Advances In The Regulation And Production Of Fungal Enzymes By Transcriptomics Proteomics And Recombinant Strains Design
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Advances In The Regulation And Production Of Fungal Enzymes By Transcriptomics Proteomics And Recombinant Strains Design
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Author : André Damasio
language : en
Publisher: Frontiers Media SA
Release Date : 2019-10-04
Advances In The Regulation And Production Of Fungal Enzymes By Transcriptomics Proteomics And Recombinant Strains Design written by André Damasio and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-04 with categories.
Several efforts have been made in developing strategies to supply the enzyme market, as well as in reducing its costs. It includes the selection of an appropriate enzyme source and the optimization of enzyme properties and secretion. Carbohydrate-Active Enzymes (CAZymes) are industrially relevant biocatalysts that are capable of degrading plant cell wall biomass. The most important secreted enzymes related to plant cell wall decomposition are cellulases, hemicellulases, and auxiliary enzymes. These enzymes have been applied in the hydrolysis of plant biomass for the production of second-generation (2G) ethanol and several other high added value products. One of the bottlenecks for 2G ethanol production is the cost of enzymes applied on plant biomass hydrolysis. The improvement of proteins production by fungi applying system biology and genetic engineering is an interesting and promising strategy to reduce the enzymes cost and make the 2G ethanol production viable. Fungi play an important role in plant biomass degradation and biotechnology by producing and secreting high yields of enzymes. In spite of the fact that filamentous fungi present several advantages compared to other microorganisms due to the high level of proteins production, heterologous protein production is far from optimal levels and still needs to be improved. Currently, heterologous production of certain proteins is generally considerably lower than the levels obtained to homologous production. Many strategies have been studied in order to improve heterologous production of proteins by filamentous fungi, including the deletion of genes that encode for proteases, the deletion of lectin-like ER-Golgi cargo receptors and the co-expression of specific chaperones. It has been shown that the main bottleneck in the production of heterologous proteins is not caused by the low expression of the target gene. An experimental evidence suggests that most target proteins produced in filamentous fungi are lost or stuck in the secretory pathway due to errors in processing, modification or misfolding that result in their elimination by endoplasmic reticulum (ER) quality control. Misfolded proteins alter homeostasis and proper ER functioning resulting in a state known as ER stress. ER stress activates a conserved signaling pathway called unfolded protein response (UPR) and ER-associated protein degradation (ERAD), which upregulates genes responsible for restoring protein folding homeostasis in the cell and degrades misfolded protein in the cytosol by the ubiquitin-proteasome system. The genetic manipulation of individual genes and changes in the genome seems not to be the best alternative to overcome the main bottlenecks in heterologous protein secretion. However, the understanding of complex interactions of important proteins and genes, as well as how they are regulated is more promising.
Plant Mycobiome
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Author : Younes M. Rashad
language : en
Publisher: Springer Nature
Release Date : 2023-04-03
Plant Mycobiome written by Younes M. Rashad and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-03 with Science categories.
Plant mycobiome represents a diverse array of plant-associated communities of endophytic and epiphytic fungi. These communities fundamentally affect plant health, development, adaptation, and communication with the surrounding ecosystem. Furthermore, they have key roles in the establishment, diversification, productivity, and sustainability of different natural ecosystems. However, some of these communities are pathogenic for the plant itself or dangerous for the consumers, due to the production of mycotoxins. In other words, plant mycobiome represents two faces of a coin. This book aimed to explore contributions of the plant mycobiome in plant-environment interactions from different perspectives. Chapters of this book address numerous themes covering the recent advances in roles, diversity, and dynamics of these fungi as biocontrol agents, biofertilizers, growth promoters, and their secondary metabolites in the area of crop improvement for sustainability and biotechnology, as well as the plant pathogenic and toxigenic fungi. This book will be useful to postgraduate students, botanists, mycologists, ecologists, plant pathologists, and physiologists.
New And Future Developments In Microbial Biotechnology And Bioengineering
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Author : Joginder Singh Panwar
language : en
Publisher: Elsevier
Release Date : 2020-06-25
New And Future Developments In Microbial Biotechnology And Bioengineering written by Joginder Singh Panwar and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-25 with Technology & Engineering categories.
New and Future Developments in Microbial Biotechnology and Bioengineering: Recent Advances in Application of Fungi and Fungal Metabolites: Biotechnological Interventions and Futuristic Approaches is an invaluable resource for researchers planning to work in applied biotechnological interventions and futuristic approaches to fungi and fungal metabolite utilization. Special emphasis is placed on new research relating to fungal-based recombinant DNA technology and genomics analysis which place yeasts and filamentous fungi at the forefront of various contemporary commercial applications. Written in an easy-to-follow language by active researchers, the book presents cutting-edge fungal biotechnological applications in a manner that is accessible to all. - Introduces recent biotechnological interventions and futuristic approaches to fungi and their metabolites - Elaborates on perspectives and diverse applications of harnessing the potential of fungi and fungal metabolites in biotechnology - Describes traditional uses and modern practices of accessing the potential of fungi and their metabolites in solving future needs
Index Medicus
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Author :
language : en
Publisher:
Release Date : 2003
Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medicine categories.
