Culturing Nerve Cells
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Culturing Nerve Cells
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Author : Gary Banker
language : en
Publisher: MIT Press
Release Date : 1998
Culturing Nerve Cells written by Gary Banker and has been published by MIT Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Anatomy categories.
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Neuronal Cell Culture
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Author : Shohreh Amini
language : en
Publisher: Humana
Release Date : 2022-06-09
Neuronal Cell Culture written by Shohreh Amini and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-09 with Medical categories.
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Developmental Biology Of Cultured Nerve Muscle And Glia
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Author : David Schubert
language : en
Publisher: Wiley-Interscience
Release Date : 1984
Developmental Biology Of Cultured Nerve Muscle And Glia written by David Schubert and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Cell adhesion categories.
The Pharmacology Of Nerve And Muscle In Tissue Culture
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Author : Alan L. Harvey
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Pharmacology Of Nerve And Muscle In Tissue Culture written by Alan L. Harvey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The techniques of tissue culture were introduced at the beginning of this century. They have become more and more popular as it is realized that they are not as difficult or as esoteric as some early protagonists liked to maintain. Most of the work performed with culture methods has simply concerned cell growth and survival. Biologists have long used culture approaches to provide a simple system in which to study cell division and multiplication. Any pharmacology done on cultured tissue was largely toxicological or as part of a screening programme for poten tial anti-cancer drugs. In the last decade there has been a great increase in the use of excitable cells in tissue culture. Nerves and muscles from a wide variety of sources can maintain their highly differentiated properties in culture. Such cultures offer an attractive preparation for use in physiological and pharmacological investigations. Consequently, a vast amount of work has been produced, and this book is an attempt to review it. It is hoped that this will introduce physiologists and pharmacologists to the potential of culture methods for their experiments and also indicate to more traditional tissue culture users further possible areas of interest. By being more comprehensive in scope and by trying to concentrate largely on drug actions, I hope that the present volume usefully extends the treatment of the subject begun earlier in the excellent works by Crain (1976) and Nelson and Lieberman (1981).
Cell Culture In The Neurosciences
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Author : Jane Bottenstein
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cell Culture In The Neurosciences written by Jane Bottenstein and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
Neurons Methods And Applications For The Cell Biologist
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Author : Peter J. Hollenbeck
language : en
Publisher: Gulf Professional Publishing
Release Date : 2003-07-08
Neurons Methods And Applications For The Cell Biologist written by Peter J. Hollenbeck and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-07-08 with Medical categories.
Many cell biologists study the properties of cells grown outside of the animal, in the controlled environment of a culture dish. Many cell biologists would benefit from directing their attention to nerve cells, but are afraid to try growing nerve cells in culture because it has the reputation for being much more difficult and unreliable than growing other kinds of cells. This volume is written by cell biologists and neuroscientists who are experts at growing various kinds of neurons in culture and using them for diverse experimental applications. The aim of this volume is to demystify the techniques used to grow nerve cells and to show cell biologists that it is something that they can easily master. * Contains culture methodology for more than a dozen types of CNS and PNS neurons * Includes most recent and reliable techniques from expert practitioners for specific experimental applications * Addresses the latest strategies for transfecting neurons.
Protocols For Neural Cell Culture
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Author : Sergey Fedoroff
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Protocols For Neural Cell Culture written by Sergey Fedoroff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Medical categories.
This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.
Neuronal Cell Culture
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Author : Shohreh Amini
language : en
Publisher:
Release Date : 2013
Neuronal Cell Culture written by Shohreh Amini and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Cell culture categories.
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
Tissue Culture Of The Nervous System
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Author : Gordon Sato
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Tissue Culture Of The Nervous System written by Gordon Sato and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Medical categories.
The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad vantages of these cultures.
Development Of Cerebellar Nerve Cells In Tissue Culture
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Author : R. Balázs
language : en
Publisher:
Release Date : 1984
Development Of Cerebellar Nerve Cells In Tissue Culture written by R. Balázs and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with categories.