Analysis And Detection By Capillary Electrophoresis
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Analysis And Detection By Capillary Electrophoresis
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Author : Maria Luisa Marina
language : en
Publisher: Elsevier
Release Date : 2005-09-06
Analysis And Detection By Capillary Electrophoresis written by Maria Luisa Marina and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-09-06 with Science categories.
Capillary Electrophoresis (CE) is a powerful analytical technique used to separate compounds and is increasingly being used in routine analytical laboratories. Analysis and Detection by Capillary Electrophoresis presents developments enabling the enhancement of the detection sensitivity in CE, including the different strategies used to achieve sensitivity requirements. It describes techniques allowing sample preconcentration and sensitive continuous detection systems and looks at recent developments such as chiral analysis in CE and electrochemical detection in microchips. UV-Vis absorbance detection, as the most widely used detection system in CE, is also presented. Analysis and Detection by Capillary Electrophoresis delves into the practical approaches used in the field and will greatly benefit analytical chemists, as well as students, teachers, technical analysts, scientists and researchers involved in capillary electrophoresis.* An overview of practical approaches employed in capillary electrophoresis, including the fundamentals, instrumentation, and applications of continuous detections systems employed in capillary electrophoresis* Covers aspects of capillary electrophoresis that have never previously been covered * Written by leading experts in the field
Capillary Electrophoresis
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Author : Patrick Camilleri
language : en
Publisher: CRC Press
Release Date : 1997-11-25
Capillary Electrophoresis written by Patrick Camilleri and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-11-25 with Medical categories.
The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.
Analysis Of Carbohydrates By Capillary Electrophoresis
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Author : Aran Paulus
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Analysis Of Carbohydrates By Capillary Electrophoresis written by Aran Paulus and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Technology & Engineering categories.
Carbohydrates are ubiquitous. They come as monomers, oligomers and polymers with a startling variety of chemical, physical and material properties. They affect almost every as pect of human live. For example they are an essential part of our nutrition. Cellulose, a car bohydrate polymer, acts as a central material in civilization: as building material for housing and as paper for written communication. Scientifically, the interest in carbohydrates ranges from structural elucidation of simple carbohydrates to fundamental biochemical processes such as photosynthesis and glycolysis. Always, a deeper understanding of a particular aspect of carbohydrate chemistry was accompanied by new methods of measuring and characteri zing this class of molecules. During the last decades a better understanding of the roles of carbohydrates in biochemi cal pathways developed. It turned out, that carbohydrates attached to proteins in glycoprote ins are involved in a number of molecular recognition and targeting processes. This lead to a new research field: Glycobiology. However, unlike for proteins or nucleic acids, there is at this time no consistent theory how chemical composition and structure controls function and inherent information. In order to develop the database, on which new theories and scientific hypothesis are founded, analytical methods to separate compounds with an enormous struc tural diversity and only minor differences in chemical or physical properties has to be deve loped, preferably using only minute quantities.
Capillary Electrophoresis
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Author : Philippe Schmitt-Kopplin
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-25
Capillary Electrophoresis written by Philippe Schmitt-Kopplin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-25 with Science categories.
This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.
Analysis Of Pharmaceuticals By Capillary Electrophoresis
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Author : Kevin D. Altria
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Analysis Of Pharmaceuticals By Capillary Electrophoresis written by Kevin D. Altria and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Technology & Engineering categories.
During the 1980's the analysis of pharmaceuticals was dominated by the use of High Perfor mance Liquid Chromatography (HPLC). Other separative techniques such as Gas Chromato graphy and Thin Layer Chromatography offered alternatives but their quantitative capabili ties and/or solute range could not approach that of HPLC. The majority of pharmaceuticals are ionic and it would be reasonable to assume that electrophoresis may be useful in the analysis of pharmaceuticals. However, the electrophore tic instruments available in the 1980's were labour intensive and employed post-separation detection procedures. During the late 1980's and early 1990's extensive research was con ducted into the possibilities of conducting electrophoretic separations in capillaries. This approach allowed on-line detection and could be performed on fully automated equipment. This research led to the advent of modern day capillary electrophoresis (CE) instruments which offer similar performance and automation levels to that of HPLC. Research was also focused on developing applications for CE and particular attention was paid to applications within the pharmaceutical analysis area. These applications proved that CE could be applied to a wide range of drug types including water insoluble and neutral compounds. The ability to achieve efficient chiral separations of drugs also increased the popularity of the technique. CE with indirect UV detection has become established as a simple and effective alternative to ion-exchange chromatography for the determination of small inorganic or organic ions.
