Basic Cell Culture
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Basic Cell Culture Protocols
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Author : Jeffrey W. Pollard
language : en
Publisher: Springer Science & Business Media
Release Date : 1997
Basic Cell Culture Protocols written by Jeffrey W. Pollard and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Animals categories.
Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
Basic Cell Culture
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Author : J. M. Davis
language : en
Publisher:
Release Date : 2002
Basic Cell Culture written by J. M. Davis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Basic Cell Culture Protocols
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Author : Cheryl D. Helgason
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Basic Cell Culture Protocols written by Cheryl D. Helgason and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
Freshney S Culture Of Animal Cells
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Author : R. Ian Freshney
language : en
Publisher: John Wiley & Sons
Release Date : 2021-02-17
Freshney S Culture Of Animal Cells written by R. Ian Freshney and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-02-17 with Science categories.
FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.
Basic Cell Culture 2 E
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Author : John M. Davis
language : en
Publisher:
Release Date : 2005-03-07
Basic Cell Culture 2 E written by John M. Davis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-03-07 with Cell culture categories.
General Techniques Of Cell Culture
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Author : Maureen A. Harrison
language : en
Publisher: Cambridge University Press
Release Date : 1997-10-13
General Techniques Of Cell Culture written by Maureen A. Harrison and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-10-13 with Science categories.
Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.
Culture Of Animal Cells
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Author : R. Ian Freshney
language : en
Publisher:
Release Date : 1987
Culture Of Animal Cells written by R. Ian Freshney and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.
Cultured!cell - Biohazards - Sterilization - Cell!lines - Cloning - Specific!cell!types - Cell!separation - Transformed!phenotype - Cytotoxicity - Culture!of!specific!cell!types - Culture!of!tumor!tissue - Three!dimensional!culture!systems.
Basic Cell Culture
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Author : John M. Davis
language : en
Publisher: Oxford University Press, USA
Release Date : 1994
Basic Cell Culture written by John M. Davis and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
In the last 20 years cell culture has developed enormously from being used only in specialized areas of research to being the cornerstone of probably the world's fastest-growing industry, biotechnology. The primary aim of this book is to guide the newcomer progressively through all those areas which nowadays are fundamental to the performance of cell culture. The book will also prove useful to the experienced worker. Topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, culture technique, the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination, and good laboratory practice in the cell culture laboratory.
Plant Cell Culture
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Author : Michael R. Davey
language : en
Publisher: John Wiley & Sons
Release Date : 2010-05-20
Plant Cell Culture written by Michael R. Davey and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05-20 with Science categories.
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
Culture Of Animal Cells
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Author : R. Ian Freshney
language : en
Publisher: John Wiley & Sons
Release Date : 2015-12-23
Culture Of Animal Cells written by R. Ian Freshney and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-23 with Science categories.
Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.