Chemical And Enzymatic Synthesis Of Gene Fragments
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Chemical And Enzymatic Synthesis Of Gene Fragments
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Author : Hans Günter Gassen
language : en
Publisher:
Release Date : 1982
Chemical And Enzymatic Synthesis Of Gene Fragments written by Hans Günter Gassen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Cell cycle categories.
Preface: In the past the chemical and enzymatic synthesis of oligonucleotides of defined sequence had to be left to a few experts. Now, however, with the triester approach, the phosphite method and the solid-support techniques gene fragment synthesis has turned into an easy procedure even for a non-chemist. Due to the elegant chemistry involved, all methods work without sophisticated equipment and are prone to mechanisation and eventual automation. It is hoped that combined chemical-encymatic gene synthesis may become a standard technique in a molecular biology laboratory, such as DNA sequencing or in-vitro recombination of nucleic acides. We omitted chemical RNA synthesis, since this field is developing so rapidly at the moment that one has to refer to the original publications. However, we included enzymatic synthesis of RNA fragments, procedures which already have obtained a high degree of standardisation. Most of the contributions are revised versions of the protocols supplied for the EMBO sponsored course on "Automated Chemical and Enzymic Gene Synthesis", held in Darmstadt, March 21 to April 3, 1982. The protocols were improved on the basis of the experience of 30 student scientists with chemical, biological or medical backgrounds. Previously omitted procedures, such as the wandering spot method for oligonucleotide analysis, were included. In editing the manuscript we encountered problems with the nomenclature of nucleic acid components. In unambiquous cases we favoured a simple description, hoping for example, that oligodeoxynucleotide is always understood to mean oligo-2'-deoxyribonucleotide. This book aims to provide those interested in DNA/RNA research with state-of-the-art methods in the synthesis, purification, and analysis of DNA and RNA fragments. The editors wish to thank the authors for their efforts in preparing manuscripts from the the revised laboratory protocols. We gratefully acknowledge the skill and the patience of Mrs. E. Ronnfeldt in typing the manuscripts. We express our thanks to Verlag Chemie for the friendly and very efficient cooperation.--H.G. Gassen A. Lang--Darmstadt, in July 1982.
Synthesis And Applications Of Dna And Rna
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Author : Saran Narang
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Synthesis And Applications Of Dna And Rna written by Saran Narang and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Synthesis and Applications of DNA and RNA discusses the significant contributions in the development of synthetic routes to DNA and RNA. This book contains nine chapters that describe the complexities in the chemistry and biology of DNA and RNA. After briefly dealing with the various stages of development in the chemical synthesis of polynucleotides, this book goes on presenting the DNA synthesis on solid supports and through the phosphoramidite method on silica supports. The discussions then shift to the chemical-enzymatic synthesis of expressed genes; the biochemical aspects of chemical syntheses of oligoribonucleotides; and the methods of rapid DNA and RNA sequence analysis. A chapter specifically tackles the protocols of DNA synthesis using double-stranded plasmid DNA as a template. The final chapter deals with the use of oligonucleotides for the identification and isolation of specific gene sequences. This chapter also covers the use oligonucleotides in the detection of human genetic diseases. Biologists, geneticists, and researchers interested in DNA and RNA synthesis will find this work invaluable.
Enzymatic Synthesis Of Dna
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Author : Arthur Kornberg
language : en
Publisher:
Release Date : 1961
Enzymatic Synthesis Of Dna written by Arthur Kornberg and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1961 with DNA. categories.
Chemical Synthesis In Molecular Biology
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Author : Helmut Blöcker
language : en
Publisher:
Release Date : 1987
Chemical Synthesis In Molecular Biology written by Helmut Blöcker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Molecular biology categories.
