Confocal Laser Scanning Microscopy Of Living Cells
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Confocal Laser Scanning Microscopy Of Living Cells
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Author : Alexey Moshkov
language : en
Publisher:
Release Date : 2020
Confocal Laser Scanning Microscopy Of Living Cells written by Alexey Moshkov and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Electronic books categories.
Living cells are distinguished in their ability to keep the structural stability in the changing environment by means of energy supply. Understanding the mechanisms of energy conversion is impossible without knowledge of the physiological processes involved. This question is addressed to confocal laser scanning microscopy (CLSM) that has become a leader among the group of light microscopy method. The high resolution in CLSM images is reached without deconvolution procedures. Linear characteristics of its variable parameters are important for quantitative image analysis. These properties of CLSM are indispensable in the study of mitochondrial membrane potential (MMP) using fluorescent dyes. Lipophilic rhodamine dyes freely pass the plasma membrane and accumulate in the mitochondria according to electrical potential difference. CLSM images of TMRE (tetramethylrhodamine, ethyl ester)-stained U937 cells were analyzed by standard image processing procedures in ImageJ software. These procedures allow the creation of mask, applying it to original image. Average fluorescence intensity in selected regions was used as a measure of MMP changes during phytohemagglutinin treatment. This value was decreased by 34% after 2 h of lectin treatment. Some cells after mitogenic stimulation completely lost MMP. Deregulation of mitochondrial calcium handling and changes of cytoplasmic monovalent ion concentration are considered as mechanisms of MMP decrease during lymphocyte stimulation.
Confocal Microscopy And Multiphoton Excitation Microscopy
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Author : Barry R. Masters
language : en
Publisher: SPIE Press
Release Date : 2006
Confocal Microscopy And Multiphoton Excitation Microscopy written by Barry R. Masters and has been published by SPIE Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Confocal microscopy categories.
This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.
Three Dimensional Confocal Microscopy Volume Investigation Of Biological Specimens
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Three Dimensional Confocal Microscopy Volume Investigation Of Biological Specimens written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color
Confocal Microscopy
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Author : Stephen W. Paddock
language : en
Publisher: Humana Press
Release Date : 2013-09-20
Confocal Microscopy written by Stephen W. Paddock and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09-20 with Medical categories.
Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.
Cell Biological Applications Of Confocal Microscopy
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Author :
language : en
Publisher: Academic Press
Release Date : 2003-01-03
Cell Biological Applications Of Confocal Microscopy written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-01-03 with Science categories.
This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures
Optical Microscopy
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Author : Brian Herman
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Optical Microscopy written by Brian Herman and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances Caged compounds, fluorescence ratio imaging, and CCD video cameras Simultaneous multiple detection and real-time fluorescence microscopy Simultaneous DIC and quantitative LLF video imaging Total internal reflectance, time-resolved, and automated fluorescence microscopy Laser-scanning confocal microscopy Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells
Confocal And Two Photon Microscopy
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Author : Alberto Diaspro
language : en
Publisher: John Wiley & Sons
Release Date : 2001-11-22
Confocal And Two Photon Microscopy written by Alberto Diaspro and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-11-22 with Science categories.
Confocal and Two-Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy. Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring: * In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications * Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules * Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references * Companion FTP site with full-color photographs * The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.
Confocal Microscopy For Biologists
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Author : Alan R. Hibbs
language : en
Publisher: Springer Science & Business Media
Release Date : 2004-04-30
Confocal Microscopy For Biologists written by Alan R. Hibbs and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-04-30 with Science categories.
There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.
Fluorescence Methods For Investigation Of Living Cells And Microorganisms
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Author : Natalia Grigoryeva
language : en
Publisher: BoD – Books on Demand
Release Date : 2020-09
Fluorescence Methods For Investigation Of Living Cells And Microorganisms written by Natalia Grigoryeva and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09 with Cytofluorometry categories.
Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
Fluorescence Microscopy Of Living Cells In Culture Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 1989-04-01
Fluorescence Microscopy Of Living Cells In Culture Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-04-01 with Science categories.
Fluorescence Microscopy of Living Cells in Culture, Part B