Cell Culture Techniques In Heart And Vessel Research
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Cell Culture Techniques In Heart And Vessel Research
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Author : H.M. Piper
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cell Culture Techniques In Heart And Vessel Research written by H.M. Piper and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.
Practical Methods In Cardiovascular Research
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Author : Stefan Dhein
language : en
Publisher: Springer
Release Date : 2006-02-28
Practical Methods In Cardiovascular Research written by Stefan Dhein and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-28 with Medical categories.
Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.
Endothelial Cell Culture
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Author : Roy Bicknell
language : en
Publisher: Cambridge University Press
Release Date : 1996-09-28
Endothelial Cell Culture written by Roy Bicknell and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-09-28 with Science categories.
The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Endothelial Cell Culture contains chapters on endothelial cells derived from 1) lung, 2) bone marrow, 3) brain, 4) mammary glands, 5) skin, 6) adipose tissue, 7) female reproductive system, and 8) synovium.
Biology Of Endothelial Cells
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Author : Eric A. Jaffe
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Biology Of Endothelial Cells written by Eric A. Jaffe and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The participation of endothelial cells in various physiologic and pathologic processes has been hypothesized since before the turn of the century. However, until recently, direct evidence for endothelial involvement in these processes has been extremely difficult to obtain due to the inability to study endothelial cell function in vitro. Though the possibility of using cultured endothelial cells to study endothelial cell function in vitro was recognized many years ago, the inability to culture unambiguously identifiable endothelial cells limited investigators in their studies of endothelial function. As a result, the field of endothelial cell biology lay relatively fallow for many years. The development in the early 1970's of routine and easily implemented methods for culturing human endothelial cells and the demonstration that cultured endothelial cells synthesized a physiologically relevant protein, Factor VIII/von Willebrand Factor, quickly changed this state of affairs. Over the following decade the scope of endothelial cell research rapidly widened, spreading in a number of directions. First, methods were developed to culture endothelial cells from a variety of species. Second, methods were developed to culture endothelial cells from different organs and types of blood vessels (arteries, veins, and capillaries) within a single species. Third, and most important, investigators began using cultured endothelial cells as tools to study the potential involvement of endothelial cells in a wide assortment of biologically interesting processes. The net result has been a tremendous increase in our understanding of endothelial cell function.
Introduction To Cell And Tissue Culture
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Author : Jennie P. Mather
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-08-20
Introduction To Cell And Tissue Culture written by Jennie P. Mather and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-20 with Science categories.
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Current Trends In Atherogenesis
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Author : Rita Rezzani
language : en
Publisher: BoD – Books on Demand
Release Date : 2013-02-27
Current Trends In Atherogenesis written by Rita Rezzani and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-02-27 with Medical categories.
This book collects the state of the art of the antioxidants from the clinical and experimental approaches in order to bring a better understanding of the mechanisms and useful therapies for these diseases. We hope that it can indicate new "current trends" for identifying new aspects regarding this scientific problem involving not only anatomical and functional, but also clinical questions.
Neuroimmunology
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Author :
language : en
Publisher: Academic Press
Release Date : 1995-03-01
Neuroimmunology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03-01 with Medical categories.
The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Comprehensive protocols included for the study of: The brain-immune system The neuroimmune system: Effects of the brain on the peripheral immune system Neuroimmune effects from substances of abuse (e.g. cocaine) to hypnosis Measurement of interferons, cytokines, natural killer cells, and major histocompatibility complex molecules Immunohistochemistry methods in the brain Neuropeptides as immunomodulators
Purine And Pyrimidine Metabolism In Man X
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Author : Esther Zoref-Shani
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-01-31
Purine And Pyrimidine Metabolism In Man X written by Esther Zoref-Shani and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-01-31 with Science categories.
th This volume contains articles presented at the X International Symposium on Purines and Pyrimidines in Man, held on May 14 19, 2000 in Tel Aviv, Israel. The first symposium in this series took place in Tel Aviv in 1973. Since then, the symposium has been held every three years in different parts of the world, including Europe, USA and Japan. The participants, in this series of symposia, are characterised by a wide interest in the various aspects of purines and pyrimidines in man, which include biochemistry, genetics, pharmacology, physiology, clinics, etc. Presentations in the symposia include clarification of metabolic pathways, characterisation of enzyme structure and kinetics and discoveries of new inborn errors of metabolism and suggestions for new therapeutic approaches for these inborn errors. In addition, development of new purine and pyrimidine derivatives for the treatment of cancer and viral diseases, and many more subjects of mutual interest were brought to the fore. With the development of therapeutic means and of new research tools, we have witnessed changes in the areas of interest. The interest in gout and uric acid urolithiasis has lessened, whereas molecular aspects, the role of purine and pyrimidine substances in neurotransmission and in purinergic signaling appear to gain greater interest. The articles, included in this volume, contain new data pertaining to the various aspects detailed above.
Basic Cell Culture Protocols
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Author : Cheryl D. Helgason
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Basic Cell Culture Protocols written by Cheryl D. Helgason and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
New Insights Into Cell Culture Technology
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Author : Sivakumar Joghi Thatha Gowder
language : en
Publisher: BoD – Books on Demand
Release Date : 2017-05-10
New Insights Into Cell Culture Technology written by Sivakumar Joghi Thatha Gowder and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-10 with Science categories.
The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]