Cell Culture In The Neurosciences
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Cell Culture In The Neurosciences
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Author : Jane Bottenstein
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cell Culture In The Neurosciences written by Jane Bottenstein and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
Neuroscience Approached Thru Cell Culture
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Author : Steven E. Pfeiffer
language : en
Publisher: Springer
Release Date : 1982-08-30
Neuroscience Approached Thru Cell Culture written by Steven E. Pfeiffer and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982-08-30 with Medical categories.
Neuroscience Approached Through Cell Culture
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Author : Steven E. Pfeiffer
language : en
Publisher:
Release Date : 1982
Neuroscience Approached Through Cell Culture written by Steven E. Pfeiffer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Cell culture categories.
Cell Culture In The Neurosciences
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Author : Jane Bottenstein
language : en
Publisher:
Release Date : 1985-05-01
Cell Culture In The Neurosciences written by Jane Bottenstein and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-05-01 with categories.
Protocols For Neural Cell Culture
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Author : Sergey Fedoroff
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-06-29
Protocols For Neural Cell Culture written by Sergey Fedoroff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-29 with Medical categories.
The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.
Cell Culture
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Author : P. Michael Conn
language : en
Publisher:
Release Date : 1990
Cell Culture written by P. Michael Conn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Science categories.
The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline. Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * Preparation and maintenance of primary and continuous cultures * Cell purification * Bulk isolation * Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures * Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking * Clonal analysis and lineage analysis
Culturing Nerve Cells
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Author : Gary Banker
language : en
Publisher: MIT Press
Release Date : 1998
Culturing Nerve Cells written by Gary Banker and has been published by MIT Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Anatomy categories.
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Practical Cell Culture Techniques
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Author : Alan A. Boulton
language : en
Publisher: Humana Press
Release Date : 1992-09-09
Practical Cell Culture Techniques written by Alan A. Boulton and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-09-09 with Medical categories.
Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.
Cell Culture Approaches To Invertebrate Neuroscience
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Author : George Lees
language : en
Publisher:
Release Date : 1988
Cell Culture Approaches To Invertebrate Neuroscience written by George Lees and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Science categories.
Methods In Neurosciences Cell Culture
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Author : P. Michael Conn
language : en
Publisher:
Release Date : 1989
Methods In Neurosciences Cell Culture written by P. Michael Conn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Neurology categories.