Advanced Lab Practices In Biochemistry Molecular Biology
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Advanced Lab Practices In Biochemistry Molecular Biology
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Author : Swati Agarwal
language : en
Publisher: I K International Pvt Ltd
Release Date : 2018-08-10
Advanced Lab Practices In Biochemistry Molecular Biology written by Swati Agarwal and has been published by I K International Pvt Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-10 with Medical categories.
This book provides detailed information on various instruments, techniques and experiment protocols of biochemistry and molecular biology. It deals with basic as well as advanced information and in-depth methodology in simple language to help students and professionals to perform experiments with ease. This book not only clears the practical concepts of Biochemistry and Molecular Biology at undergraduate and post-graduation levels, but also helps to pass the Ph.D. course work exam conducted by various universities. This book will develop research aptitude to clear the NET examination. This manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications. It provides a wide spectrum of 14 chapters covering basic as well as advanced techniques and instrumentation, viz., Gas Chromatography (GC), Mass Spectrometry (MS), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) with detailed protocols. Most of the experiments can be easily performed in the laboratory having basic facilities. Historical background, experiment nature, its principle, step-by-step procedure with diagrammatic representation and important precautions are given in the beginning of each experiment.
Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2010-03-04
Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-04 with Medical categories.
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Modern Experimental Biochemistry
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Author : Rodney Boyer
language : en
Publisher: Pearson
Release Date : 2000
Modern Experimental Biochemistry written by Rodney Boyer and has been published by Pearson this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Juvenile Nonfiction categories.
This successful text provides students majoring in biochemistry, chemistry, biology, and related fields with a modern and complete experience in experimental biochemistry. Its unique two-part organization offers flexibility to accommodate various requirements of the course, and allows students to reference detailed theory sections for clarification during labs. Part I, Theory and Experimental Techniques, provides in-depth theoretical discussion organized around important techniques. A valuable reference for instructors and students, it's particularly useful to instructors who prefer to use their own customized experiments. Part II, Experiments, offers optimum flexibility through 15 tested experiments designed to accommodate the capabilities of laboratories and students at most four-year schools. Alternate methods are suggested and labs may be divided into manageable hour segments.
Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2018-04-19
Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-19 with Medical categories.
A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.
Fundamental Laboratory Approaches For Biochemistry And Biotechnology
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Author : Alexander J. Ninfa
language : en
Publisher: John Wiley & Sons
Release Date : 2009-05-26
Fundamental Laboratory Approaches For Biochemistry And Biotechnology written by Alexander J. Ninfa and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-05-26 with Science categories.
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills, allowing students to learn techniques and develop the the critical thinking and organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques but also includes molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The experiments are designed so that theory and technique are learned as fundamental research tools, and the biochemistry and molecular biology applications are seamlessly integrated throughout the manual. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors’ three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual’s experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a “cookbook” approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Lab Ref
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Author : Jane Roskams
language : en
Publisher: CSHL Press
Release Date : 2002
Lab Ref written by Jane Roskams and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
Table of contents: Section 1 Most Commonly Used Solutions A. Stock Solutions, 1 B. Biological Buffers, 13 C. Proteins, Enzymes, and Antibiotics, 27 D. Reagents for the Analysis, Labeling, and Synthesis of Nucleic Acids, 35 Section 2 Macromolecular Preparation and Purification Reagents A. DNA, 43 B. RNA, 47 C. Protein, 53 Section 3 Electrophoretic Separation of Proteins and Nucleic Acids A. Electrophoresis of DNA, RNA, and Protein, 63 B. Transfer, Hybridization, and Screening of DNA, RNA, and Protein, 81 Section 4 Visualizing Genes and Gene Products A. Use of Antibodies for Immunochemical Approaches: A Guide, 95 B. Fixatives, 101 C. Cytological Stains, Chromogen Substrates, and Fluorophores, 105 D. Mounting Media, 119 E. Microscopy Information, 123 Section 5 Specialized Media, Buffers, and Reagents A. Most Commonly Used Bacterial Media and Solutions, 133 B. Yeast, 139 C. Xenopus, 155 D. Mammalian Cell Culture, 161 Section 6 Storage and Shipment of Biological Samples, 169.
Laboratory Protocols In Fungal Biology
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Author : Vijai Kumar Gupta
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-09
Laboratory Protocols In Fungal Biology written by Vijai Kumar Gupta and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-09 with Science categories.
Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology
Introduction To Experimental Biophysics
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Author : Jay L. Nadeau
language : en
Publisher: CRC Press
Release Date : 2016-04-19
Introduction To Experimental Biophysics written by Jay L. Nadeau and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Science categories.
Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often s