Chemical Ligation
DOWNLOAD
FREE 30 Days
Download Chemical Ligation PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Chemical Ligation book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Chemical Ligation
DOWNLOAD
FREE 30 Days
Author : Luca D. D'Andrea
language : en
Publisher: John Wiley & Sons
Release Date : 2017-03-27
Chemical Ligation written by Luca D. D'Andrea and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-27 with Science categories.
Presenting a wide array of information on chemical ligation – one of the more powerful tools for protein and peptide synthesis – this book helps readers understand key methodologies and applications that protein therapeutic synthesis, drug discovery, and molecular imaging. • Moves from fundamental to applied aspects, so that novice readers can follow the entire book and apply these reactions in the lab • Presents a wide array of information on chemical ligation reactions, otherwise scattered across the literature, into one source • Features comprehensive and multidisciplinary coverage that goes from basics to advanced topics • Helps researchers choose the right chemical ligation technique for their needs
Protein Ligation And Total Synthesis I
DOWNLOAD
FREE 30 Days
Author : Lei Liu
language : en
Publisher: Springer
Release Date : 2015-05-15
Protein Ligation And Total Synthesis I written by Lei Liu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-15 with Science categories.
Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.
Total Chemical Synthesis Of Proteins
DOWNLOAD
FREE 30 Days
Author : Ashraf Brik
language : en
Publisher: John Wiley & Sons
Release Date : 2021-06-08
Total Chemical Synthesis Of Proteins written by Ashraf Brik and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-08 with Science categories.
How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.
Protein Ligation And Total Synthesis Ii
DOWNLOAD
FREE 30 Days
Author : Lei Liu
language : en
Publisher: Springer
Release Date : 2015-05-15
Protein Ligation And Total Synthesis Ii written by Lei Liu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-15 with Science categories.
Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.
Photocleavable Templates For Pna Base Pairing Mediated Native Chemical Ligation
DOWNLOAD
FREE 30 Days
Author : Stephen Middel
language : en
Publisher: Cuvillier Verlag
Release Date : 2016-09-16
Photocleavable Templates For Pna Base Pairing Mediated Native Chemical Ligation written by Stephen Middel and has been published by Cuvillier Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-16 with Science categories.
The modification of proteins is a fundamental task in natural sciences in order to control and to understand biochemical processes. To address more complicated targets and to widen the spectrum of possible modifications, it is necessary to develop new strategies and to expand the established techniques. Herein, a novel PNA base pairing guided ligation strategy applying photocleavable templates is presented. This strategy implies the design of PNA/peptide hybrids, which are connected via a photocleavable linker. Sequence specific assembly of two PNA/peptide hybrids can be achieved by PNA base pairing. Thereby, the reactive centers are brought in close proximity and a ligation between the two hybrids is facilitated. Finally, the guiding units can be cleaved by irradiation with UV light.
Chemical Protein Synthesis
DOWNLOAD
FREE 30 Days
Author : Xuechen Li
language : en
Publisher: Springer Nature
Release Date : 2022-06-27
Chemical Protein Synthesis written by Xuechen Li and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-27 with Science categories.
This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Chemoselective And Bioorthogonal Ligation Reactions
DOWNLOAD
FREE 30 Days
Author : W. Russ Algar
language : en
Publisher: John Wiley & Sons
Release Date : 2017-03-17
Chemoselective And Bioorthogonal Ligation Reactions written by W. Russ Algar and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-17 with Technology & Engineering categories.
This timely, one-stop reference is the first on an emerging and interdisciplinary topic. Covering both established and recently developed ligation chemistries, the book is divided into two didactic parts: a section that focuses on the details of bioorthogonal and chemoselective ligation reactions at the level of fundamental organic chemistry, and a section that focuses on applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and benefits of the ligation reactions. With chapters authored by outstanding scientists who range from trailblazers in the field to young and emerging leaders, this book on a highly interdisciplinary topic will be of great interest for biochemists, biologists, materials scientists, pharmaceutical chemists, organic chemists, and many others.
Organic Reactions
DOWNLOAD
FREE 30 Days
Author :
language : en
Publisher: John Wiley & Sons
Release Date : 2019-01-14
Organic Reactions written by and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-14 with Science categories.
The 97th volume in this series for organic chemists in industry presents critical discussions of widely used organic reactions or particular phases of a reaction. The material is treated from a preparative viewpoint, with emphasis on limitations, interfering influences, effects of structure and the selection of experimental techniques. The work includes tables that contain all possible examples of the reaction under consideration. Detailed procedures illustrate the significant modifications of each method.
Extending The Scope Of Protein Synthesis By A Novel Auxiliary Based Native Chemical Ligation Strategy
DOWNLOAD
FREE 30 Days
Author : Christina Nadler
language : en
Publisher: Cuvillier Verlag
Release Date : 2013-09-18
Extending The Scope Of Protein Synthesis By A Novel Auxiliary Based Native Chemical Ligation Strategy written by Christina Nadler and has been published by Cuvillier Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09-18 with Science categories.
There is a constant need for developing improved methods for introducing artificial functionalities into peptides and proteins, as the modification of peptides and proteins is one of the major routes to investigate biological function in vitro and in vivo, e.g. by introduction of spin labels or fluorophores. To improve the synthetic accessibility of chemically modified peptides and proteins a new cysteine-free Native Chemical Ligation strategy based on a photocleavable auxiliary was developed and successfully implemented. In addition, a novel protocol for labeling peptides and proteins by introducing artificial, histidine-mimicking amino acids was devised. These triazole-based building blocks were utilized for the introduction of additional metal binding sites into peptides as well as for the development of peptidic zinc sensors based on zinc finger peptide Zif268.
Expressed Protein Ligation
DOWNLOAD
FREE 30 Days
Author : Miquel Vila-Perelló
language : en
Publisher: Humana
Release Date : 2021-03-21
Expressed Protein Ligation written by Miquel Vila-Perelló and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-03-21 with Science categories.
This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.