Advanced Techniques In Biological Electron Microscopy Ii
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Advanced Techniques In Biological Electron Microscopy Ii
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Author : J. K. Koehler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Advanced Techniques In Biological Electron Microscopy Ii written by J. K. Koehler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The use of the term "advanced" in the title of this book is somewhat ar bitrary and very much relative with respect to time. Many techniques which were considered at the "cutting edge" of ultrastructural methodology just a few years ago are now rou tin ely used in numerous laboratories. One could cite freeze-fracture, cryothin sectioning, or indeed most of the field of scan ning electron microscopy as concrete examples. Thus the use of the term "ad vanced techniques" must be interpreted with regard to the present state of the art, and is useful only in informing the potential reader that this volume is not a primer to be used as an initial introduction into basic biological elec tron microscopy. Many excellent volumes have filled that niche in the past few years, and it is not intended that this modest book be a complete com pendium of the field. Furthermore, any limited selection of papers on advanc ed techniques necessarily reflects the preferences and arbitrary whims of the editor, thereby excluding many equally important procedures which the knowledgeable reader will readily identify. The first volume of this series appeared approximately five years ago and illustrated techniques which were thought to represent advanced and yet ba sically morphological methods for gaining increased ultrastructural informa tion from biological specimens. The present volume, on the other hand, stresses techniques which provide specific physicochemical data on the speci mens in addition to the structural information.
Advanced Techniques In Biological Electron Microscopy
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Author : J.K. Koehler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Advanced Techniques In Biological Electron Microscopy written by J.K. Koehler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Advanced Techniques In Biological Electron Microscopy Iii
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Author : J.K. Koehler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Advanced Techniques In Biological Electron Microscopy Iii written by J.K. Koehler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This volume is a continuation of two prior books on advanced electron microscope techniques. The purpose of this series has been to provide in depth analyses of methods which are considered to be at the leading edge of electron microscopic research procedures with applications in the biological sciences. The mission of the present volume remains that of a source book for the research practitioner or advanced student, especially one already well versed in basic electron optical methods. It is not meant to provide in troductory material, nor can this modest volume hope to cover the entire spectrum of advanced technology now available in electron microscopy. In the past decade, computers have found their way into many research laboratories thanks to the enormous increase in computing power and stor age available at a modest cost. The ultrastructural area has also benefited from this expansion in a number of ways which will be illustrated in this volume. Half of the contributions discuss technologies that either directly or indirectly make extensive use of computer methods.
Advanced Techniques In Biological Electron Microscopy
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Author : James K. Koehler
language : en
Publisher:
Release Date : 1973
Advanced Techniques In Biological Electron Microscopy written by James K. Koehler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with Biology categories.
Biological Electron Microscopy
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Author : Michael J. Dykstra
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-12-31
Biological Electron Microscopy written by Michael J. Dykstra and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-12-31 with Science categories.
Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
Advanced Techniques In Biological Electron Microscopy Ii
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Author : James K. Koehler
language : en
Publisher: Springer
Release Date : 1978
Advanced Techniques In Biological Electron Microscopy Ii written by James K. Koehler and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1978 with Science categories.
Three friends encounter a terrifying experience with a time warp when one falls into a cupboard and becomes an infant.
Advanced Techniques In Biological Electron Microscopy Specific Ultrastructural Probes
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Author : James K. Koehler
language : en
Publisher:
Release Date : 1973
Advanced Techniques In Biological Electron Microscopy Specific Ultrastructural Probes written by James K. Koehler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with Electron microscopy categories.
Electron Tomography
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Author : Joachim Frank
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-05
Electron Tomography written by Joachim Frank and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-05 with Science categories.
This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope. The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.
Biological Field Emission Scanning Electron Microscopy 2 Volume Set
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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-04-29
Biological Field Emission Scanning Electron Microscopy 2 Volume Set written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-29 with Science categories.
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Electron Microscopy
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Author : John J. Bozzola
language : en
Publisher: Jones & Bartlett Learning
Release Date : 1999
Electron Microscopy written by John J. Bozzola and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.
New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR