Aqueous Two Phase Systems
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Aqueous Two Phase Systems
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Author : Rajni Hatti-Kaul
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Aqueous Two Phase Systems written by Rajni Hatti-Kaul and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Aqueous Two Phase Systems
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Author : Rajni Hatti-Kaul
language : en
Publisher:
Release Date : 2000
Aqueous Two Phase Systems written by Rajni Hatti-Kaul and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.
Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
Partitioning In Aqueous Two Phase System
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Author : Harry Walter
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Partitioning In Aqueous Two Phase System written by Harry Walter and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products
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Author : Marco Rito-Palomares
language : en
Publisher: Springer
Release Date : 2017-07-18
Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products written by Marco Rito-Palomares and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-18 with Technology & Engineering categories.
This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
The Science And Application Of Aqueous Two Phase Systems And Liquid Liquid Phase Separation In Biotechnology And Bioengineering
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Author : John Paul Frampton
language : en
Publisher: Frontiers Media SA
Release Date : 2020-01-13
The Science And Application Of Aqueous Two Phase Systems And Liquid Liquid Phase Separation In Biotechnology And Bioengineering written by John Paul Frampton and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-13 with categories.
The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.
Aqueous Two Phase Systems
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Author : Vangelis Anatolijs Xanthopoulos
language : en
Publisher:
Release Date : 2018
Aqueous Two Phase Systems written by Vangelis Anatolijs Xanthopoulos and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Phase partition categories.
Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids. In Aqueous Two-Phase Systems: Properties, Functions and Advantages, the authors begin by discussing the benefits of these systems.The following chapter presents the main forms of the purification of lipases from aqueous biphasic systems, addressing and discussing the fundamentals for the formation, use and optimization of these systems applied in the biotechnological context. The authors address the basis of aqueous-biphasic systems, aqueous two-phase systems for enzymatic purification, purification of lipases and combined uses of aqueous two-phase systems.The most important experimental parameters that affect the efficiency of metal ions extraction in aqueous PEG-based two-phase systems are examined to provide a starting point in the design of a suitable system for the extraction of metal ions. Compared to the conventional solvent extraction, which uses toxic, flammable and volatile organic solvents and can be quite expensive, the extraction in aqueous PEG-based two-phase systems is considered a more environmental friendly and economically viable method.Also in this compilation, an overview about application of ATPSs in microstructured devices is provided. Microstructured devices offer potential benefits due to well-defined high specific interfacial areas available for heat and mass transfer. These areas increase transfer rate and enhance yield, selectivity and process control. The application of ATPSs in Micro Total Analysis Systems is described as well.
Aqueous Two Phase Systems
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Author : Harry Walter
language : en
Publisher: Elsevier
Release Date : 1994-04-18
Aqueous Two Phase Systems written by Harry Walter and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-04-18 with Nature categories.
General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Aqueous Two Phase Partitioning
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Author : Boris Y. Zaslavsky
language : en
Publisher: CRC Press
Release Date : 1994-11-15
Aqueous Two Phase Partitioning written by Boris Y. Zaslavsky and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-11-15 with Science categories.
Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
Modeling Of Phase Separation In Aqueous Two Phase Systems Containing Salts
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Author : Paresh Umakant Kenkare
language : en
Publisher:
Release Date : 1995
Modeling Of Phase Separation In Aqueous Two Phase Systems Containing Salts written by Paresh Umakant Kenkare and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
Protein Structure Analysis
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Author : Roza Maria Kamp
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Protein Structure Analysis written by Roza Maria Kamp and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.