Basics Of Crispr Cas Mediated Plant Genome Editing
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Basics Of Crispr Cas Mediated Plant Genome Editing
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Author : Anshu Alok
language : en
Publisher: CRC Press
Release Date : 2024-08-23
Basics Of Crispr Cas Mediated Plant Genome Editing written by Anshu Alok and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-08-23 with Science categories.
CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture free editing which makes it easier for crop improvement. With such technologies, application of genome editing has opened new paths for crop improvements and sustainable agriculture. The current book will be useful for beginners to understand these tools and how to use them in a better and more efficient way.
Crispr Cas Mediated Genome Editing In Plants
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Author : Deepu Pandita
language : en
Publisher: CRC Press
Release Date : 2023-06-30
Crispr Cas Mediated Genome Editing In Plants written by Deepu Pandita and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-06-30 with Science categories.
With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops
Genome Editing In Plants
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Author : Om Prakash Gupta
language : en
Publisher: CRC Press
Release Date : 2021-07-25
Genome Editing In Plants written by Om Prakash Gupta and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-25 with Science categories.
Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops
Gene Editing In Plants
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Author : Ashwani Kumar
language : en
Publisher: Springer Nature
Release Date :
Gene Editing In Plants written by Ashwani Kumar and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Plant Genome Editing With Crispr Systems
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Author : Yiping Qi
language : en
Publisher: Springer Nature
Release Date : 2019-01-04
Plant Genome Editing With Crispr Systems written by Yiping Qi and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-04 with Science categories.
This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Genome Editing
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Author : Shabir Hussain Wani
language : en
Publisher: Springer Nature
Release Date : 2022-11-08
Genome Editing written by Shabir Hussain Wani and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-08 with Science categories.
Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Crispr Cas Genome Editing
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Author : Anjanabha Bhattacharya
language : en
Publisher: Springer Nature
Release Date : 2020-12-11
Crispr Cas Genome Editing written by Anjanabha Bhattacharya and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-11 with Science categories.
This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.
Crispr Crops
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Author : Aftab Ahmad
language : en
Publisher: Springer Nature
Release Date : 2021-01-08
Crispr Crops written by Aftab Ahmad and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-08 with Medical categories.
This book compiles the latest applications of the cutting-edge gene editing tool CRISPR/Cas in the area of crop improvement. It begins with an introduction to the technique and its application in crop plants. Next, it gives an updated overview of available delivery methods, design tools and resources in CRISPR/Cas. The book subsequently reviews the applications of CRISPR/Cas in connection with e.g. insect stress, disease stress, abiotic stress, nutritional and yield improvement in crop plants, etc. It also discusses the various regulatory, ethical and social aspects of the technique that must be kept in mind when designing experiments. In closing, the book summarizes the status quo and outlines future prospects for the tool in crop improvement and food security. Given its scope, the book will especially benefit students and researchers in food science, biotechnology, agriculture and the plant sciences.
A Roadmap For Plant Genome Editing
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Author : Agnès Ricroch
language : en
Publisher: Springer Nature
Release Date : 2024-01-15
A Roadmap For Plant Genome Editing written by Agnès Ricroch and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-01-15 with Science categories.
This open access book is an update of genome editing techniques applied to a range of plants. We discuss the latest techniques and applications to cereals, roots and tubers, oilseed crops, fruit and forest trees, vegetables, legumes and algae including resistance to biotic and abiotic stresses, improved quality, drug production, yield and adaptation to climate change. The regulations in different countries worldwide, the patentibility and the perception by society of the applications of new genomic techniques are examined. This book is written by a multidisciplinary and multisectoral collective of high-profile scientists and other experts belonging to the COST Action network PlantEd, which is mainly European but with contributions from American, Australian, Canadian, Chinese, Indian, Iranian, Pakistani and Peruvian scientists. The book is aimed at a wide audience consisting of students, academics, private and public breeders, other actors in the food and bioeconomy value chains and policy and law makers. This is an open access book.
Plant Genome Editing Technologies
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Author : Jen-Tsung Chen
language : en
Publisher: Springer Nature
Release Date :
Plant Genome Editing Technologies written by Jen-Tsung Chen and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.