Whole Cell Biocatalysis
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Novel Enzyme And Whole Cell Biocatalysts
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Author : Anwar Sunna
language : en
Publisher: MDPI
Release Date : 2020-11-04
Novel Enzyme And Whole Cell Biocatalysts written by Anwar Sunna and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-04 with Science categories.
The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
Enzyme Or Whole Cell Immobilization For Efficient Biocatalysis Focusing On Novel Supporting Platforms And Immobilization Techniques
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Author : Wen-Yong Lou
language : en
Publisher: Frontiers Media SA
Release Date : 2021-04-21
Enzyme Or Whole Cell Immobilization For Efficient Biocatalysis Focusing On Novel Supporting Platforms And Immobilization Techniques written by Wen-Yong Lou and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-21 with Science categories.
Whole Cell Biocatalysis
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Author : Sudheer D. V N. Pamidimarri
language : en
Publisher:
Release Date : 2023-12
Whole Cell Biocatalysis written by Sudheer D. V N. Pamidimarri and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12 with Science categories.
Whole Cell Biocatalysis In The Presence Of Supercritical And Compressed Solvents
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Author : Jason A. Berberich
language : en
Publisher:
Release Date : 2001
Whole Cell Biocatalysis In The Presence Of Supercritical And Compressed Solvents written by Jason A. Berberich and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Catalysis categories.
Novel Enzyme And Whole Cell Biocatalysts
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Author : Anwar Sunna
language : en
Publisher:
Release Date : 2020
Novel Enzyme And Whole Cell Biocatalysts written by Anwar Sunna and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with categories.
The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
In Situ Removal Of Solid Products Whole Cell Biocatalysis
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Author : Evelyn Montajes Buque
language : en
Publisher:
Release Date : 2005
In Situ Removal Of Solid Products Whole Cell Biocatalysis written by Evelyn Montajes Buque and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with categories.
Accelerating Whole Cell Biocatalysis By Enhancing Outer Membrane Permeability
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Author : Ye Ni
language : en
Publisher:
Release Date : 2006
Accelerating Whole Cell Biocatalysis By Enhancing Outer Membrane Permeability written by Ye Ni and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Cytology categories.
Whole-cell biocatalysts are preferred in many biocatalysis applications. However, cell envelope often represents a formidable permeability barrier. As a result, reactions catalyzed by whole-cells are reportedly orders of magnitude slower than those of by their free enzyme counterparts. The present research addresses this critical issue by using membrane engineering approaches. Two E. coli strains with genetically altered outer membrane structures were used in the study, a lipopolysaccarides (LPS) mutant SM101 and a Braun's lipoprotein mutant E609L. The effects of outer membrane mutation on the permeability of substrates differing substantially in size and hydrophobicity were investigated by combining the mutant cells with model enzymes. The reduction of the outer membrane permeability barrier by these mutations led to significant accelerations (2 to 14 fold) in reaction rates of all whole-cell catalyzed reactions investigated. In the case of tetrapeptide, LPS mutation of the outer membrane can render the outer membrane completely permeable to substrate, a barrier-less condition that maximizes the reaction rate. For reaction rates of toluene dioxygenase (TDO)-catalyzed reactions, a dramatic increase of up to six fold was observed with the lipoprotein mutant for each of the three small, hydrophobic substrates tested. Mutations in either the LPS or in the Braun's lipoprotein are effective for accelerating reactions with UDP-glucose, resulting in a striking acceleration (up to 14 fold) of reaction rate. The magnitude of reaction rate acceleration was found to be dependent upon the substrate concentrations, the enzyme expression level, and on the nature of the mutations and substrates. In addition, the mutations have been demonstrated to be far more superior to common permeabilization procedures like freeze-thaw (FT) or treatment with the chelating agent EDTA (ethylene diamine tetraacetic acid). Importantly, lipoprotein mutant E609L exhibited a normal growth rate and expressed the recombinant multi-component enzyme as well as the isogenic parent. The exact nature of lpp lipoprotein mutation in E609L was further studied and deletion of lpp was successfully introduced into E. coli strain with different genetic background for whole-cell biocatalysis applications. An example was provided by introducing an lpp deletion into an E. coli O44K74 strain to achieve a higher yield for L-carnitine production. This research and the results outlined in this dissertation demonstrate a valid strategy for addressing permeability issues in whole-cell biocatalysis. The work also highlights a need for accessing substrate permeabilities in biocatalysis research and development.
Practical Methods For Biocatalysis And Biotransformations 2
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Author : John Whittall
language : en
Publisher: John Wiley & Sons
Release Date : 2012-04-16
Practical Methods For Biocatalysis And Biotransformations 2 written by John Whittall and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-16 with Technology & Engineering categories.
Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a "how-to" guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume – which can be used on its own or in combination with the first volume - concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.
Microbial Biocatalysis
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Author : Zhilong Wang
language : en
Publisher: Mdpi AG
Release Date : 2023-04-06
Microbial Biocatalysis written by Zhilong Wang and has been published by Mdpi AG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-06 with Technology & Engineering categories.
Biocatalysis is a sustainable alternative for the chemical industry in manufacturing, monitoring, and waste management. Biocatalytic processes perform with isolated enzymes or whole cells as biocatalysts. Whole-cell biocatalysts offer some unique advantages of cascade reactions catalyzed by multienzymes as well as a single bioredox reaction with cofactor regeneration in a single strain. Therefore, whole-cell biocatalysts are widely applied for biosynthesis/biotransformation to produce value-added chemicals as well as the complete mineralization of organic pollutants. Biological catalytic processing using whole-cell biocatalysts includes biocatalyst engineering, bio-reaction engineering, and downstream processing. In addition to the traditional screening of microbial strains and immobilized whole-cell biocatalysts, modern genetic engineering, metabolic engineering, and synthetic biology make tailored whole-cell biocatalysts possible. At the same time, some integrated processes have successfully been applied in the catalytic processing using living whole-cell biocatalysts, such as harnessing biocompatible chemistry to interface with the microbial metabolism as well as using various separation techniques for in situ product removal. This reprint on "Microbial Biocatalysis" provides a comprehensive overview of the recent developments of catalyst discovery, catalyst modification, and process intensification for whole cell catalysis in fermentation, biotransformation or biodegradation processes.
Use Of Surfactants In The Whole Cell Biocatalysis Of Sparingly Soluble Substrates
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Author : Sowmya Talari
language : en
Publisher:
Release Date : 2004
Use Of Surfactants In The Whole Cell Biocatalysis Of Sparingly Soluble Substrates written by Sowmya Talari and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Catalysis categories.