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Systems Biotechnology Of Recombinant Protein Production In Aspergillus Niger
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Author : Habib Driouch
language : en
Publisher: Cuvillier Verlag
Release Date : 2011-07-11
Systems Biotechnology Of Recombinant Protein Production In Aspergillus Niger written by Habib Driouch and has been published by Cuvillier Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-11 with Science categories.
The filamentous fungus Aspergillus niger is an important efficient microbial cell factory for industrial production of enzymes as well as organic acids or antibiotics. In submerged cultivation, A. niger exhibits a rather complex morphology which typically has a strong influence on production performance. Although difficult to control so far, the morphological shape is obviously linked to key production characteristics of fungal cell factories. In this regard, comprehensive approaches, combining systems wide analysis and optimization at the cellular level with process driven engineering of the bioreactor environment, seem most useful in order to achieve superior production processes. This was applied to recombinant proteins production in A. niger. The optimization included the use of talc or alumina micro particles, added to the culture, which allowed to the precise controlling the morphological shape of A. niger and increase enzyme production in different recombinant strains. Additionally, the targeted engineering of the morphology of A. niger into high-producing bio-pellet forms of various sizes by the addition of titanate micro particles was demonstrated. This strategy was combined with model-based medium design and development of efficient fed-batch strategies to optimize the production of the high-value enzyme fructofuranosidase, an important biocatalyst for neo-sugar in food or pharmaceutical industry, in the recombinant strain A. niger SKAn1015. As a result, the achieved enzyme titre could be increased to 2,800 U/mL, more than tenfold as compared to previously described processes. The enzyme, obtained by this micro particle enhanced process, could be applied as biocatalyst with minimal pre-treatment for the biosynthesis of 450 g/L of neo-sugar of the inulin type, such as 1 kestose and 1 nystose, which all display pre-biotics with substantial commercial interest. These studies were complementary by systems biotechnology analysis of A. niger as it is expected that this may lead to increased understanding of the context of cellular metabolism, regulation and thus further improvement of strains and processes. Fluxome analyses by 13C isotope studies and in silico design are applied to quantify the underlying carbon core metabolism of A. niger under different conditions. This yielding valuable insights towards the tailor made design of A. niger as a cell factory for recombinant protein production.
Fungal Cellulolytic Enzymes
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Author : Xu Fang
language : en
Publisher: Springer
Release Date : 2018-08-31
Fungal Cellulolytic Enzymes written by Xu Fang and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-31 with Medical categories.
The book provides an overview of the current knowledge on cellulolytic enzymes and their applications. It summarizes the mechanisms of synthesis and hydrolysis of cellulolytic enzymes, industrial fungal strains, genetic engineering of fungal strains and application of cellulolytic enzymes. This book will be a useful reference for researchers and bioengineering experts engaged in lignocelluloses biodegradation, biomass utilization, enzyme production and fungal molecular biology.
Genetic Improvement Of Enzyme Production In Industrially Important Fungal Strains
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Author : Helena Nevalainen
language : en
Publisher:
Release Date : 1985
Genetic Improvement Of Enzyme Production In Industrially Important Fungal Strains written by Helena Nevalainen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Enzymes categories.
Enzymes And Products From Bacteria Fungi And Plant Cells
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Author :
language : en
Publisher: Springer
Release Date : 2013-11-20
Enzymes And Products From Bacteria Fungi And Plant Cells written by and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-20 with Science categories.
Ch. P. Kubicek, Vienna: "The Cellulase Proteins of Trichoderma reesei: Structure, Multiplicity, Mode of Action and Regulation of Formation" Dr.A. Singh, Dr. P.K.R. Kumar, Dr. K. Sch}gerl, Hannover: "Bioconversion of Cellulosic Materials to Ethanol by Filamentous Fungi" Dr. T. Coolbear, Palmerston North, New Zealand, Dr. R.M. Da- niel, Dr. H.W. Morgan, Hamilton,New Zealand: "The Enzymes from Extreme Thermophiles: Bacterial Sources, Thermostabilities and Industrial Relevance" Dr. N. Weber, M}nster, Dr. D. C. Taylor, Dr. E. W. Under- hill, Saskatoon (Canada): "Biosynthesis of Storage Lipids in Plant Cell and Embryo Cultures"
Recombinant Protein Expression In Microbial Systems
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Author : Eduardo A. Ceccarelli
language : en
Publisher: Frontiers E-books
Release Date : 2014-10-02
Recombinant Protein Expression In Microbial Systems written by Eduardo A. Ceccarelli and has been published by Frontiers E-books this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-02 with Biotechnology categories.
With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.
Optimization Of Recombinant Protein Production By A Fungal Host
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Author : Reza Gheshlaghi
language : en
Publisher:
Release Date : 2007
Optimization Of Recombinant Protein Production By A Fungal Host written by Reza Gheshlaghi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.