Biophysical Characterization Of Functional Peptides
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Biophysical Characterization Of Functional Peptides
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Author : Vibin Ramakrishnan
language : en
Publisher: Springer Nature
Release Date : 2023-08-02
Biophysical Characterization Of Functional Peptides written by Vibin Ramakrishnan and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-08-02 with Science categories.
This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.
Biophysical Characterization Of Bioactive Peptide Amphiphiles
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Author : Ayala Fishel
language : en
Publisher:
Release Date : 2000
Biophysical Characterization Of Bioactive Peptide Amphiphiles written by Ayala Fishel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Peptides categories.
Biophysical Characterization Of Intrinsically Disordered Peptides Through Molecular Dynamics Simulations And Solution Nuclear Magnetic Resonance Spectroscopy
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Author : David Jacob Rosenman
language : en
Publisher:
Release Date : 2015
Biophysical Characterization Of Intrinsically Disordered Peptides Through Molecular Dynamics Simulations And Solution Nuclear Magnetic Resonance Spectroscopy written by David Jacob Rosenman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Collagen Mimetic Peptides And Their Biophysical Characterization
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Author : Jianxi Xiao
language : en
Publisher: Springer
Release Date : 2024-09-21
Collagen Mimetic Peptides And Their Biophysical Characterization written by Jianxi Xiao and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-09-21 with Science categories.
This book embarks on an enlightening journey through the molecular landscape of collagen mimic peptides, shedding light on their significance and potential. In the intricate realm of biomolecules, collagen's preeminence as the most abundant protein in the human body, forming the foundational scaffolding of tissues and organs, renders it a subject of profound scientific interest. It explores diverse facets related to collagen mimic peptides, spanning aspects such as bioinformatic analysis, synthetic strategies, pathological collagen targeting, and the construction of homotrimeric, heterotrimeric and self-assembled peptide models. Furthermore, it provides an exhaustive investigation into biophysical techniques, encompassing Circular Dichroism (CD), X-ray crystallography, Nuclear Magnetic Resonance (NMR), Fluorescence, and Raman spectroscopy, thereby empowering researchers to unravel the structural intricacies of these peptides. The book unravels the profound implications of collagen mimic peptides across a spectrum of scientific domains, including protein science, biomaterials, bioanalysis, and beyond. Its accessibility and insights cater not only to seasoned researchers but also to undergraduate and graduate students eager to delve into the complexities of this fundamental protein.
Biophysical Characterisation Of Peptides And Proteins
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Author : Angeliki Damianoglou
language : en
Publisher:
Release Date : 2010
Biophysical Characterisation Of Peptides And Proteins written by Angeliki Damianoglou and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
Structural And Biophysical Characterization Of Photoswitchable Peptides And Their Complexes
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Author : Piotr Wysoczanski
language : en
Publisher:
Release Date : 2012
Structural And Biophysical Characterization Of Photoswitchable Peptides And Their Complexes written by Piotr Wysoczanski and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Networks of biomacromolecular interaction form the core of internal cellular logic where a wide range of input signals get analysed and results in a genetically programmed output. This description holds not only for healthy cells but is true also for those virally infected when a virus implements new biomacromolecular elements and modifies existing networks. Those hostile interactions are crucial for the HIV virus replication cycle. Due to the scale-free nature of biomacromolecular interaction networks they are prone to malfunction if a hub (node with the highest interactions) is damaged. Perhaps the best example of such a situation can be observed in the apoptotic machinery. Overexpression, deletion or mutations (depending on the context) in apoptotic proteins can lead to various diseases, most prominently cancer. Therefore current research efforts concentrate on designing inhibitors of malfunctioning interactions. Occasionally interaction between the two binding partners relies on a well-defined secondary structures element. This allows to engineer this element and use it to bias the given interaction equilibrium towards dissociation. One such approach concentrates on [alpha]-helices that are crosslinked with azobenzene derivatives. This permits not only to increase the helical content of a peptide, which translates to increased affinity (potency), but transplants a photo-controllability. Photoswitchable peptides created in this way can be controlled externally; their affinity towards targets can be effectively photomodulated. The first chapter of the thesis concentrates on the development and evaluation of photocontrollable peptides derived from the arginine rich region of the HIV type I Rev protein. Rev, which is an early product generated from the HIV genome, binds to RRE containing mRNA and removes it from the nucleus, allowing expression of unspliced or singly spliced mRNA into different protein products. As a consequence the RRE-Rev interaction controls the infection cycle of the HIV type 1, as these later products are necessary for virus particle assembly. The [alpha]-helical peptide derived from Rev was shown to retain most of the affinity to RRE. Rev-RRE serves as a prototypical protein-RNA interaction; a model for the development of the first photo-controllable peptide that targets RNA. This research presents two approaches for the peptide production: chemical synthesis and biosynthesis (overexpression of the Rev peptide gene). Initially the water soluble azobenzene derivative was used bearing two negatively charged sulphonate substituents on the aromatic rings. Surprisingly, the first generation of the peptides had substantially decreased affinity to RRE VI as compared to the wild type Rev peptide. By applying computational chemistry techniques a possible rationalisation was found: sulphonates interact strongly with crucial arginines on the peptide making them unavailable to engage the phosphate backbone; additionally the peptide was forced into non-helical conformation prior to binding. This hypothesis was validated by crosslinking Rev peptides with azobenzene derivative deprived of the sulphonates. The second generation of Rev peptides binds RRE with high affinity and the interaction in question can be controlled effectively by light. The second chapter of the thesis focuses on the structural characterization of a photocontrollable Bak peptide bound to Bcl-xL - a prominent member of the Bcl-2 family of proteins. The Bcl-2 family of proteins includes the major regulators and effectors of the intrinsic apoptosis pathway. Cancers are frequently formed when activation of the apoptosis mechanism is compromised either by misregulated expression of prosurvival family members or, more frequently, by damage to the regulatory pathways that trigger intrinsic apoptosis. Short peptides derived from the pro-apoptotic members of the Bcl-2 family can activate mechanisms that ultimately lead to cell death. The recent development of photocontrolled peptides that are able to change their conformation and activity upon irradiation with an external light source has provided new tools to target cells for apoptosis induction with temporal and spatial control. In this thesis the first NMR solution structure of a photoswitchable peptide derived from the proapoptotic protein Bak in complex with the antiapoptotic protein Bcl-xL is reported. This structure provides insight into the molecular mechanism, by which the increased affinity of such photopeptides compared to their native forms, is achieved, and offers a rationale for the large differences in the binding affinities between the helical and nonhelical states.
