Two Phase Systems
DOWNLOAD
Download Two Phase Systems PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Two Phase Systems book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Aqueous Two Phase Systems
DOWNLOAD
Author : Rajni Hatti-Kaul
language : en
Publisher:
Release Date : 2000
Aqueous Two Phase Systems written by Rajni Hatti-Kaul and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.
Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
Aqueous Two Phase Systems
DOWNLOAD
Author : Rajni Hatti-Kaul
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Aqueous Two Phase Systems written by Rajni Hatti-Kaul and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products
DOWNLOAD
Author : Marco Rito-Palomares
language : en
Publisher: Springer
Release Date : 2017-07-18
Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products written by Marco Rito-Palomares and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-18 with Technology & Engineering categories.
This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
Partitioning In Aqueous Two Phase System
DOWNLOAD
Author : Harry Walter
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Partitioning In Aqueous Two Phase System written by Harry Walter and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
The Science And Application Of Aqueous Two Phase Systems And Liquid Liquid Phase Separation In Biotechnology And Bioengineering
DOWNLOAD
Author : John Paul Frampton
language : en
Publisher: Frontiers Media SA
Release Date : 2020-01-13
The Science And Application Of Aqueous Two Phase Systems And Liquid Liquid Phase Separation In Biotechnology And Bioengineering written by John Paul Frampton and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-13 with categories.
The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.
Two Phase Flow In Complex Systems
DOWNLOAD
Author : Salomon Levy
language : en
Publisher: John Wiley & Sons
Release Date : 1999-08-02
Two Phase Flow In Complex Systems written by Salomon Levy and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-08-02 with Technology & Engineering categories.
Mehrphasige Strömungen spielen in etlichen Industriezweigen, besonders der Luft- und Raumfahrt und der Energieerzeugung, eine zentrale Rolle. Derart komplexe Strömungsvorgänge sind extrem schwer vorauszuberechnen, zu analysieren und zu testen. Wertvolle Hilfestellungen, die für reale technische Situationen gedacht sind, gibt dieser Band, der auch Quelltexte einschlägiger Computerprogramme enthält. (07/99)
Aqueous Two Phase Systems
DOWNLOAD
Author : Vangelis Anatolijs Xanthopoulos
language : en
Publisher:
Release Date : 2018
Aqueous Two Phase Systems written by Vangelis Anatolijs Xanthopoulos and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Phase partition categories.
Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids. In Aqueous Two-Phase Systems: Properties, Functions and Advantages, the authors begin by discussing the benefits of these systems.The following chapter presents the main forms of the purification of lipases from aqueous biphasic systems, addressing and discussing the fundamentals for the formation, use and optimization of these systems applied in the biotechnological context. The authors address the basis of aqueous-biphasic systems, aqueous two-phase systems for enzymatic purification, purification of lipases and combined uses of aqueous two-phase systems.The most important experimental parameters that affect the efficiency of metal ions extraction in aqueous PEG-based two-phase systems are examined to provide a starting point in the design of a suitable system for the extraction of metal ions. Compared to the conventional solvent extraction, which uses toxic, flammable and volatile organic solvents and can be quite expensive, the extraction in aqueous PEG-based two-phase systems is considered a more environmental friendly and economically viable method.Also in this compilation, an overview about application of ATPSs in microstructured devices is provided. Microstructured devices offer potential benefits due to well-defined high specific interfacial areas available for heat and mass transfer. These areas increase transfer rate and enhance yield, selectivity and process control. The application of ATPSs in Micro Total Analysis Systems is described as well.
Aqueous Two Phase Systems
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 1994
Aqueous Two Phase Systems written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.
Transport Processes In Boiling And Two Phase Systems Including Near Critical Fluids
DOWNLOAD
Author : Yih-Yun Hsu
language : en
Publisher:
Release Date : 1986
Transport Processes In Boiling And Two Phase Systems Including Near Critical Fluids written by Yih-Yun Hsu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Science categories.
This book presents concise views of current theories on boiling and two-phase flow and on supercritical heat transfer. The mechanisms of these two modes of heat transfer are compared and contrasted. The theories propose to interpret the observed phenomena from a mechanistic viewpoint, with supporting correlations and equations. Each subsection includes a summary and reference list, and nomenclatures are provided for each major section.
Cryogenic Two Phase Flow
DOWNLOAD
Author : N. N. Filina
language : en
Publisher: Cambridge University Press
Release Date : 1996-05-31
Cryogenic Two Phase Flow written by N. N. Filina and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-05-31 with Science categories.
Cryogenic systems that involve two-phase (vapor-liquid) flows are widely used in industries such as aerospace, metallurgy, power engineering, and food production, as well as in high energy physics research. The purpose of this book is to describe characteristic features of cryogenic systems involving two-phase flow, create mathematical models of these systems, and then show how the models may be used to develop optimal designs for practical cryogenic systems. The models are examined using analytical and numerical techniques, and then the predictions are compared to experimental measurements. Since transient phenomena can produce severe and unexpected effects in cryogenic systems, the authors pay particular attention to this important topic. Examples in the book are drawn from cryogenic fluid transport, gasification, and the stabilization of superconducting magnets. Much of this work is related to the development of large Russian systems in the areas of space technology, energy research, and particle physics. This book, the first devoted solely to cryogenic two-phase flow, will be a valuable reference for cryogenic engineers and scientists.