Advanced Flow Cytometry Applications In Biological Research
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Advanced Flow Cytometry Applications In Biological Research
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Author : R.C. Sobti
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Advanced Flow Cytometry Applications In Biological Research written by R.C. Sobti and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Medical categories.
Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.
Flow Cytometry
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Author : Andrew Yen
language : en
Publisher: CRC Press
Release Date : 1989-08-31
Flow Cytometry written by Andrew Yen and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-08-31 with Science categories.
The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.
Flow Cytometry Advd Res Clinical Applns
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Author : Andrew Yen
language : en
Publisher: CRC-Press
Release Date : 1989-08-31
Flow Cytometry Advd Res Clinical Applns written by Andrew Yen and has been published by CRC-Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-08-31 with Science categories.
The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.
Flow Cytometry
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Author : Marion G. Macey
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03
Flow Cytometry written by Marion G. Macey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Flow Cytometry
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Author : Andrew Yen
language : en
Publisher: CRC-Press
Release Date : 1989-08-31
Flow Cytometry written by Andrew Yen and has been published by CRC-Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-08-31 with Science categories.
The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.
Flow Cytometry
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Author : Stefanos Papandreou
language : en
Publisher: Nova Science Publishers
Release Date : 2013
Flow Cytometry written by Stefanos Papandreou and has been published by Nova Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Flow cytometry categories.
Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characterisation of T regulatory and immune suppressor cells; multicolor panel design strategies in rare event analysis of dendritic cells and regulatory T cells; advanced characterisation of individual particle morphology light-scattering flow cytometry; and application of flow cytometry in environmental science.
Advanced Optical Flow Cytometry
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Author : Valery V. Tuchin
language : en
Publisher: John Wiley & Sons
Release Date : 2011-03-31
Advanced Optical Flow Cytometry written by Valery V. Tuchin and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-31 with Science categories.
A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.
Flow Cytometry Protocols
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Author : Teresa S. Hawley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flow Cytometry Protocols written by Teresa S. Hawley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).
Applications Of Flow Cytometry In Stem Cell Research And Tissue Regeneration
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Author : Awtar Krishan
language : en
Publisher: John Wiley & Sons
Release Date : 2011-06-09
Applications Of Flow Cytometry In Stem Cell Research And Tissue Regeneration written by Awtar Krishan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-09 with Science categories.
A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
Flow Cytometry
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Author : Ranbir Chander Sobti
language : en
Publisher: Springer
Release Date : 2024-11-23
Flow Cytometry written by Ranbir Chander Sobti and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-11-23 with Science categories.
Flow cytometry is a state-of-the-art technology that is widely used in biological research and clinical diagnostics. It is a high-throughput platform that allows the quantification, differentiation, and functional study of cells or cell like particles in suspension. Various cell components can be targeted with fluorescently labelled antibodies or fluorescent dyes to allow measurement and analysis of the physical, chemical, and biological properties of individual cells within homogenous or heterogeneous populations. Since its commercialization more than five decades ago, flow cytometry has advanced and become an very important analytical tool in translational research. With the advancement of instrument technology, an increasing number of fluorescent dyes, and an expanding range of monoclonal antibodies, the applications of flow cytometry in applied research continue to grow. The use of multiparametric flow cytometry in translational research provides the ability to rapidly identify different cell populations and to simultaneously measure multiple parameters of single cells for efficiently assessing immune status, decrease/increase of specific immune cell populations, cell activation status, etc. of different cells in preclinical and clinical studies. The primary goal of any research work is to take research results from the "bench to-bed and back". The use of flow cytometry in applied research advances the development of new diagnostic tests or drugs for cancer treatment, immune monitoring, etc. that help in patient care. Today, every biological scientist needs to have basic knowledge of flow cytometry in order to utilise this technology properly in their own research and to understand other’s research work. The present book has been designed to give the knowledge of flow cytometry and its applications to the researchers and teachers. It will allow the readers to utilize the technology in an appropriate way in their research work. This book has describing various applications of flow cytometry like cell health monitoring, immunophenotyping, cell sorting, stem cell characterization, micro-vesicle analysis etc.