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Characterization Of Saccharomyces Cerevisiae Transcription Elongation Factor Tfiis By Dna Microarray And Two Hybrid Analysis


Characterization Of Saccharomyces Cerevisiae Transcription Elongation Factor Tfiis By Dna Microarray And Two Hybrid Analysis
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Characterization Of Saccharomyces Cerevisiae Transcription Elongation Factor Tfiis By Dna Microarray And Two Hybrid Analysis


Characterization Of Saccharomyces Cerevisiae Transcription Elongation Factor Tfiis By Dna Microarray And Two Hybrid Analysis
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Author : Christopher Warren Seidel
language : en
Publisher:
Release Date : 2002

Characterization Of Saccharomyces Cerevisiae Transcription Elongation Factor Tfiis By Dna Microarray And Two Hybrid Analysis written by Christopher Warren Seidel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.




Genetic And Genomic Analyses Of The Saccharomyces Cerevisiae Transcript Elongation Factor Tfiis


Genetic And Genomic Analyses Of The Saccharomyces Cerevisiae Transcript Elongation Factor Tfiis
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Author : Caren Jody Stark
language : en
Publisher:
Release Date : 2003

Genetic And Genomic Analyses Of The Saccharomyces Cerevisiae Transcript Elongation Factor Tfiis written by Caren Jody Stark and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with categories.




Dissertation Abstracts International


Dissertation Abstracts International
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Author :
language : en
Publisher:
Release Date : 2003

Dissertation Abstracts International written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Dissertations, Academic categories.




Biochemical And Genetic Characterization Of The Transcription Elongation Factor Tfiis From The Yeast Saccharomyces Cerevisiae


Biochemical And Genetic Characterization Of The Transcription Elongation Factor Tfiis From The Yeast Saccharomyces Cerevisiae
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Author : Karen Renee Christie
language : en
Publisher:
Release Date : 1995

Biochemical And Genetic Characterization Of The Transcription Elongation Factor Tfiis From The Yeast Saccharomyces Cerevisiae written by Karen Renee Christie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.


Regulation of the process of transcriptional elongation is an important control mechanism in the expression of some genes. To fully understand this form of regulation will require better understanding of the functions of transcription elongation factors. The goal of this work was to characterize the transcription elongation factor TFIIS from Saccharomyces cerevisiae, originally called P37. I demonstrated that, like the mammalian TFIIS proteins, the yeast protein stimulates RNA polymerase II to cleave the nascent RNA transcript and to read-through an intrinsic block to elongation. Investigation of the protein-protein contacts between TFIIS and RNA polymerase II indicated that the carboxyl-terminal domain of the largest subunit, subunit four, and subunit seven of the polymerase are not required for TFIIS to promote cleavage and read-through by the polymerase. In addition the carboxyl-terminal half of the yeast TFIIS protein is sufficient for both of these in vitro activities. This result is consistent with the previous results demonstrating the carboxyl-terminus of mouse TFIIS was sufficient to activate RNA polymerase in vitro.



Genetic Characterization Of The Transcript Elongation Factor Tfiis From Saccharomyces Cerevisiae


Genetic Characterization Of The Transcript Elongation Factor Tfiis From Saccharomyces Cerevisiae
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Author : Judith Kimberly Davie
language : en
Publisher:
Release Date : 1998

Genetic Characterization Of The Transcript Elongation Factor Tfiis From Saccharomyces Cerevisiae written by Judith Kimberly Davie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.




Biochemical And Genetic Characterization Of The Transcription Elongation Factor Tfiis From The Yeast Saccharomyces Servistae


Biochemical And Genetic Characterization Of The Transcription Elongation Factor Tfiis From The Yeast Saccharomyces Servistae
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Author : Karen Renee Christie
language : en
Publisher:
Release Date : 1995

Biochemical And Genetic Characterization Of The Transcription Elongation Factor Tfiis From The Yeast Saccharomyces Servistae written by Karen Renee Christie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.




Analysis Of Dna Binding Proteins In Yeast Saccharomyces Cerevisiae


Analysis Of Dna Binding Proteins In Yeast Saccharomyces Cerevisiae
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Author : Su-Wen Ho
language : en
Publisher:
Release Date : 2010

Analysis Of Dna Binding Proteins In Yeast Saccharomyces Cerevisiae written by Su-Wen Ho and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Electronic dissertations categories.


Gene expression is an elaborate and finely tuned process involving the regulated interactions of multiple proteins with promoter and enhancer elements. A variety of approaches are currently used to study these interactions in vivo, in vitro as well as in silico. With the genome sequences of many organisms now readily available, a plethora of DNA functional elements have been predicted, but the process of identifying the proteins that bind to them in vivo remains a bottleneck. I developed two high-throughput assays to address this issue. The first is a modification of the yeast "one-hybrid" assay. The second is probing protein microarrays with DNA sequence elements. Using these methods, I identified two proteins, Sef1 and Yjl103c, that bind to the same DNA sequence element. Sef1 and Yjl103c are little-characterized members of the zinc cluster family of transcription factors of S. cerevisiae. Characterization of their mechanism of action as well as identification of some of their target genes leads to the conclusion that they play a pivotal role in the transcriptional regulation of utilization of nonfermentable carbon sources by budding yeast.



Characterization Of The Transcription Elongation Factors Elf1 And Tfiis In S Cerevisiae


Characterization Of The Transcription Elongation Factors Elf1 And Tfiis In S Cerevisiae
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Author : Donald Prather
language : en
Publisher:
Release Date : 2005

Characterization Of The Transcription Elongation Factors Elf1 And Tfiis In S Cerevisiae written by Donald Prather and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Genetic transcription categories.




Thermodynamic And Kinetic Characterization Of The Interaction Of Saccharomyces Cerevisiae Tata Binding Protein And Transcription Factor Tfiib With Dna Promoters


Thermodynamic And Kinetic Characterization Of The Interaction Of Saccharomyces Cerevisiae Tata Binding Protein And Transcription Factor Tfiib With Dna Promoters
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Author : Victoria Petri
language : en
Publisher:
Release Date : 1998

Thermodynamic And Kinetic Characterization Of The Interaction Of Saccharomyces Cerevisiae Tata Binding Protein And Transcription Factor Tfiib With Dna Promoters written by Victoria Petri and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.




Transcription Elongation Factor Tfiis In Schizosaccharomyces Pombe


Transcription Elongation Factor Tfiis In Schizosaccharomyces Pombe
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Author : Laura Alicia Williams
language : en
Publisher:
Release Date : 1994

Transcription Elongation Factor Tfiis In Schizosaccharomyces Pombe written by Laura Alicia Williams and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.