Label Free Super Resolution Microscopy

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Label Free Super Resolution Microscopy
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Author : Vasily Astratov
language : en
Publisher: Springer Nature
Release Date : 2019-08-31
Label Free Super Resolution Microscopy written by Vasily Astratov and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-31 with Science categories.
This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.
Stimulated Raman Scattering Microscopy
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Author : Ji-Xin Cheng
language : en
Publisher: Elsevier
Release Date : 2021-12-04
Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-04 with Science categories.
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. - Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy - Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics - Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Super Resolution Microscopy
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Author : Udo J. Birk
language : en
Publisher: John Wiley & Sons
Release Date : 2017-12-04
Super Resolution Microscopy written by Udo J. Birk and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-04 with Science categories.
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Optical Imaging And Sensing
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Author : Jiang Wu
language : en
Publisher: John Wiley & Sons
Release Date : 2023-08-03
Optical Imaging And Sensing written by Jiang Wu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-08-03 with Technology & Engineering categories.
Optical Imaging and Sensing Understand the future of optical imaging with this cutting-edge guide Optoelectronic devices for imaging and sensing are among the backbones of modern technology. Facilitating the mutual conversion of optical and electrical signals, they have applications from telecommunications to molecular spectroscopy, and their incorporation into photon-involved technologies is only growing. The rapid development of this field makes the need for a fully up-to-date introduction all the more critical. Optical Imaging and Sensing meets this need with a comprehensive guide to the novel materials and devices employed in optical imaging and sensing. Given the current revolution in new imaging materials, an introduction that fully incorporates the latest research is an indispensable tool for scientists and engineers in a huge range of fields. The technologies surveyed here promise to transform public security, 5G and next-generation wireless communication, clinical imaging, and many more. Optical Imaging and Sensing Readers will also find: Detailed discussion of materials including semimetallic graphene, semiconducting black phosphorous, and many more Discussion of devices from infrared photodetectors to nonlinear interferometers A thorough look forward to the future of the field Optical Imaging and Sensing is a useful reference for materials scientists, spectroscopists, semiconductor physicists, and engineers working in any field or industry involving optical imaging or sensing technology.
Advanced Imaging And Bio Techniques For Convergence Science
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Author : Jun Ki Kim
language : en
Publisher: Springer Nature
Release Date : 2021-04-08
Advanced Imaging And Bio Techniques For Convergence Science written by Jun Ki Kim and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-08 with Medical categories.
This book is a wide-ranging guide to advanced imaging techniques and related methods with important applications in translational research or convergence science as progress is made toward a new era in integrative healthcare. Conventional and advanced microscopic imaging techniques, including both non-fluorescent (i.e., label-free) and fluorescent methods, have to date provided researchers with specific and quantitative information about molecules, cells, and tissues. Now, however, the different imaging techniques can be correlated with each other and multimodal methods developed to simultaneously obtain diverse and complementary information. In addition, the latest advanced imaging techniques can be integrated with non-imaging techniques such as mass spectroscopic methods, genome editing, organic/inorganic probe synthesis, nanomedicine, and drug discovery. The book will be of high value for researchers in the biological and biomedical sciences or convergence science who need to use these multidisciplinary and integrated techniques or are involved in developing new analytical methods focused on convergence science.
Super Resolution Microscopy
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Author : Holger Erfle
language : en
Publisher: Humana Press
Release Date : 2017-09-19
Super Resolution Microscopy written by Holger Erfle and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-19 with Medical categories.
This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.
The Photonic Hook
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Author : Oleg V. Minin
language : en
Publisher: Springer Nature
Release Date : 2021-03-31
The Photonic Hook written by Oleg V. Minin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-03-31 with Science categories.
This book describes the recently-discovered artificially curved light beam known as the photonic hook. Self-bending of light, a long-time goal of optical scientists, was realized in 2007 with the Airy beam, followed by the first demonstration of the photonic hook by the authors of this book and their collaborators in 2015 and experimentally in 2019. The photonic hook has curvature less than the wavelength, along with other unique features described in this book that are not shared by Airy-like beams, and so deepens our understanding of light propagation. This book discusses the general principles of artificial near-field structured curved light and the full-wave simulations of the photonic hook along with their experimental confirmation. The book goes on to show how the photonic hook has implications for acoustic and surface plasmon waves and as well as applications in nanoparticle manipulation.
Super Resolution Optical Imaging And Microscopy
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Author : Junle Qu
language : en
Publisher: John Wiley & Sons
Release Date : 2023-12-11
Super Resolution Optical Imaging And Microscopy written by Junle Qu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12-11 with Science categories.
Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.
Atomic And Molecular Spectroscopy
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Author : Sune Svanberg
language : en
Publisher: Springer Nature
Release Date : 2023-01-06
Atomic And Molecular Spectroscopy written by Sune Svanberg and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-06 with Science categories.
A wide-ranging review of modern spectroscopic techniques such as X-ray, photoelectron, optical and laser spectroscopy, and radiofrequency and microwave techniques. On the fundamental side the book focuses on physical principles and the impact of spectroscopy on our understanding of the building blocks of matter, while in the area of applications particular attention is given to those in chemical analysis, photochemistry, surface characterisation, environmental and medical diagnostics, remote sensing and astrophyscis. The Fourth Edition also provides the reader with an update on laser cooling and trapping, Bose-Einstein condensation, ultra-fast spectroscopy, high-power laser/matter interaction, satellite-based astronomy and spectroscopic aspects of laser medicine.