Chemical Strategies For Investigation Of Deubiquitinases
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Chemical Strategies For Investigation Of Deubiquitinases
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Author : Samuel D. Whedon
language : en
Publisher:
Release Date : 2018
Chemical Strategies For Investigation Of Deubiquitinases written by Samuel D. Whedon and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
Regulation of protein structure and function by post-translational modification is a key mechanism in cellular homeostasis. Among known modifications the small protein ubiquitin is unique in the breadth of functions it directs. Regulation of protein ubiquitylation is the function of more than 600 ligases, and ~100 deubiquitinases. Dysregulated ubiquitylation is involved in infection, inflammation, neurodegeneration, metabolic syndromes and cancer. Therapeutic intervention in these conditions benefits from characterization of substrate-specific ligases and deubiquitinases. Substrate specificity is documented among deubiquitinases, but lags behind knowledge of ligases. In the interest of characterizing specificity of cysteine protease deubiquitinases we have developed chemical methods for site-specific ubiquitylation and electrophile incorporation. We began by developing a ubiquitin-derived electrophile through installation of a C-terminal selenocysteine residue, and orthogonal oxidative conversion to dehydroalanine. Upon validation of the electrophile we expanded our substrate scope to a ubiquitylated peptide, with which we captured the deubiquitinase USP15. In order to access ubiquitylated lysine residues in protein regions inaccessible by native chemical ligation we undertook the synthesis of two selenazolidine amino acids for amber suppression. While conducting amber suppression selections we concurrently pursued semi-synthesis of p53, in order to introduce a C-terminal ubiquitin electrophile. Purification of the semi-synthetic protein proved materially intensive, prompting development of cleavable affinity handles for ligation product purification. A strategy for incorporation at glycine and incorporation at glutamine were developed. A glycine-derived biotin handle proved amenable to reduction by Zn, which furnishes a native glycine, and aromatic thiols, which formed novel site-specific conjugates at the previously modified glycine. Incorporation of a pendant flag tag at glutamine enabled immunoprecipitation followed by traceless tag removal with Zn.
Deubiquitinases
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Author : Julie Maupin-Furlow
language : en
Publisher: Springer Nature
Release Date : 2022-11-09
Deubiquitinases written by Julie Maupin-Furlow and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-09 with Science categories.
This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Molecular Studies Of Covid 19 Chemistry
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Author : Emilia Pedone
language : en
Publisher: Frontiers Media SA
Release Date : 2021-10-20
Molecular Studies Of Covid 19 Chemistry written by Emilia Pedone and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-20 with Science categories.
Studies In Natural Products Chemistry
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Author : Atta-ur Rahman
language : en
Publisher: Elsevier
Release Date : 2022-05-17
Studies In Natural Products Chemistry written by Atta-ur Rahman and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-17 with Science categories.
Studies in Natural Products Chemistry, Volume 73 covers the synthesis, testing and recording of medicinal properties of natural products, providing cutting-edge accounts of fascinating developments in the isolation, structure elucidation, synthesis, biosynthesis and pharmacology of a diverse array of bioactive natural products. Natural products in the plant and animal kingdom offer a huge diversity of chemical structures that are the result of biosynthetic processes that have been modulated over millennia through genetic effects. With rapid developments in spectroscopic techniques and accompanying advances in high-throughput screening techniques, it has become possible to isolate and determine the structures and biological activity of natural products. Hence, these new discoveries have created new avenues and applications for their use. Focuses on the chemistry of bioactive natural products Contains contributions by leading authorities in the field Presents sources of new pharmacophores
Studies In Natural Products Chemistry
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Author :
language : en
Publisher: Elsevier
Release Date : 2016-09-06
Studies In Natural Products Chemistry written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-06 with Science categories.
Studies in Natural Products Chemistry: Bioactive Natural Products (Part XII) is the latest in a series that covers the synthesis or testing and recording of the medicinal properties of natural products, providing cutting-edge accounts of the fascinating developments in the isolation, structure elucidation, synthesis, biosynthesis, and pharmacology of a diverse array of bioactive natural products. Natural products in the plant and animal kingdom offer a huge diversity of chemical structures that are the result of biosynthetic processes that have been modulated over the millennia through genetic effects. With the rapid developments in spectroscopic techniques and accompanying advances in high-throughput screening techniques, it has become possible to quickly isolate and determine the structures and biological activity of natural products, thus opening up exciting opportunities in the field of new drug development to the pharmaceutical industry. Focuses on the chemistry of bioactive natural products Contains contributions by leading authorities in the field Presents sources of new pharmacophores
Comprehensive Natural Products Iii
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Author :
language : en
Publisher: Elsevier
Release Date : 2020-07-22
Comprehensive Natural Products Iii written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-22 with Science categories.
Comprehensive Natural Products III, Third Edition, Seven Volume Set updates and complements the previous two editions, including recent advances in cofactor chemistry, structural diversity of natural products and secondary metabolites, enzymes and enzyme mechanisms and new bioinformatics tools. Natural products research is a dynamic discipline at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids and enzymes. This book reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine and to stimulate new ideas among the established natural products community. Provides readers with an in-depth review of current natural products research and a critical insight into the future direction of the field Bridges the gap in knowledge by covering developments in the field since the second edition published in 2010 Split into 7 sections on key topics to allow students, researchers and professionals to find relevant information quickly and easily Ensures that the knowledge within is easily understood by and applicable to a large audience
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation
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Author :
language : en
Publisher: Academic Press
Release Date : 2020-05-29
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-05-29 with Science categories.
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
Chemical Biology Tools For Peptide And Protein Research
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Author : Yu-Hsuan Tsai
language : en
Publisher: Frontiers Media SA
Release Date : 2022-03-14
Chemical Biology Tools For Peptide And Protein Research written by Yu-Hsuan Tsai and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-14 with Science categories.
Protein Degradation With New Chemical Modalities
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Author : Hilmar Weinmann
language : en
Publisher: Royal Society of Chemistry
Release Date : 2020-10-07
Protein Degradation With New Chemical Modalities written by Hilmar Weinmann and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-07 with Medical categories.
Targeting protein degradation using small molecules is one of the most exciting small-molecule therapeutic strategies in decades and a rapidly growing area of research. In particular, the development of proteolysis targeting chimera (PROTACs) as potential drugs capable of recruiting target proteins to the cellular quality control machinery for elimination has opened new avenues to address traditionally ‘difficult to target’ proteins. This book provides a comprehensive overview from the leading academic and industrial experts on recent developments, scope and limitations in this dynamically growing research area; an ideal reference work for researchers in drug discovery and chemical biology as well as advanced students.
Total Chemical Synthesis Of Proteins
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Author : Ashraf Brik
language : en
Publisher: John Wiley & Sons
Release Date : 2021-06-08
Total Chemical Synthesis Of Proteins written by Ashraf Brik and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-08 with Science categories.
How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.