Protein-DNA recognition: The ineraction of lac. cl and E.coli RNA plymerase with Operators and promotors 1. Rapide solid-phase phosphotriester synthesis of DNA fragments on controlled pore glass and appçication to the preparation of a gene for somatomedin C 13. Cleavage of phosphorothioate-containing oligonucleotides and DNA by restriction endonucleases. Regio-and stereospecific synthesis of 7-Deazapurine 2 - Deoxyribonucleosides and incorporation of nucleooside isosteres into oligonucleotides. Repair-resistant nucleoside analogues in oligonucleotide-directed mutagenesis. Total synthesis of yeast alanyl tRNA. Synthesis of 5-Azacytosine 2'-Deoxyribonucleosides, proprerties and biological activity. Synthesis and characterization of a set of four dodecadeoxyribonucleoside Undecaphospates containing 0 - Methylguanine opposite ademine, cytosine, Guanine, and thymine. Chemical synthesis and biological applications of oligodeoxynucleotides containig carcinogen-DNA Adducts: 06-Methylguanine. Biotinylated ologonucleotide hybridization probes: Progress towards a Non-radioactive method for the deiagnosis of human genetic diseases.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Nucleic Acids Synthesis
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Author : Hubert Köster
language : en
Publisher: IRL Press
Release Date : 1980
Nucleic Acids Synthesis written by Hubert Köster and has been published by IRL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Science categories.
Expanding The Scope Of The Enzymatic Synthesis Of Dna For
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Author : Aaron M. Leconte
language : en
Publisher:
Release Date : 2009
Expanding The Scope Of The Enzymatic Synthesis Of Dna For written by Aaron M. Leconte and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with DNA. categories.
DNA polymerases synthesize DNA, the essential biomolecule responsible for encoding the complex information necessary for life, with remarkable efficiency and fidelity. Chemists and biologists have exploited this extraordinary enzyme to develop a wide range of biotechnological tools, including but certainly not limited to PCR and Sanger sequencing. While these landmark applications continue to be relied upon to this day, they represent only a small fraction of the possible uses of these enzymes. Here, I detail my work to expand the scope of the enzymatic synthesis of DNA in two distinct fields. Chapters 2-5 detail efforts to identify replicable candidate unnatural base pairs to expand the genetic alphabet from the natural four base code to an expanded six base code. Using a wide range of techniques, including chemical synthesis, directed evolution of DNA polymerases, and screening based methodologies, the d 5SICS:d MMO2 base pair is identified, resulting in our strongest candidate base pair identified to date. Chapter 6 details the use of an activity based phage display system to identify a DNA polymerase that possesses improved recognition of substrates applicable in next generation sequencing applications.
Cyclic Enzymatic Solid Phase Synthesis Of Dna Oligonucleotides On An Epoxide Activated Resin Electronic Resource
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Author : Ahmed Mirza Khan
language : en
Publisher:
Release Date : 2008
Cyclic Enzymatic Solid Phase Synthesis Of Dna Oligonucleotides On An Epoxide Activated Resin Electronic Resource written by Ahmed Mirza Khan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with DNA categories.
Standard chemical DNA synthesis with isotope labels requires expensive reagents; moreover, a large excess of phosphoramadites (typically 50-100 fold) must be used. We developed a process where enzymatic cyclic solid phase synthesis of DNA allows for more economic reagent use. A DNA template was immobilized on an epoxy-activated solid support. This chemistry was chosen because the formed linkage is inert to high pH conditions. High efficiency of the covalent attachment was observed when the reaction was carried out in MgCl2/CAPS buffer. It was found that Mg2+ enables the reaction to be completed over a period of 14 h, compared to 72 h under standard conditions. DNA synthesis was carried in a cyclic fashion on a support bound DNA using Klenow fragment.
Enzymatic Synthesis Of Chemically Modified Dna For Analytical And Diagnostic Applications
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Author : Xiaomei Ren
language : en
Publisher:
Release Date : 2015
Enzymatic Synthesis Of Chemically Modified Dna For Analytical And Diagnostic Applications written by Xiaomei Ren and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Biosynthesis Of Macromolecules
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Author : Vernon M. Ingram
language : en
Publisher:
Release Date : 1972
Biosynthesis Of Macromolecules written by Vernon M. Ingram and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Biosynthesis categories.