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Chicken Antibodies

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Chicken Egg Yolk Antibodies Production And Application

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Author : Rüdiger Schade
language : en
Publisher: Springer
Release Date : 2014-11-14

Chicken Egg Yolk Antibodies Production And Application written by Rüdiger Schade and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-14 with Science categories.

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs. Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997.

Chicken Antibodies

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Author : Devisri Vyshnavi Yarlagadda
language : en
Publisher: Lulu.com
Release Date :

Chicken Antibodies written by Devisri Vyshnavi Yarlagadda and has been published by Lulu.com this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.

Chicken Antibodies

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Author : Gordon Ronald Dreesman
language : en
Publisher:
Release Date : 1966

Chicken Antibodies written by Gordon Ronald Dreesman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1966 with Antigens and antibodies categories.

Production Application And Stability Of Chicken Egg Yolk Antibody Lgy

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Author : Xiujie Li
language : en
Publisher: National Library of Canada = Bibliothèque nationale du Canada
Release Date : 2000

Production Application And Stability Of Chicken Egg Yolk Antibody Lgy written by Xiujie Li and has been published by National Library of Canada = Bibliothèque nationale du Canada this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Antigens categories.

Engineering Recombinant Chicken Antibodies For Improved Characteristics

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Author : Joy Sixholo
language : en
Publisher:
Release Date : 2008

Engineering Recombinant Chicken Antibodies For Improved Characteristics written by Joy Sixholo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Bacteriophages categories.

Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can be engineered to improve their binding or other characteristics. A chicken single chain variable fragment (scFv) phage library was panned against the 16 kDa antigen of Mycobacterium tuberculosis. Three phage displayed antibodies were obtained which bound specifically to the antigen. In soluble scFv format, however, they produced low ELISA signals. For this reason they were able to be used as models for antibody engineering. Three mutant sub-libraries were created by random mutagenesis. High stringency panning of the mutant sub-libraries against the target antigen yielded stronger binders which produced ELISA signals of up to eleven times higher than the parent scFvs. An increase in affinity was confirmed by surface plasmon resonance. One mutant scFv with a single amino acid exchange also showed an increase in the yield of scFvs it produced. Upon shortening the linker sequence between the heavy and light chains, size exclusion chromatography showed that multimerisation had occurred. Dimers, trimers and tetramers were formed thus increasing the avidity of the scFvs. Tetramers derived from the unmutated scFv showed the greatest improvement in ELISA binding. To improve expression and purification, an alternate bacterial expression vector with a histidine tag was investigated. For this series of experiments the coding region for a chicken scFv directed against VP7 of bluetongue virus was transferred from the pHEN1 display vector to the pSANG 14-3F vector, which fuses the scFv gene to a bacterial alkaline phosphatase gene. A bi-functional chicken scFv-alkaline phosphatase fusion protein that exhibits both alkaline phosphatase activity and specific antigen binding was expressed in Escherichia coli and purified in a single step via metal affinity chromatography. Furthermore the scFv-AP fusion protein was directly detected in ELISA without the use of secondary detection reagents. This study confirms that the strategies used can efficiently enhance the characteristics of chicken scFvs.

Igy Technology Production And Application Of Egg Yolk Antibodies

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Author : Xiao-Ying Zhang
language : en
Publisher: Springer Nature
Release Date : 2021-06-24

Igy Technology Production And Application Of Egg Yolk Antibodies written by Xiao-Ying Zhang and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-24 with Medical categories.

This first edited Volume on IgY-Technology, addresses the historical and dynamic development of IgY-applications. The authors cover the biological basis and theoretical context, methodological guidance, and applications of IgY-Technology. A focus is laid on the use of IgY-antibodies for prophylactic/therapeutic purposes in human and veterinary medicine. Aside from applications, the chapters also offer an evolutionary understanding of the IgY molecule, IgY receptors and practical prerequisites to produce IgY-antibodies. Guidance is given for every step of the process. Starting with an introduction to hens as a model species and including hen husbandry, hen egg-laying capacity and total IgY outcomes. Readers will also learn about immunization techniques, the advantages and limitations of different IgY extraction methods, as well as storage stability of the final product. The last part of the volume highlights hands-on aspects of applications, such as IgY delivery strategies, new methods to produce monoclonal IgY-antibodies or production of functional IgY fragments by phage-display as well as commercial exploitation of the technology. Thus, this book is a valuable resource and guide for Scientists, Clinicians and Health Product Developers in both human and veterinary medicine.