Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation
DOWNLOAD
Download Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2020-05-29
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Optical Imaging And Conjugation written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-05-29 with Science categories.
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Chemical Optical And Bioorthogonal Methods
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2020-07-24
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Chemical Optical And Bioorthogonal Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-24 with Science categories.
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing ß-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods Based On Optical Imaging And Fluorescence
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2020-06-17
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods Based On Optical Imaging And Fluorescence written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-17 with Science categories.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Prokaryotic And Eukaryotic Systems
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2020-05-14
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods For Prokaryotic And Eukaryotic Systems written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-05-14 with Science categories.
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
The Chemistry Of Imaging Probes
DOWNLOAD
Author : Lorenzo Tei
language : en
Publisher: Frontiers Media SA
Release Date : 2018-10-19
The Chemistry Of Imaging Probes written by Lorenzo Tei and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-19 with categories.
Over the past decades, the field of molecular imaging has been rapidly growing involving multiple disciplines such as medicine, biology, chemistry, pharmacology and biomedical engineering. Any molecular imaging procedure requires an imaging probe that is an agent used to visualize, characterize and quantify biological processes in living systems. Such a probe typically consists of an agent that usually produces signal for imaging purpose, a targeting moiety, and a linker connecting the targeting moiety and the signaling agent. Many challenging problems of molecular imaging can be addressed by exploiting the great possibilities offered by modern synthetic organic and coordination chemistry and the powerful procedures provided by conjugation chemistry. Thus, chemistry plays a decisive role in the development of this cutting-edge methodology. Currently, the diagnostic imaging modalities include Magnetic Resonance Imaging (MRI), Computed Tomography (CT), Ultrasound (US), Nuclear Imaging (PET, SPECT), Optical Imaging (OI) and Photoacoustic Imaging (PAI). Each of these imaging modalities has its own advantages and disadvantages, and therefore, a multimodal approach combining two techniques is often adopted to generate complementary anatomical and functional information of the disease. The basis for designing imaging probes for a given application is dictated by the chosen imaging modality, which in turn is dependent upon the concentration and localization profile (vascular, extracellular matrix, cell membrane, intracellular, near or at the cell nucleus) of the target molecule. The development of high-affinity ligands and their conjugation to the targeting vector is also one of the key steps for pursuing efficient molecular imaging probes. Other excellent reviews, text and monographs describe the principles of biomedical imaging, focusing on molecular biology or on the physics behind the techniques. This Research Topic aims to show how chemistry can offer molecular imaging the opportunity to express all its potential.
The Chemistry Of Molecular Imaging
DOWNLOAD
Author : Nicholas Long
language : en
Publisher: John Wiley & Sons
Release Date : 2014-12-01
The Chemistry Of Molecular Imaging written by Nicholas Long and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-12-01 with Technology & Engineering categories.
Molecular imaging is primarily about the chemistry of novelbiological probes, yet the vast majority of practitioners are notchemists or biochemists. This is the first book, written from achemist's point of view, to address the nature of the chemicalinteraction between probe and environment to help elucidatebiochemical detail instead of bulk anatomy. Covers all of the fundamentals of modern imaging methodologies,including their techniques and application within medicine andindustry Focuses primarily on the chemistry of probes and imagingagents, and chemical methodology for labelling andbioconjugation First book to investigate the chemistry of molecularimaging Aimed at students as well as researchers involved in the areaof molecular imaging
Visualizing Chemistry
DOWNLOAD
Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2006-06-01
Visualizing Chemistry written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-06-01 with Science categories.
