress and diseases. The final five chapters focus on a variety of key aquaculture species. Examples looking at our understanding of the functional genomes of salmonids, Mediterranean sea bass, Atlantic cod, catfish, shrimp, and molluscs, are included in the book. Providing valuable insights and discoveries into the functional genomes of finfish and shellfish species, Functional Genomics in Aquaculture, will be an invaluable resource to researchers and professionals in aquaculture, genetics, and animal science.
Oxygen Sensing
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Author :
language : en
Publisher: Elsevier
Release Date : 2004-05-10
Oxygen Sensing written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-10 with Science categories.
The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields.
The Biology Of Caves And Other Subterranean Habitats
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Author : David C. Culver
language : en
Publisher: Oxford University Press
Release Date : 2019-04-01
The Biology Of Caves And Other Subterranean Habitats written by David C. Culver and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-01 with Science categories.
The second edition of this widely cited textbook continues to provide a concise but comprehensive introduction to cave and subterranean biology, describing this fascinating habitat and its biodiversity. It covers a range of biological processes including ecosystem function, evolution and adaptation, community ecology, biogeography, and conservation. The authors draw on a global range of examples and case studies from both caves and non-cave subterranean habitats. One of the barriers to the study of subterranean biology has been the extraordinarily large number of specialized terms used by researchers; the authors explain these terms clearly and minimize the number that they use. This new edition retains the same 10 chapter structure of the original, but the content has been thoroughly revised and updated throughout to reflect the huge increase in publications concerning subterranean biology over the last decade.
Gene Therapy
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Author : Mauro Giacca
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-11-01
Gene Therapy written by Mauro Giacca and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-01 with Medical categories.
I entered the gene therapy field in the mid-1990s, being fascinated by the immense potential of genes as drugs for the treatment of human disease. Since then, I have experienced the ups and downs of this discipline, and tried to contribute with my work and that of my laboratory to the development of innovative approaches to the treatment of cardiovascular disorders. During these years, I have had several opp- tunities to speak on gene therapy at lectures and academic lessons, and have often noticed that the field is very attractive to scientists of all disciplines. However, as yet no comprehensive book on the subject has been published. Indeed, most books in the field are either a collection of gene transfer laboratory protocols or deal with the subject in a rather superficial manner. Hence the idea to write a gene therapy textbook that is broad and comprehensive, but at the same time provides sufficient molecular and clinical detail to be of interest to students, professors, and specialists in the various disciplines that contribute to gene therapy. I have tried to keep the language plain and, whenever possible, non-technical. Since the book is intended to be a textbook in the field of gene therapy in both the basic science and clinical areas, whenever technical descriptions are required, they are provided.
Molecular Mechanism Of Congenital Heart Disease And Pulmonary Hypertension
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Author : Toshio Nakanishi
language : en
Publisher: Springer Nature
Release Date : 2020-02-28
Molecular Mechanism Of Congenital Heart Disease And Pulmonary Hypertension written by Toshio Nakanishi and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-02-28 with Medical categories.
This open access book focuses on the molecular mechanism of congenital heart disease and pulmonary hypertension, offering new insights into the development of pulmonary circulation and the ductus arteriosus. It describes in detail the molecular mechanisms involved in the development and morphogenesis of the heart, lungs and ductus arteriosus, covering a range of topics such as gene functions, growth factors, transcription factors and cellular interactions, as well as stem cell engineering technologies. The book also presents recent advances in our understanding of the molecular mechanism of lung development, pulmonary hypertension and molecular regulation of the ductus arteriosus. As such, it is an ideal resource for physicians, scientists and investigators interested in the latest findings on the origins of congenital heart disease and potential future therapies involving pulmonary circulation/hypertension and the ductus arteriosus.
Zebrafish
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Author : James F. Amatruda
language : en
Publisher: Springer Nature
Release Date : 2023-09-05
Zebrafish written by James F. Amatruda and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-05 with Science categories.
This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.