Cell Immortalization
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Cell Immortalization
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Author : Alvaro Macieira-Coelho
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Cell Immortalization written by Alvaro Macieira-Coelho and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Science categories.
The problem of the long-term proliferation of cells is a seminal one. It has always been a hot subject in biology, a source of far-reaching hypotheses, even more so now when explanations for the mechanisms of cell prolifera tive mortality or immortality seem within our reach. A question which is still debated is whether an infinite division potential can be a normal trait or is always the result of modifications leading to abnormal cell growth and escape from homeostasis. In general, investigators have been advocates of one of the two extremes, universal limited or unlim ited normal proliferative potential. Since the long-term proliferative potential of cells concerns regulation of development, regeneration of tissues, and homeostatic control of cell growth, in brief survival of living organisms, and since the regulation of these processes is so different along the evolutionary scale, it is not surpris ing that there does not seem to be any universal trait. The question of whether cells are endowed with finite or infinite prolifera tive phenotypes has to be seen using the perspective of comparative biology.
Culture Of Immortalized Cells
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Author : R. Ian Freshney
language : en
Publisher: Wiley-Liss
Release Date : 1996-04-15
Culture Of Immortalized Cells written by R. Ian Freshney and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-04-15 with Medical categories.
This is a detailed, yet concise manual which provides the most up-to-date in vitro methods & safety procedures for the immortalization of primary cultures for various cell types. Each method is accompanied by step by step protocols & instructions.
Different Combinations Of Genetic Alterations Lead To Human Uroepithelial Cell Immortalization
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Author : Cassandra D. Belair
language : en
Publisher:
Release Date : 1997
Different Combinations Of Genetic Alterations Lead To Human Uroepithelial Cell Immortalization written by Cassandra D. Belair and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
The Identification Of Cellular Genes Altered By Htert And Hpv 16 In The Immortalization Of Human Keratinocytes
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Author : Jonathan J. Miller
language : en
Publisher:
Release Date : 2012
The Identification Of Cellular Genes Altered By Htert And Hpv 16 In The Immortalization Of Human Keratinocytes written by Jonathan J. Miller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Biology categories.
Studies have shown that wild-type hTERT protein can functionally replace the HPV-16E6 protein, which cooperates with the viral E7 protein in the immortalization of primary keratinocytes. Previously, we made the surprising finding that catalytically inactive hTERT (hTERTci), elongation-defective hTERT (hTERT-HA), and telomere recruitment-defective (hTERT N+T) also cooperate with E7 in cell immortalization, indicating that hTERT has immortalizing activities independent of its telomere maintenance functions. Since reports show an hTERT role in gene activation, we performed microarray studies to discover that E6, hTERT and hTERT mutated proteins altered the expression of highly overlapping sets of cellular genes. Pursuing in-depth studies of these targets shared by E6 and hTERT, we focused on AIB1, a nuclear coactivator known to be elevated in some cancers, and BMI1, the core subunit of the Polycomb Group Repressor Complex (PRC) 1 which is known to play a role in immortalization and determining cell fate. We proved that AIB1 levels were increased in a number of cervical cancer cell lines. Additionally, both AIB1 and BMI1 are elevated in HPV immortalized cell lines. We showed further that BMI1 can substitute for E6 or hTERT in cell immortalization. Finally, in vivo tissue studies revealed expression of AIB1 and BMI1 increase with the severity of cervical dysplasia, suggesting a potential role in cervical cancer. Whereas BMI1 appears to be a marker of progression differentiating between pre-neoplastic and neoplastic lesions, AIB1 appears to delineate invasion from earlier stages, significantly increasing in invasive carcinoma. Together, these data demonstrate that AIB1 may be a novel cellular target of the HPV E6 oncoprotein and that hTERT has extratelomeric activities in cell immortalization and that its induction of BMI1 is a potential mechanism for mediating this activity.
Cell Culture
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Author : Alan Doyle
language : en
Publisher:
Release Date : 1997-07-04
Cell Culture written by Alan Doyle and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-07-04 with Medical categories.
This book provides concise practical instructions for setting up a cell culture facility and operating it effectively and safely. Procedures are described to guide the readers from the initiation of primary cultures, handling established cell lines and quantifying their performance, through to the production of specialist cell types by immortalization. The user-friendly format makes Mammalian Cell Culture: Essential Techniques a convenient summary of techniques for experienced workers as well as an invaluable aid to tissue culture in nonspecialized environments. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
Animal Cell Biotechnology
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Author : Nigel Jenkins
language : en
Publisher: Humana Press
Release Date : 1999-02-22
Animal Cell Biotechnology written by Nigel Jenkins and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-02-22 with Science categories.
Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.
Generation Of Antibodies By Cell And Gene Immortalization
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Author : Cox Terhorst
language : en
Publisher: S. Karger AG (Switzerland)
Release Date : 1993
Generation Of Antibodies By Cell And Gene Immortalization written by Cox Terhorst and has been published by S. Karger AG (Switzerland) this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medical categories.
The Role Of Telomere Length And Telomerase Activity In Cell Immortalization And Tumourigenesis Microform
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Author : Christopher Morris Counter
language : en
Publisher: National Library of Canada = Bibliothèque nationale du Canada
Release Date : 1996
The Role Of Telomere Length And Telomerase Activity In Cell Immortalization And Tumourigenesis Microform written by Christopher Morris Counter and has been published by National Library of Canada = Bibliothèque nationale du Canada this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with categories.
Immortalization And Transformation Of Syrian Hamster Pancreatic Duct Cell Cultures By Sv40 Large T Antigen
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Author : Sergio Laconi
language : en
Publisher:
Release Date : 1994
Immortalization And Transformation Of Syrian Hamster Pancreatic Duct Cell Cultures By Sv40 Large T Antigen written by Sergio Laconi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.
Cell Biology
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Author : Julio E. Celis
language : en
Publisher: Elsevier
Release Date : 2006
Cell Biology written by Julio E. Celis and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Science categories.
This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) Organelle and Cellular Structures, Assays (Volume 2) Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) Indispensable bench companion for every life science laboratory Provides the latest information on the plethora of technologies needed to tackle complex biological problems Includes numerous illustrations, some in full color, supporting steps and results