Antibody Methods And Protocols

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Antibody Methods And Protocols

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Author : Gabriele Proetzel
language : en
Publisher: Humana Press
Release Date : 2016-05-01

Antibody Methods And Protocols written by Gabriele Proetzel and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Medical categories.

This Methods in Molecular Biology volume covers in vitro and in vivo generation of antibodies, as well as techniques for screening, analysis and modification of antibodies and antibody fragments. Offers materials lists, protocols and troubleshooting tips."

Antibody Engineering

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Author : Damien Nevoltris
language : en
Publisher: Humana Press
Release Date : 2018-09-24

Antibody Engineering written by Damien Nevoltris and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-09-24 with Medical categories.

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Monoclonal Antibodies

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Author : Vincent Ossipow
language : en
Publisher: Humana Press
Release Date : 2014-02-28

Monoclonal Antibodies written by Vincent Ossipow and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-28 with categories.

Human Monoclonal Antibodies

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Author : Michael Steinitz
language : en
Publisher: Humana
Release Date : 2018-12-12

Human Monoclonal Antibodies written by Michael Steinitz and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-12-12 with Medical categories.

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

Single Domain Antibodies

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Author : Dirk Saerens
language : en
Publisher: Humana Press
Release Date : 2012-08-10

Single Domain Antibodies written by Dirk Saerens and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-10 with Medical categories.

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Peptide Antibodies

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Author : Gunnar Houen
language : en
Publisher: Springer Nature
Release Date :

Peptide Antibodies written by Gunnar Houen and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.

Single Domain Antibodies

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Author : Greg Hussack
language : en
Publisher: Springer Nature
Release Date : 2022-02-14

Single Domain Antibodies written by Greg Hussack and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-14 with Science categories.

This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.

Elisa

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Author : Robert S. Matson
language : en
Publisher: Springer Nature
Release Date : 2023-02-16

Elisa written by Robert S. Matson and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-16 with Medical categories.

This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.

Therapeutic Antibodies

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Author : Antony S. Dimitrov
language : en
Publisher: Humana Press
Release Date : 2014-11-25

Therapeutic Antibodies written by Antony S. Dimitrov and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-25 with Medical categories.

Over 2000 years ago in China, antibodies elicited by early forms of vaccination likely played a major role in the protection of the population from infectious agents. Vac- nation has been further developed in Europe and described by Edward Jenner in the late-eighteenth century, then successfully implemented worldwide. The idea to use theactiveingredientinthebloodofvaccinated(orimmunized)animalsorhumansfor the treatment of diseases came a century later. It was made possible by a series of discoveries,suchastherealizationthattheserumfromanimalsimmunizedwithtoxins, for example, diphtheria toxin or viruses, is an effective therapeutic against the disease causedbythesameagentinhumans. Inthe1880s,vonBehringdevelopedanantitoxin (anti-body) that did not kill the bacteria but neutralized the bacterial toxin. The first Nobel Prize in Medicine (1901) was given to him for the discovery of the serum therapy. Acenturylater,22monoclonalantibodies(mAbs)areapprovedbytheUnited States Food and Drug Administration (FDA) for clinical use, and hundreds are in clinicaltrialsforthetreatmentofvariousdiseasesincludingcancers,immunedisorders, and infections. The revenues from the top-five therapeutic antibodies reached $11. 7 billion in 2006, and major pharmaceutical companies raced to acquire antibody biotech companies with a recent example of MedImmune, Inc. , which was acquired for $15. 6 billion by AstraZeneca in 2007. This explosion of research and development in the field of therapeutic antibodies prompted the publication of the MiMB volume Therapeutic Antibodies: Methods and Protocols. The book’s major goal is to present a set of protocols useful for researchers discoveringanddevelopingtherapeuticantibodies. Currentadvancesandfuturetrends in the antibody therapeutics are analyzed in the lead-in review article.

Antibody Engineering

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Author : Patrick Chames
language : en
Publisher: Humana Press
Release Date : 2016-08-23

Antibody Engineering written by Patrick Chames and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.

More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an average rate of 50 per year and that is predicted to continue well into the future. Notwithstanding the many achievements already made in the field, there is still a lot of room for improvements for these molecules in terms of activity, and a plethora of approaches have been attempted to optimize these molecules. Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Antibody Engineering: Methods and Protocols, Second Edition serves as an invaluable resource for both experts and those new to the field, and most of all as a source of inspiration for the creation of the antibodies of tomorrow.