Adenovirus Methods And Protocols
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Adenovirus Methods And Protocols
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Author : William S. M. Wold
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Adenovirus Methods And Protocols written by William S. M. Wold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.
Adenovirus
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Author : Miguel Chillón
language : en
Publisher: Humana Press
Release Date : 2013-10-17
Adenovirus written by Miguel Chillón and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-17 with Science categories.
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.
Adenovirus Methods And Protocols
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Author : William S. M. Wold
language : en
Publisher: Humana Press
Release Date : 2007-09-24
Adenovirus Methods And Protocols written by William S. M. Wold and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-24 with Medical categories.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.
Adenoviral Vectors For Gene Therapy
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Author : David T. Curiel
language : en
Publisher: Academic Press
Release Date : 2002-05
Adenoviral Vectors For Gene Therapy written by David T. Curiel and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-05 with Medical categories.
Adenoviral Vectors for Gene Therapy provides detailed and comprehensive coverage of these important therapeutic agents. The topics covered in this book range from the basic biology of adenoviruses, through the construction and purification of adenoviral vectors, cutting-edge vectorology and the use of adenoviral vectors in preclinical animal models, to regulatory issues which must be considered prior to the initiation of human clinical gene therapy trials. The broad scope of this unique volume provides the reader with a complete understanding of the development and use of adenoviral vectors. Key Features * Provides complete coverage of basic biology of adenoviruses, as well as the construction, propagation and purification of adenoviral vectors * Introduces common strategies for the development of adenoviral vectors along with cutting-edge methods for their improvement * Demonstrates noninvasive imaging of adenovirus-mediated gene transfer * Discusses utility of adenoviral vectors in animal disease models * Considers Federal Drug Administration regulations for human clinical trials
Transgenic Mouse Methods And Protocols
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Author : Marten H. Hofker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Transgenic Mouse Methods And Protocols written by Marten H. Hofker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.
Cystic Fibrosis Methods And Protocols
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Author : William R. Skach
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Cystic Fibrosis Methods And Protocols written by William R. Skach and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
Since the cloning of the cystic fibrosis transmembrane conductance re- lator (CFTR) nearly a decade ago, cystic fibrosis (CF) research has witnessed a dramatic expansion into new scientific areas. Basic researchers, clinicians, and patients increasingly rely on fundamental techniques of genetics, molecular biology, electrophysiology, biochemistry, cell biology, microbiology, and immunology to understand the molecular basis of this complex disease. Research into the pathophysiology of CF has established numerous paradigms of ion channel dysfunction that extend from inflammation and infection in the airways of patients to basic mechanisms of protein processing and regulation in intracellular components. With these rapid advances has come an increasing need for research scientists to understand and utilize a growing array of basic laboratory tools. This volume of Methods in Molecular Medicine, Cystic Fibrosis Methods and Protocols satisfies that need by providing detailed protocols for the laboratory techniques used throughout CF research. From electrophysiology and cell biology, to animal models and gene therapy, the comprehensive set of methods covered here provide step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Contributions have been chosen to reflect the rich diversity of techniques and to provide a cohesive framework for understanding challenges that are currently at the forefront of CF research. It is hoped that this volume will serve as a valuable reference that will not only foster interdisciplinary investigations into current problems encountered in CF, but also facilitate the translation of new scientific discoveries into clinical solutions.
Adenovirus Methods And Protocols Adenoviruses Ad Vectors Quantitation And Animal Models
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Author : William S. M Wold
language : en
Publisher:
Release Date : 2007
Adenovirus Methods And Protocols Adenoviruses Ad Vectors Quantitation And Animal Models written by William S. M Wold and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Adenoviruses categories.
New Antibiotic Targets
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Author : W. Scott Champney
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-01-15
New Antibiotic Targets written by W. Scott Champney and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-15 with Medical categories.
This book examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies. The book will provide laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.
Adenovirus Methods And Protocols
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Author : William S. M. Wold
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Adenovirus Methods And Protocols written by William S. M. Wold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Science categories.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Adenoviruses Model And Vectors In Virus Host Interactions
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Author : Walter Doerfler
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Adenoviruses Model And Vectors In Virus Host Interactions written by Walter Doerfler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.
After three volumes on adenoviruses in 1995 the past years have seen rapid progress in the field of adenovirus research. Moreover, adenoviruses have attracted considerable interest as vectors in gene transfer regimens.