Analysis Of Peptides And Proteins By Electrophoretic Techniques
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Author : Angela R. Piergiovanni
language : en
Publisher: MDPI
Release Date : 2019-07-23
Analysis Of Peptides And Proteins By Electrophoretic Techniques written by Angela R. Piergiovanni and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-23 with Science categories.
The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Analytical Separation Science 5 Volume Set
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Author : Jared Anderson
language : en
Publisher: John Wiley & Sons
Release Date : 2016-02-29
Analytical Separation Science 5 Volume Set written by Jared Anderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-02-29 with Science categories.
Endlich ein Forschungsleitfaden für Wissenschaftler des Fachgebiets, die neue Methoden entwickeln oder einsetzen. Dieses Handbuch umfasst fünf thematische Bände und bietet damit einen umfassenden Überblick über das Fachgebiet. Erläutert werden Grundlagen, die Methodenentwicklung und hochkarätige Anwendungen für alle wichtigen Analyseverfahren, darunter chromatische Verfahren, Techniken in den Bereichen Elektromigration und Membranen. Dieses Referenzwerk umfasst ein breites Spektrum und legt den Schwerpunkt auf Entwicklungen für die Zukunft. Damit ist es ein Muss für Forscher und eine wertvolle Wissensquelle für Studenten im Hauptstudium und Studienabsolventen.
Handbook Of Capillary Electrophoresis Second Edition
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Author : James P. Landers
language : en
Publisher: CRC Press
Release Date : 1996-12-23
Handbook Of Capillary Electrophoresis Second Edition written by James P. Landers and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-12-23 with Medical categories.
Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
Capillary Electrophoresis In Analytical Biotechnology
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Author : Pier Giorgio Righetti
language : en
Publisher: CRC Press
Release Date : 1995-12-18
Capillary Electrophoresis In Analytical Biotechnology written by Pier Giorgio Righetti and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-12-18 with Medical categories.
This new book on capillary electrophoresis (CE) is unique in its focus on biotechnology. It is devoted to proteins, peptides, and techniques especially useful in the area of recombinant DNA products. Emphasis is also placed on glycoproteins. Because of the growing role of the glycosylation process in CE, a comprehensive chapter on the subject acts as a book within a book. Although this well-known researcher in biotechnology presents a number of chapters extensively discussing theories, important practical aspects in the routine use of capillary electrophoresis are also covered.
Analysis Of Nucleic Acids By Capillary Electrophoresis
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Author : Christoph Heller
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Analysis Of Nucleic Acids By Capillary Electrophoresis written by Christoph Heller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Technology & Engineering categories.
During the last decade, capillary electrophoresis has been developed into a very powerful analytical technique, which has many advantages over conventional slab gel techniques. The improvement is comparable to the one occuring earlier in the field of chromatography, with the introduction of the high performance technology. How important this technique has be come, is reflected by the shear volume of papers published each month; as well as a dozen of books already published on this subject. One of the most important conferences in the field, the "International Symposium on High Performance Capillary Electrophoresis" attracts now nearly thousand people every year. As capillary electrophoresis can be applied to many different analytical problems, a spe cialization is unavoidable. This evolution is also reflected in the development of instrumen tation: whereas the first devices were designed for all possible applications, new instruments are now built, that are specialized for one particular task, e.g. DNA analysis. I very much welcome the decision of the series editor and the publisher, to edit a series ofspecialized books, covering all aspects of capillary electrophoresis. Having worked on the electrophoretic separation of DNA for many years, I am convinced that there are so many different aspects on this issue that they deserve a whole book on their own. Therefore, I was happy to agree when being asked to edit this book.