A System For Production Of Pure Influenza A Virus Defective Interfering Particles Dips And Assessment Of Their Antiviral Activity

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A System For Production Of Pure Influenza A Virus Defective Interfering Particles Dips And Assessment Of Their Antiviral Activity

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Author : Najat Khodor Bdeir
language : en
Publisher:
Release Date : 2021

A System For Production Of Pure Influenza A Virus Defective Interfering Particles Dips And Assessment Of Their Antiviral Activity written by Najat Khodor Bdeir and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.

Influenza A virus (IAV) epidemics and pandemics constitute a major threat to human health.The ability of IAV to rapidly acquire mutations can render antivirals and vaccines ineffective andnovel antiviral strategies are urgently needed. IAV particles harbor eight genomic RNA segmentsand defective interfering RNAs (DI RNAs) are naturally occurring byproducts of viral genomereplication. These RNAs interfere with replication of the WT RNAs and, upon packaging intodefective interfering particles (DIPs), can inhibit spread of WT IAV. It has been postulated thatDIPs interfere with IAV infection by...

A System For Production Of Pure Influenza A Virus Defective Interfering Particles Dips And Assessment Of Their Antiviral Activity

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Author : Najat Bdeir
language : en
Publisher:
Release Date : 2021

A System For Production Of Pure Influenza A Virus Defective Interfering Particles Dips And Assessment Of Their Antiviral Activity written by Najat Bdeir and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.

Influenza A virus (IAV) epidemics and pandemics constitute a major threat to human health.The ability of IAV to rapidly acquire mutations can render antivirals and vaccines ineffective andnovel antiviral strategies are urgently needed. IAV particles harbor eight genomic RNA segmentsand defective interfering RNAs (DI RNAs) are naturally occurring byproducts of viral genomereplication. These RNAs interfere with replication of the WT RNAs and, upon packaging intodefective interfering particles (DIPs), can inhibit spread of WT IAV. It has been postulated thatDIPs interfere with IAV infection by...

Quantification And Integration Of The Multi Scale Effects Of Defective Interfering Particles On Virus Infections

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Author :
language : en
Publisher:
Release Date : 2014

Quantification And Integration Of The Multi Scale Effects Of Defective Interfering Particles On Virus Infections written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.

Defective interfering particles (DIPs) are virus mutants that lack essential genes for growth, but able to divert the viral proteins produced by an infectious virus, and thus, interfere with infectious virus production. The accumulating evidence about their presence in nature and the increasing interest in DIP-based vaccine formulations highlight the need for an accurate understanding of the effect of DIPs on virus growth at intracellular and cell-to-cell spread scale. To analyze the multi-scale effects of DIPs, we first tracked the intracellular growth of a recombinant vesicular stomatitis virus (VSV) in individual cells using fluorescence microscopy and quantified the effects of DIPs on intracellular virus production kinetics. Through a metric scoring the dose-dependent effects of DIPs in cells and their cell-to-cell variations, we identified two-fold higher degree of interference by DIPs in isolated cells than that in single cells within a population. Quantification of infection spread behavior revealed an induction in the diversity of spread patterns with increasing DIP input. Through a cellular automata model driven by the measured virus growth kinetics in individual cells, we found that the intracellular scale effects of DIPs and cell-to-cell variations play a major role in the formation of the diverse interfered spread patterns. Using the developed framework the DIP-induced interference was compared to the interference by a recombinant defective viral mutant that only lacks virus assembly proteins. Our findings suggested that during the DIP co-infections the competition at replication stage interferes mainly with the early growth delaying and reducing the intracellular virus activity, and thus decreasing extracellular virus production, which is further diminished by the competition at virus assembly stage. Despite this reduction in virus production, we observed that the enrichment of DIPs are limited by the growth of infectious virus suggesting the dependency of DIPs on infectious virus at all scales of virus infections. Overall, the insight provided by this work can guide future studies of the viral infectious disease progression in the presence of DIPs, while the developed framework sets a platform to investigate other microbial infections under the effects of different interfering substances, such as antiviral or antibacterial drugs and vaccines.

Animal Cell Biotechnology

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Author : Ralf Pörtner
language : en
Publisher: Humana
Release Date : 2016-08-23

Animal Cell Biotechnology written by Ralf Pörtner and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Bibliography Of Agriculture

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Author :
language : en
Publisher:
Release Date : 1992-02

Bibliography Of Agriculture written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-02 with Agriculture categories.

Bibliography Of Agriculture With Subject Index

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Author :
language : en
Publisher:
Release Date : 1992

Bibliography Of Agriculture With Subject Index written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Agriculture categories.

Principles Of Food Sanitation

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Author : Norman G. Marriott
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09

Principles Of Food Sanitation written by Norman G. Marriott and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Technology & Engineering categories.

Large volume food processing and preparation operations have increased the need for improved sanitary practices from processing to consumption. This trend presents a challenge to every employee in the food processing and food prepara tion industry. Sanitation is an applied science for the attainment of hygienic conditions. Because of increased emphasis on food safety, sanitation is receiving increased attention from those in the food industry. Traditionally, inexperienced employees with few skills who have received little or no training have been delegated sanitation duties. Yet sanitation employees require intensive training. In the past, these employees, including sanitation program managers, have had only limited access to material on this subject. Technical information has been confined primarily to a limited number of training manuals provided by regulatory agen cies, industry and association manuals, and recommendations from equipment and cleaning compound firms. Most of this material lacks specific information related to the selection of appropriate cleaning methods, equipment, compounds, and sanitizers for maintaining hygienic conditions in food processing and prepara tion facilities. The purpose of this text is to provide sanitation information needed to ensure hygienic practices. Sanitation is a broad subject; thus, principles related to con tamination, cleaning compounds, sanitizers, and cleaning equipment, and specific directions for applying these principles to attain hygienic conditions in food processing and food preparation are discussed. The discussion starts with the importance of sanitation and also includes regulatory requirements and voluntary sanitation programs including additional and updated information on Hazard Analysis Critical Control Points (HACCP).

Pathology Illustrated

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Author : Alasdair D. T. Govan
language : en
Publisher:
Release Date : 1996

Pathology Illustrated written by Alasdair D. T. Govan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Pathology categories.

Pathology Illustrated presents both general and systematic pathology in a highly visual style. This format makes the essential information more accessible and memorable.

New Frontiers Of Molecular Epidemiology Of Infectious Diseases

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Author : Serge Morand
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-09-08

New Frontiers Of Molecular Epidemiology Of Infectious Diseases written by Serge Morand and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-08 with Medical categories.

Molecular epidemiology has recently broaden its focuses due to the development of molecular tools but also by incorporating advances of other fields such as mathematical epidemiology, molecular ecology, population genetics and evolution. Facing new risks of emerging and re-emerging infectious diseases that are threats for humans and their livestock, the objectives of molecular epidemiology include: - the development of molecular tools, genotyping and gene expression - the incorporation of concepts and results of population genetics of infectious diseases - the integration of recent advances in theoretical epidemiology and evolutionary ecology of diseases - a better understanding of transmission for the development of risk factors analyses. This book will demonstrate how the latest developments in molecular tools and in epidemiology can be integrated with studies of host-pathogen interactions. Besides a strong theoretical component, there will also be an emphasis on applications in the fields of epidemiology, public health, veterinary medicine, and health ecology. Students and researchers in the fields of epidemiology, animal and human health, evolutionary ecology, parasitology are the main potential readers of the book, as well as a broader audience from veterinary medicine and conservation.

Current Topics In Microbiology And Immunology

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Author : W. Arber
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Current Topics In Microbiology And Immunology written by W. Arber and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.