Basic Methods In Molecular Biology
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Basic Methods In Molecular Biology
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Author : Leonard Davis
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Basic Methods In Molecular Biology written by Leonard Davis and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.
Basic Methods In Molecular Biology
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Author : Leonard G. Davis
language : en
Publisher:
Release Date : 1992
Basic Methods In Molecular Biology written by Leonard G. Davis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Molecular biology categories.
The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another.
Basic Techniques In Molecular Biology
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Author : Stefan Surzycki
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Basic Techniques In Molecular Biology written by Stefan Surzycki and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Molecular Biology Techniques
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Author : Walt Ream
language : en
Publisher: Academic Press
Release Date : 1998-11-17
Molecular Biology Techniques written by Walt Ream and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-17 with Science categories.
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter
Molecular Biology Techniques
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Author : Heather Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Overrun Edition
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Author : Appleton & Lange
language : en
Publisher:
Release Date : 1994-03-01
Overrun Edition written by Appleton & Lange and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-03-01 with Molecular biology categories.
Basic Techniques In Biochemistry Microbiology And Molecular Biology
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Author : Aakanchha Jain
language : en
Publisher: Humana
Release Date : 2021-03-14
Basic Techniques In Biochemistry Microbiology And Molecular Biology written by Aakanchha Jain and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-03-14 with Medical categories.
This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.
Diagnostic Molecular Biology
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Author : Chang-Hui Shen
language : en
Publisher: Elsevier
Release Date : 2023-06-29
Diagnostic Molecular Biology written by Chang-Hui Shen and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-06-29 with Science categories.
Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)
Guide To Yeast Genetics And Molecular Biology
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Author : Christine Guthrie
language : en
Publisher:
Release Date : 1991
Guide To Yeast Genetics And Molecular Biology written by Christine Guthrie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Molecular biology categories.
Basic Methods In Antibody Production And Characterization
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Author : Gary C. Howard
language : en
Publisher: CRC Press
Release Date : 2000-09-21
Basic Methods In Antibody Production And Characterization written by Gary C. Howard and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-09-21 with Medical categories.
Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described