Angiogenesis Protocols
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Angiogenesis Protocols
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Author : J. Clifford Murray
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Angiogenesis Protocols written by J. Clifford Murray and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
In the last few years, we have been deluged with information on ang- genesis. Scientists and the public at large are exposed daily to this “new” science, not just in specialist journals and texts, but in the tabloid press, where popular articles refer to angiogenic therapies as magic bullets and miracle cures for cancer, arthritis, retinopathies, heart disease, and circulatory problems. Is there no ill this approach will not cure? The fact that so much time, effort, and resource have been and continue to be dedicated to this new science is clear testament to its importance. Yet many fundamental aspects of angiogenesis remain poorly und- stood, in particular cues that activate the process. This fact has to some extent been masked behind a surfeit of fine detail; we can’t see the wood for the trees. Most studies of angiogenesis identify single links in a long chain of events. Furthermore, each study is itself hampered by the limitations of the biological end-point chosen. For instance, though endothelial proliferation may well be necessary for angiogenesis, it is not sufficient. Therefore, measuring endothelial proliferation in response to a novel growth factor, and on the basis of this obs- vation, stating that the factor is “angiogenic,” is unsound logic. It is important that researchers in this field, and perhaps more importantly those experimenting at its periphery, recognize the limitations of their chosen biological end-points.
Angiogenesis
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Author : Andrew V. Benest
language : en
Publisher:
Release Date : 2022
Angiogenesis written by Andrew V. Benest and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Blood-vessels--Growth categories.
This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Angiogenesis: Methods and Protocols provides a meaningful compendium of practical approaches to angiogenesis and endothelial cell biology research.
Tumor Angiogenesis Assays
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Author : Domenico Ribatti
language : en
Publisher: Springer Nature
Release Date : 2022-09-26
Tumor Angiogenesis Assays written by Domenico Ribatti and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-09-26 with Medical categories.
This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
Cerebral Angiogenesis
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Author : Richard Milner
language : en
Publisher: Humana
Release Date : 2016-08-27
Cerebral Angiogenesis written by Richard Milner and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-27 with Medical categories.
Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process. Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain.
Angiogenesis Protocols
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Author : Stewart Martin
language : en
Publisher: Humana Press
Release Date : 2014-10-30
Angiogenesis Protocols written by Stewart Martin and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-30 with Medical categories.
As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular BiologyTM series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls. Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.
Angiogenesis In Vivo Systems
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Author : David A. Cheresh
language : en
Publisher: Academic Press
Release Date : 2009-02-12
Angiogenesis In Vivo Systems written by David A. Cheresh and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-02-12 with Science categories.
Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over angiogenesis. Diseases that are angiogenesis-dependent result when blood vessels either grow excessively or insufficiently. Understanding how angiogenesis "works" and how to control it, will have massive implications on the management, treatments, and ultimately the prevention of many common (and not so common) diseases. Angiogenesis cuts across virtually every discipline. The Angiogenesis Foundation identified angiogenesis as a "common denominator" in our most serious diseases. Excessive angiogenesis occurs in diseases such as cancer, diabetic blindness, age-related macular degeneration, rheumatoid arthritis, psoriasis, and many other conditions. Insufficient angiogenesis occurs in diseases such as coronary artery disease, stroke, and delayed wound healing. * Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition. * Provides the "builder's manual" for essential techniques. This is a one-stop shop that eliminates needless searching among untested techniques. * Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics
Angiogenesis In Vitro Systems
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Author : David A. Cheresh
language : en
Publisher: Academic Press
Release Date : 2011-09-02
Angiogenesis In Vitro Systems written by David A. Cheresh and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-02 with Science categories.
Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently. * Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition * Provides the "builder's manual" for essential techniques--a one-stop shop that eliminates needless searching among untested techniques * Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics
Lymphangiogenesis
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Author : Guillermo Oliver
language : en
Publisher: Humana
Release Date : 2019-10-06
Lymphangiogenesis written by Guillermo Oliver and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-06 with Science categories.
This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field.
Angiogenesis In Vivo Systems
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Author :
language : en
Publisher: Academic Press
Release Date : 2009-02-12
Angiogenesis In Vivo Systems written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-02-12 with Science categories.
Understanding how angiogenesis "works" and how to control it will have massive implications on the management, treatments, and ultimately the prevention of many common (and not so common) diseases. Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over angiogenesis. Diseases that are angiogenesis-dependent result when blood vessels either grow excessively or insufficiently. * Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition * Provides the "builder's manual" for essential techniques--a one-stop shop that eliminates needless searching among untested techniques * Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics
Wound Healing
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Author : Luisa A. DiPietro
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Wound Healing written by Luisa A. DiPietro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Medical categories.
During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to these many disciplines has generated great interest in models and methods for the study of wound healing. The goal of Wound Healing: Methods and Protocols is to provide scientists from many dis- plines with a compendium of classic and contemporary protocols from r- ognized experts in the field of wound healing. We hope this volume will be useful not only to those working within the field itself, but also to scientists from other disciplines who wish to adapt wound healing models to their own experimental needs. The process of wound healing encompasses many different biologic p- cesses, including epithelial growth and differentiation, fibrous tissue prod- tion and function, angiogenesis, and inflammation.