Aqueous Two Phase Partitioning
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Aqueous Two Phase Systems
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Author : Rajni Hatti-Kaul
language : en
Publisher:
Release Date : 2000
Aqueous Two Phase Systems written by Rajni Hatti-Kaul and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.
Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
Partitioning In Aqueous Two Phase System
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Author : Harry Walter
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Partitioning In Aqueous Two Phase System written by Harry Walter and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Aqueous Two Phase Partitioning
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Author : Boris Y. Zaslavsky
language : en
Publisher: CRC Press
Release Date : 1994-11-15
Aqueous Two Phase Partitioning written by Boris Y. Zaslavsky and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-11-15 with Science categories.
Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
Ionic Liquid Based Aqueous Biphasic Systems
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Author : Mara G. Freire
language : en
Publisher: Springer
Release Date : 2016-10-05
Ionic Liquid Based Aqueous Biphasic Systems written by Mara G. Freire and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Technology & Engineering categories.
This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.
Three Phase Partitioning
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Author : Munishwar Nath Gupta
language : en
Publisher: Elsevier
Release Date : 2021-08-11
Three Phase Partitioning written by Munishwar Nath Gupta and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-11 with Science categories.
Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. - Written by researchers who have been pioneers in developing and utilizing three phase partitioning - Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples - Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols
Separation Processes In Biotechnology
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Author : Juan A. Asenjo
language : en
Publisher: CRC Press
Release Date : 1990-06-28
Separation Processes In Biotechnology written by Juan A. Asenjo and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-06-28 with Science categories.
Edited to avoid duplication and favor comprehensiveness, 20 contributors detail the recovery, separation, and purification operations of bioprocess technology. Individual chapters in this classic yet still highly relevant work emphasize concepts that are becoming more and more important when applied to the large scale versions of techniques that are considered well established. Aside from fully discussing processes, Separation Processes in Biotechnology includes sections on concentration separation and operation, purification operations, and product release and recovery. It also discusses plant operation and equipment and delves into economic considerations
Aqueous Two Phase Systems
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Author : Rajni Hatti-Kaul
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Aqueous Two Phase Systems written by Rajni Hatti-Kaul and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Handbook Of Bioseparations
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Author : Satinder Ahuja
language : en
Publisher: Elsevier
Release Date : 2000-06-23
Handbook Of Bioseparations written by Satinder Ahuja and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-06-23 with Technology & Engineering categories.
It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories.Key Features* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies* Encompasses both analytical and process-scale methods* Elucidates the importance of engineering process control* Details selection of plant and process equipment* Addresses economic considerations* Discusses future developments
Partitioning In Aqueous Two Phase Systems
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Author : Harry Walter
language : en
Publisher: Orlando ; Toronto : Academic Press
Release Date : 1985
Partitioning In Aqueous Two Phase Systems written by Harry Walter and has been published by Orlando ; Toronto : Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Science categories.
Partitioning In Aqueous Two - Phase System ...
Microcompartmentation And Phase Separation In Cytoplasm
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Author :
language : en
Publisher: Academic Press
Release Date : 1999-10-21
Microcompartmentation And Phase Separation In Cytoplasm written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-10-21 with Science categories.
International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.This volume provides an overview of major cytoplasmic properties and events which including cytoarchitecture and the physical properties of cytoplasm, molecular compartmentation and gradients, channeling, sorting, and trafficking. It also addresses physicochemical events, both measured and anticipated, which attend solutions under conditions prevailing in cytoplasm: molecular crowding. It summarizes the current state of knowledge in the field and considers questions such as how molecules in cytoplasm interact.