Automated Aberration Correction For Sted Microscopy
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Automated Aberration Correction For Sted Microscopy
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Author : Debadrita Ghosh
language : en
Publisher:
Release Date : 2023
Automated Aberration Correction For Sted Microscopy written by Debadrita Ghosh and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
Stimulated emission depletion (STED) microscopy is a powerful super resolution fluorescence microscopy technique that surpasses the diffraction limit. However, the performance of a STED microscope can be adversely influenced by aberrations. In thick biological specimens, aberrations are primarily induced by wavefront distortion caused by variations in the refractive index within the specimen. These aberrations have a substantial impact on the quality of the acquired images and impose limitations on the achievable resolution. This problem can be resolved by using adaptive optics (AO) in a fe...
Aberration Correction In Sted Microscopy
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Author : Joris van Dort
language : en
Publisher:
Release Date : 2018
Aberration Correction In Sted Microscopy written by Joris van Dort and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
In the last decade, superresolution microscopy techniques became a valuable tool to study sub-cellular structures with a resolution below the diffraction limit. The use of superresolution for brain research requires to super-resolve structures deep within the tissue of the living mouse brain. The only superresolution technique applied in the living mouse brain so far is stimulated emission depletion (STED) microscopy. However, the penetration depth of STED microscopy in the cortex of a living mouse has been limited to a depth of 40æm. In this thesis, I studied the use of adaptive optics wit ...
Aberration Corrected Imaging In Transmission Electron Microscopy
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Author : Rolf Erni
language : en
Publisher: World Scientific
Release Date : 2010
Aberration Corrected Imaging In Transmission Electron Microscopy written by Rolf Erni and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Science categories.
This book provides a concise introduction to practical aspects of atomic-resolution imaging in aberration-corrected electron microscopy. As such, it addresses recent advances in electron optical instrumentation used for ultra-high resolution imaging in materials and nano-science. It covers two of the most popular atomic resolution imaging techniques' namely high-resolution transmission electron microscopy and scanning transmission electron microscopy. The book bridges the gap between application-oriented textbooks in conventional electron microscopy and books in physics covering dedicated topics in charged-particle optics and aberration correction. The book is structured in three parts which can be read separately. While in the first part the fundamentals of the imaging techniques and their limits in conventional electron microscopes are explained, the second part provides readers with the basic principles of electron optics and the characteristics of electron lenses. The third part, focusing on aberrations, describes the functionality of aberration correctors and provides readers with practical guidelines for the daily work with aberration-corrected electron microscopes. The book represents a detailed and easy readable guide to aberration-corrected electron microscopy.
Advances In Nano Scale Systems With Optics Nano Chemical Nanomaterial And Nano Biomedicine
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Author : Honghui He
language : en
Publisher: Frontiers Media SA
Release Date : 2022-09-22
Advances In Nano Scale Systems With Optics Nano Chemical Nanomaterial And Nano Biomedicine written by Honghui He and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-09-22 with Science categories.
Aberration Corrected Imaging In Transmission Electron Microscopy
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Author : Rolf Erni
language : en
Publisher:
Release Date : 2015
Aberration Corrected Imaging In Transmission Electron Microscopy written by Rolf Erni and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Electronic books categories.
Three Dimensional And Multidimensional Microscopy
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Author :
language : en
Publisher:
Release Date : 2006
Three Dimensional And Multidimensional Microscopy written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Confocal microscopy categories.
Imaging With Aberration Corrected Low Energy Electron Microscopy
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Author : Sebastian Markus Schramm
language : en
Publisher:
Release Date : 2013
Imaging With Aberration Corrected Low Energy Electron Microscopy written by Sebastian Markus Schramm and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Power Focus Robust Aberration Correction In Electron Microscopy
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Author : Rangoli Saxena
language : en
Publisher:
Release Date : 2024
Power Focus Robust Aberration Correction In Electron Microscopy written by Rangoli Saxena and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024 with categories.
Far Field Optical Nanoscopy
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Author : Philip Tinnefeld
language : en
Publisher: Springer
Release Date : 2015-02-07
Far Field Optical Nanoscopy written by Philip Tinnefeld and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-02-07 with Science categories.
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Three Dimensional Aberration Corrected Scanning Transmission Electron Microscopy For Biology
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Author :
language : en
Publisher:
Release Date : 2007
Three Dimensional Aberration Corrected Scanning Transmission Electron Microscopy For Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Recent instrumental developments have enabled greatly improved resolution of scanning transmission electron microscopes (STEM) through aberration correction. An additional and previously unanticipated advantage of aberration correction is the greatly improved depth sensitivity that has led to the reconstruction of a three-dimensional (3D) image from a focal series. In this chapter the potential of aberration-corrected 3D STEM to provide major improvements in the imaging capabilities for biological samples will be discussed. This chapter contains a brief overview ofthe various high-resolution 3D imaging techniques, a historical perspective of the development of STEM, first estimates of the dose-limited axial and lateral resolution on biological samples and initial experiments on stained thin sections.