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Chemical Tools To Perturb And Observe Complex Biology
DOWNLOAD
Author : Mark Anthony Sellmyer
language : en
Publisher:
Release Date : 2010
Chemical Tools To Perturb And Observe Complex Biology written by Mark Anthony Sellmyer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
A detailed understanding of living systems requires tools to examine and manipulate biological processes. Small molecules and optical imaging technologies are uniquely suited for this purpose. Small molecules enable the specific manipulation of biomolecular targets, and optical imaging permits the real-time observation of molecular and cellular processes in vivo. This dissertation describes a combination of chemical tool development and imaging strategies to address the following biological problems: 1) specific modification of the genome 2) exquisite control of protein function 3) observing cell-cell interactions in living animals. Chapter One describes a technology for targeted gene modification via the induction of double strand breaks in genomic DNA. The chapter begins with an overview of the field of gene targeting, and documents the design, synthesis, and testing of a novel method for high efficiency homologous recombination. The method relies on engineering two reagents, a small molecule DNA targeting element and a nuclease fusion protein. The targeting element, a peptide nucleic acid (PNA), was designed and synthesized to target DNA via Watson-Crick base pairing. The PNA also was covalently linked to the small molecule, trimethoprim, to recruit a DHFR nuclease fusion protein to a specific DNA site. Our studies show that the individual interactions of PNA/DNA and of PNA/nuclease can readily occur. Further, the ternary complex of PNA, DNA, and nuclease can form in solution. Chapters Two, Three, and Four describe the development and further characterization of a general method to perturb protein stability and function. Briefly, an unstable protein domain, termed a destabilizing domain (DD), can confer instability to a fused protein of interest and promote its rapid degradation. This instability can be rescued by the addition of a small molecule, Shield-1. Our work in Chapter Two describes the use of this system to regulate protein function in living mammals. In one example, we show that regulation of a secreted protein, the immunomodulatory cytokine IL-2, can control tumor burden in mouse models. Additionally, we used the DD to control the function of TNF-[Alpha] after systemic delivery to a tumor. Chapter Three expands on these in vivo efforts by employing the system to regulate secreted FGF2, an important modulator of bone formation, for skeletal tissue engineering. Shield-1 induction of FGF2 causes induction of bone formation in a calvarial-defect model. Chapter Four presents our observations on the behavior of the DD in various cellular environments --the cytoplasm, nucleus, endoplasmic reticulum, and mitochondria- and in the presence of small molecules that modulate protein production, degradation, and local protein quality control machinery. These data indicate that the levels of the DD, in the presence and absence ligand, is dependent on its subcellular locale and protein homeostasis machinery. The fifth chapter of this dissertation reports the development of a method to assess spatiotemporal cell-cell relationships in real-time and in living animals. The method is based on small-molecule diffusion of an activatable substrate between two populations of cells, thus allowing assessment of cell-cell proximity in vivo via bioluminescence imaging. One cell population catalyzes the release of a caged luciferin. The free luciferin can diffuse to a nearby population of cells expressing luciferase capable of light-emitting catalysis. Thus the luciferase cells in closest proximity to the "pool" of free luciferin emit the most light. We demonstrate the utility of this system in vitro and in vivo and are currently investigating its use for the detection of cancer and early metastatic disease in mouse models.
The Chemistry Of Imaging Probes
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2018
The Chemistry Of Imaging Probes written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
Over the past decades, the field of molecular imaging has been rapidly growing involving multiple disciplines such as medicine, biology, chemistry, pharmacology and biomedical engineering. Any molecular imaging procedure requires an imaging probe that is an agent used to visualize, characterize and quantify biological processes in living systems. Such a probe typically consists of an agent that usually produces signal for imaging purpose, a targeting moiety, and a linker connecting the targeting moiety and the signaling agent. Many challenging problems of molecular imaging can be addressed by exploiting the great possibilities offered by modern synthetic organic and coordination chemistry and the powerful procedures provided by conjugation chemistry. Thus, chemistry plays a decisive role in the development of this cutting-edge methodology. Currently, the diagnostic imaging modalities include Magnetic Resonance Imaging (MRI), Computed Tomography (CT), Ultrasound (US), Nuclear Imaging (PET, SPECT), Optical Imaging (OI) and Photoacoustic Imaging (PAI). Each of these imaging modalities has its own advantages and disadvantages, and therefore, a multimodal approach combining two techniques is often adopted to generate complementary anatomical and functional information of the disease. The basis for designing imaging probes for a given application is dictated by the chosen imaging modality, which in turn is dependent upon the concentration and localization profile (vascular, extracellular matrix, cell membrane, intracellular, near or at the cell nucleus) of the target molecule. The development of high-affinity ligands and their conjugation to the targeting vector is also one of the key steps for pursuing efficient molecular imaging probes. Other excellent reviews, text and monographs describe the principles of biomedical imaging, focusing on molecular biology or on the physics behind the techniques. This Research Topic aims to show how chemistry can offer molecular imaging the opportunity to express all its potential.
Visualizing Chemistry
DOWNLOAD
Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2006-07-01
Visualizing Chemistry written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-07-01 with Science categories.
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.