Biochemical Techniques In The Heart
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Biochemical Techniques In The Heart
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Author : John H. McNeill
language : en
Publisher: CRC Press
Release Date : 1996-12-12
Biochemical Techniques In The Heart written by John H. McNeill and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-12-12 with Medical categories.
Biochemical Techniques in the Heart fully describes classical biochemical measurements of parameters involved in contraction and relaxation in the heart. This fully detailed guide tells you how to make preparations of sarcoplasmic reticulum, sarcolemma, and cardiomyocytes and how to measure sodium-calcium and sodium-hydrogen exchange. Two chapters explain the measurement of the important enzyme sodium-potassium ATPase. This book examines the most widely used tools in experimental cardiology and provides you with the recipe-setting up the technique, procurement of equipment, sample data and calculations, problems and trouble shooting, adapting to other species, modifications, and applicability. Undoubtedly, this text will be a great asset to cardiovascular physiologists, pharmacologists, experimental cardiologists, and students of physiology and pharmacology.
Cell Culture Techniques In Heart And Vessel Research
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Author : H.M. Piper
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cell Culture Techniques In Heart And Vessel Research written by H.M. Piper and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.
New Aspects In The Treatment Of Failing Heart
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Author : Hisakazu Yasuda
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
New Aspects In The Treatment Of Failing Heart written by Hisakazu Yasuda and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Molecular biology and biochemical techniques, the avant-garde of medical research, are now also being used to investigate cardiovascular events. The fifty-one selected contributions in this volume based on the 14th International Society of Heart Research (ISHR) Meeting in 1992 document the discoveries being made at the frontiers of research with cardiac molecular and genetic biology and technology. The contents may be divided into five categories: 1) the process of ischemic myocardial damage in heart failure, 2) biochemical abnormalities in cardiomyopathy, 3) signal transduction in diseased myocardial cells, 4) electrophysiological abnormalities of the failing heart, and 5) recent concepts in drug therapy. The book builds a bridge for the flow of up-to-date information from active veteran researchers to young talented investigators who offer great promise for the future.
Heart Proteomics
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Author : Fernando Vivanco
language : en
Publisher: Humana
Release Date : 2016-08-23
Heart Proteomics written by Fernando Vivanco and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years. Proteomics is a rapidly expanding investigation platform in cardiovascular medicine and is becoming integrated and incorporated into cardiovascular research. The proteomics field continues to develop with major improvements in mass spectrometry instrumentation, methodology and data analysis. Heart Proteomics: Methods and Protocols complies a selection of techniques and methods that target the numerous processes implicated in the pathophysiology of heart. Chapters cover protocols and updated methods in the heart proteomic area with a particular focus on MS-based methods of protein and peptide quantification and the analysis of posttranslational modifications as well as descriptions of system biology approaches, which provide a better understanding of normal and pathological processes. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Heart Proteomics: Methods and Protocols is a representative selection of methods that will prove to be a useful resource for experienced proteomics practitioners and newcomers alike.
Biochemical Mechanisms In Heart Function
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Author : Ernst-Georg Krause
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Biochemical Mechanisms In Heart Function written by Ernst-Georg Krause and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This special issue of Molecular and Cellular Biochemistry good source of information in this regard. contains original research papers as well as invited reviews We wish to thank all of the contributors for their help and dedicated, on the occasion of the 40th anniversary of the in cooperation. We also wish to thank Mrs. Verona Kuhle for auguration of the Heart Research Group in Beriin-Buch that her secretarial help. We are grateful to Dr. Naranjan S. Dhalla, today forms a part there ofthe Max Delbriick Center for Mo Editor-in-Chief of Molecular and Cellular Biochemistry for his lecular Medicine, to Professor Albert Wollenberger, founder interest and encouragement, and for agreeing to publish this of the Heart Research Group and for 21 years its head. issue in honor of Albert Wollenberger. The papers in this issue are written by researchers work ing in the field of cardiovascular research who together with ERNST-GEORG KRAUSE and ROLAND VETTER Albert Wollenberger share the belief that an integrative ap Max Delbriick Center for Molecular Medicine plication of advances in molecular and cellular biology will Robert-Rossle-StraBe 10 lead to new concepts for treatment and prevention of cardio 13122 Beriin-Buch vascular diseases. We hope that this special will serve as a Gennany ALBERT WOLLENBERGER, Professor, Ph. D. (Harvard), Dr. Sc. Med. (Berlin) The dedication is accorded to Prof.
Biophysics Of The Failing Heart
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Author : R. John Solaro
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-08-13
Biophysics Of The Failing Heart written by R. John Solaro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-13 with Science categories.
