Biological Fluorescence Imaging On The Nanoscale
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Biological Fluorescence Imaging On The Nanoscale
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Author : Lana Lau
language : en
Publisher:
Release Date : 2013
Biological Fluorescence Imaging On The Nanoscale written by Lana Lau and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
A majority of biological microscopy investigations involve the focusing of visible light with conventional lenses. Fluorescence microscopy is one of the most widely used tools in biology but its resolution has historically suffered from the diffraction limit to about 200 nm laterally and 800 nm axially. In the past decade, this resolution problem has been overcome by the rapidly emerging field of super-resolution microscopy. The first demonstrated super-resolution technique, STimulated Emission Depletion (STED) Microscopy, is the topic of this Dissertation. This Dissertation has two primary areas of focus: the design optimization of a STED microscope, covered in Chapters 2-4, and its application to super-resolution imaging in cells and tissues, covered in Chapters 4-6. Chapter 2 describes the STED apparatus and experimental methods used. This chapter covers the guiding principles behind the design of a STED microscope, which forms a basis for understanding the logic underlying the homebuilt STED microscope which was constructed for this research. This STED microscope has a typical resolution of approximately 60 nm (full-width-at-half-maximum) or 25 nm (sigma) and has the sensitivity to image single fluorophores. In Chapter 3, a framework for evaluating and optimizing STED performance in the presence of several key tradeoffs is presented. Chapter 4 describes both developments in STED Microscopy required to utilize far-red-emitting dyes and the challenges associated with performing super-resolution imaging in intact Drosophila tissue. In Chapter 5, the optimization of labeling density revealed the 9-fold symmetry of a centriole protein structure, an important organelle in cell development. In Chapter 6, Huntingtin protein aggregates are resolved beyond the diffraction limit in a cell model of the neurodegenerative Huntington's disease.
Nanoscale Imaging
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Author : Yuri L. Lyubchenko
language : en
Publisher: Humana
Release Date : 2019-07-13
Nanoscale Imaging written by Yuri L. Lyubchenko and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-13 with Medical categories.
This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.
Label Free Super Resolution Microscopy
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Author : Vasily Astratov
language : en
Publisher: Springer Nature
Release Date : 2019-08-31
Label Free Super Resolution Microscopy written by Vasily Astratov and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-31 with Science categories.
This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.
Far Field Optical Nanoscopy
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Author : Philip Tinnefeld
language : en
Publisher: Springer
Release Date : 2015-02-07
Far Field Optical Nanoscopy written by Philip Tinnefeld and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-02-07 with Science categories.
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Fluorescence Microscopy
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Author : Anda Cornea
language : en
Publisher: Elsevier
Release Date : 2014-02-24
Fluorescence Microscopy written by Anda Cornea and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-24 with Science categories.
Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions
Transparency In Biology
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Author : Kohei Soga
language : en
Publisher: Springer Nature
Release Date : 2020-12-21
Transparency In Biology written by Kohei Soga and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-21 with Science categories.
This book explains transparency in biology with emphasis on bending and absorption, which together are the essence of transparency. The reader is provided with an understanding of why the interior of the body can be made to appear transparent through the application of elementary physics. Based on the principle of transparency, emerging imaging techniques using near-infrared light to view the body transparently are explained with examples such as cancer detection and temperature imaging of deep tissues. This book is useful to many researchers, including biologists, physicists, chemists, materials scientists, and device engineers as well as developers—all who seek a deep understanding of transparency in bioimaging.
Atomic Force Microscopy For Nanoscale Biophysics
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Author : Mi Li
language : en
Publisher: Elsevier
Release Date : 2023-02-15
Atomic Force Microscopy For Nanoscale Biophysics written by Mi Li and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-15 with Science categories.
Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells. The book discusses the methodology of AFM-based biomedical detection, diverse biological systems, and the combination of AFM with other complementary techniques. These state-of-the-art chapters empower researchers to address biological issues through the application of atomic force microscopy. Atomic force microscopy (AFM) is a unique, multifunctional tool for investigating the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution. Summarizes the recent progress of atomic force microscopy in biomedical applications Presents the methods and skills of applying atomic force microscopy Aids researchers in investigating the nanoscale biophysics of diverse biological systems
Biological Responses To Nanoscale Particles
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Author : Peter Gehr
language : en
Publisher: Springer
Release Date : 2019-04-16
Biological Responses To Nanoscale Particles written by Peter Gehr and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-16 with Science categories.
In this book the recent progress accumulated in studies of the interaction of engineered nanoparticles with cells and cellular constituents is presented. The focus is on manufacturing and characterization of nanosized materials, their interactions with biological molecules such as proteins, the mechanisms of transport across biological membranes as well as their effects on biological functions. Fundamental molecular and cellular aspects are in the foreground of the book. A further particularity is the interdisciplinary approach, including fields such as preparatory and analytical chemistry, biophysics and the physics of colloids, advanced microscopy and spectroscopy for in-situ detection of nanoparticles, cellular toxicology and nanomedicine. Nanoscale particles are known to exhibit novel and unprecedented properties that make them different from their corresponding bulk materials. As our ability to control these properties is further advanced, a huge potential to create materials with novel properties and applications emerges. Although the technological and economic benefits of nanomaterials are indisputable, concerns have also been raised that nanoscale structuring of materials might also induce negative health effects. Unfortunately, such negative health effects cannot be deduced from the known toxicity of the corresponding macroscopic material. As a result, there is a major gap in the knowledge necessary for assessing their risk to human health.
Life At The Nanoscale
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Author : Yves Dufrene
language : en
Publisher: CRC Press
Release Date : 2019-03-28
Life At The Nanoscale written by Yves Dufrene and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-28 with Science categories.
Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.
Fluorescence Spectroscopy In Biology
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Author : Martin Hof
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-01-27
Fluorescence Spectroscopy In Biology written by Martin Hof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-01-27 with Science categories.
Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.