Protection Of Functional Groups In Peptide Synthesis
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Author : Erhard Gross
language : en
Publisher: Elsevier
Release Date : 2014-05-10
Protection Of Functional Groups In Peptide Synthesis written by Erhard Gross and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-10 with Science categories.
The Peptides: Analysis, Synthesis, Biology, Volume 3: Protection of Functional Groups in Peptide Synthesis focuses on protection of functional groups in peptide synthesis. This book consists of seven chapters. Chapter 1 reviews the large variety of amine protecting groups. The protection of carboxyl groups is described in Chapter 2, while the chemistry of sulfhydryl group protection in peptide synthesis is discussed in Chapter 3. Chapter 4 covers the protection of the hydroxyl groups of serine, threonine, tyrosine, and other hydroxyl-containing amino acids. Differential protection and selective deprotection in peptide synthesis is deliberated in Chapter 5. In chapter 6, the opportunities and constraints of the tactics of minimal protection of side-chain functions during peptide synthesis are reviewed. The last chapter is devoted to the interesting aspects of dual function groups. This volume is recommended for specialists and researchers concerned with peptide and protein research.
Towards A Better Understanding Of Membrane Proteins
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Author : T. Meijneke
language : en
Publisher:
Release Date : 2010
Towards A Better Understanding Of Membrane Proteins written by T. Meijneke and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
Synthesis And Biophysical Characterisation Of Cationic Amphipathic Ph Responsive Gene Delivery Peptides Containing Primary Amine 2 3 Diaminopropionic Acid Residues And Secondary Amine Mono Methylated Derivatives
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Author : Jayneil Patel
language : en
Publisher:
Release Date : 2011
Synthesis And Biophysical Characterisation Of Cationic Amphipathic Ph Responsive Gene Delivery Peptides Containing Primary Amine 2 3 Diaminopropionic Acid Residues And Secondary Amine Mono Methylated Derivatives written by Jayneil Patel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Gene therapy categories.
Delivery of therapeutic oligonucleotide sequences by cationic amphipathic histidine rich peptides like LAH4-L1 is modulated by peptide response to pH changes during endocytosis. pH sensitivity can be affected by the number of histidine residues, defining its transfection efficiency. Optimizing the gene delivery efficiencies of LAH4-L1 by increasing the number of histidine residues and therefore peptide charge is limited by the Coulombic interactions that stabilise a-helical self-associated peptide oligomers. Consequently, peptides containing six histidine residues function at a pH below that which occurs in the endosome, decreasing gene delivery capabilities. This limitation is expected to be overcome by substituting histidine residues with the more basic pH response 2,3- diaminopropionic acid (Dap). It is predicted that peptides containing 6 Dap residues will remain sensitive to pH changes observed in the endosome, leading to more efficient gene delivery peptide. To test these hypothesis peptides containing four and six Dap residues were synthesised. Analogously, derivatives containing mono-methylated P-amine Dap residues were formed in attempt to reduce cytotoxicity associated with primary amine peptides. Biophysical analysis was performed to determine the peptides pH response in key mechanisms that occur during gene delivery. It is shown that Dap residues can balance the Coulombic effects and respond at a more basic pH than histidine containing analogues. Whereas Dap substitution does not give increased gene delivery compared to LAH4-L1; mono-methylated Dap variants remain sensitive to pH changes in endocytosis and show increased gene delivery efficiencies compared to LAH4-L1. Peptides containing six mono-methylated Dap residues gave transfection results reproducible in serum greater than LAH4-L1 and comparable to commercially available peptide vectors.
Rational Development And Biophysical Analysis Of Medically Relevant Peptides
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Author : Erika J. Olson
language : en
Publisher:
Release Date : 2017
Rational Development And Biophysical Analysis Of Medically Relevant Peptides written by Erika J. Olson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with Peptides categories.