Subjects in the monograph “Biophysics of the Failing Heart” include state of the art chapters considering major biophysical mechanisms for why hearts responding to acquired or inherited stressors enter into maladaptive processes eventually leading to an inability of the heart to respond efficiently to hemodynamic loads especially during exercise. The chapters describe biophysical techniques that have been applied to determine the triggers for the heart failure process as well as the mechanisms for sustaining the disorders. These techniques include measurements of active and passive mechanical properties and hemodynamics at levels of organization ranging from molecules to hearts beating in situ. Biophysical concepts and approaches are also applied to determination of signaling and signal transduction, energetics, ionic currents, transport processes, electro-chemical and chemo-mechanical coupling. By its emphasis on biophysical aspects of a prevalent clinical condition, the monograph is unique in its perspective and focus. The breadth of information in the chapters all in one place will be of value to clinicians and researchers at all levels. Modern research approaches and clinical understanding of heart failure demands integration of multiple aspects of the disorders. In most cases, combinations of clinician scientists and researchers author the chapters. A main benefit of the book is couched in its didactic approach together with its emphasis on how biophysical concepts and techniques aid in diagnosis and development of new therapies.
New Data On Biochemistry Of The Heart Muscle Tissue As Evidenced By A Cytological Method Applicable To Toxicologic And Pharmacologic Research Work
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Author : P. Gallo
language : en
Publisher:
Release Date : 1961-11-30
New Data On Biochemistry Of The Heart Muscle Tissue As Evidenced By A Cytological Method Applicable To Toxicologic And Pharmacologic Research Work written by P. Gallo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1961-11-30 with categories.
The Developing Heart
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Author : Marianne J. Legato
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Developing Heart written by Marianne J. Legato and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Heart disease, despite recent improvements, continues to be the single most im portant cause of death and disability in the United States. It is estimated that the direct cost of medical care for cardiovascular disease is $6 billion dollars per year. Data compiled by the National Center for Health Statistics reveal a dramatic de cline in deaths from cardiovascular disease in the United States (greater than 20% since 1968). This phenomenon has been the subject of in-depth study. It is clear that the decline is real and not a statistical artifact. The decrease in mortality has been noted in all sections of the country, though the onset and rate of decline varies in different regions of the country. Both primary prevention, through changes in risk factors, and basic and applied research leading to earlier recognition and im proved treatment have contributed to the decline. They do not fully explain the decline. Further research is needed to clarify this issue. Clinical cardiologists have been exposed to a veritable explosion of new knowl edge of mechanisms of cardiovascular disease, development of new improved non-invasive diagnostic techniques, and the pharmacodynamics of agents affect ing the cardiovascular system. This new knowledge results from contributions made by individuals from diverse disciplines including cellular and molecular bi ologists, geneticists, hematologists, cardiologists, and cardiovascular surgeons.
Atp And The Heart
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Author : Joanne S. Ingwall
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Atp And The Heart written by Joanne S. Ingwall and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
ATP plays a central role in the two leading causes of cardiac morbidity and mortality in the western world: ischemia and heart failure. We are in our infancy applying what is known about biology and chemistry of ATP toward developing effective therapies for these diseases. In this volume, the current understanding of the chemistry and biology of ATP specifically in the cardiomyocyte is presented. New insights into ATP have been gleaned using biophysical techniques allowing dynamic measurement of chemical events in the intact beating heart and using new animal models in which cardiac proteins are either over expressed, deleted or harbor specific mutations. This book provides a summary of the basic understanding and includes illustrations of why ATP and the Heart is important to both the clinician and scientist.
Microscopy Of The Heart
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Author : Lars Kaestner
language : en
Publisher: Springer
Release Date : 2018-12-07
Microscopy Of The Heart written by Lars Kaestner and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-12-07 with Science categories.
This book provides in depths information on different microscopy approaches and supplies the reader with methods how to untangle highly complex processes involved in physiological and pathophysiological cardiac signaling. Microscopy approaches have established themselves as the quasi gold standard that enables us to appreciate the underlying mechanisms of physiological and pathophysiological cardiac signaling. This book presents the most important microscopy techniques from the level of individual molecule e.g. Förster-Resonance Energy Transfer (FRET), up to cellular and tissue imaging, e.g. electron microscopy (TEM) or light sheet microscopy. The book is intended for graduate students and postdocs in cardiovascular research, imaging and cell biology, pre-clinical and clinical researchers in cardiovascular sciences as well as decision makers of the pharmaceutical industry.