Fluorescence Methods For Investigation Of Living Cells And Microorganisms
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Fluorescence Methods For Investigation Of Living Cells And Microorganisms
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Author : Natalia Grigoryeva
language : en
Publisher: BoD – Books on Demand
Release Date : 2020-09
Fluorescence Methods For Investigation Of Living Cells And Microorganisms written by Natalia Grigoryeva and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09 with Medical categories.
Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
New Trends In Fluorescence Spectroscopy
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Author : Bernard Valeur
language : en
Publisher: Springer Science & Business Media
Release Date : 2001
New Trends In Fluorescence Spectroscopy written by Bernard Valeur and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Medical categories.
This first volume in the new Springer Series on Fluorescencebrings together fundamental and applied research from this highly interdisciplinary field ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of state-of-the-art articles will serve as an invaluable tool for practitioners and novices and provide inspiration for new developments and applications.
Live Cell Imaging
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Author : Robert D. Goldman
language : en
Publisher: CSHL Press
Release Date : 2005
Live Cell Imaging written by Robert D. Goldman and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Science categories.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Patch Clamp Technique Current Methods And Future Prospects
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Author :
language : en
Publisher: BoD – Books on Demand
Release Date : 2024-12-11
Patch Clamp Technique Current Methods And Future Prospects written by and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-12-11 with Science categories.
This book provides a comprehensive guide to both established and innovative methodologies for exploring ion channel function across various applications. Each chapter begins with a helpful introduction to orient nonexpert readers, providing background and context for the methods discussed, followed by detailed, step-by-step protocols for practical implementation. Topics covered include techniques such as macropatch recordings, bilayer recordings, dynamic clamp, organotypic slice culture, as well as advanced approaches like combined in vivo patch-clamp recording with optogenetics and multielectrode array technology.
Biosensing Technologies For The Detection Of Pathogens
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Author : Toonika Rinken
language : en
Publisher: BoD – Books on Demand
Release Date : 2018-03-21
Biosensing Technologies For The Detection Of Pathogens written by Toonika Rinken and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-21 with Medical categories.
Rapid multiplex detection of pathogens in the environment and in our food is a key factor for the prevention and effective treatment of infectious diseases. Biosensing technologies combining the high selectivity of biomolecular recognition and the sensitivity of modern signal detection platforms are a prospective option for automated analyses. They allow rapid detection of single molecules as well as cellular substances. This book, including 12 chapters from 50 authors, introduces the principles of identification of specific pathogen biomarkers along with different biosensor-based technologies applied for pathogen detection.
Biomolecular Interactions Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2021-10-29
Biomolecular Interactions Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-29 with Science categories.
Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions
Algal Biotechnology
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Author : Ashfaq Ahmad
language : en
Publisher: Elsevier
Release Date : 2022-04-26
Algal Biotechnology written by Ashfaq Ahmad and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-26 with Science categories.
Algae Biotechnology: Integrated Algal Engineering for Bioenergy, Bioremediation, and Biomedical Applications covers key applications of algae for bioenergy and how to integrate the production of biofuels with environmental, nutraceutical and biomedical processes and products. The book emphasizes cost-effective biofuels production through integrated biorefinery, combining continuous processes and various algae as feedstock to produce biofuel, bioenergy and various high value biochemicals. Novel algal culturing technologies and bioprocess engineering techniques are provided for the optimization of operational approaches for commercial-scale production, as well as to reduce the overall costs. New and existing molecular methods for genetic and metabolic engineering of algae are also presented. Furthermore, methods for the optimization of existing biochemical pathways are explained, and new pathways are introduced, in order to maximize the potential for biofuels production and related nutraceutical and biomedical co-products. This book provides an ideal roadmap for bioenergy researchers and engineers who want to incorporate valuable nutraceutical and biomedical products and environmental practices into the production of biofuels. - Addresses issues faced by the bioenergy sector and how to resolve them through the integration of algal biotechnology and engineering - Provides a guide to the efficient and cost-effective production of bioenergy, while simultaneously mitigating pollution and producing valuable nutraceutical and biomedical biproducts - Covers new and emerging approaches in integrated algal biotechnology - Offers a roadmap to their application in the production of biofuels alongside nutraceutical, biomedical, and environmental processes and products
Microalgal Biotechnology
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Author : Eduardo Jacob-Lopes
language : en
Publisher: BoD – Books on Demand
Release Date : 2018-06-27
Microalgal Biotechnology written by Eduardo Jacob-Lopes and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-27 with Medical categories.
Microalgal Biotechnology presents an authoritative and comprehensive overview of the microalgae-based processes and products. Divided into 10 discreet chapters, the book covers topics on applied technology of microalgae. Microalgal Biotechnology provides an insight into future developments in each field and extensive bibliography. It will be an essential resource for researchers and academic and industry professionals in the microalgae biotechnology field.
Handbook Of Single Molecule Biophysics
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Author : Peter Hinterdorfer
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-12-24
Handbook Of Single Molecule Biophysics written by Peter Hinterdorfer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-12-24 with Science categories.
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Introduction To Fluorescence
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Author : David M. Jameson
language : en
Publisher: CRC Press
Release Date : 2025-03-26
Introduction To Fluorescence written by David M. Jameson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-26 with Science categories.
The phenomenon known as ‘fluorescence’ is now widely used in the chemical, physical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. This Second Edition of Introduction to Fluorescence helps readers acquire a thorough understanding of basic fluorescence theory and practice. It describes the general principles in a direct way and uses examples from a variety of disciplines to demonstrate them. Since the First Edition, significant advances in the field have appeared. For example, phasors, both lifetime and spectral phasors, have become very popular, and so a new chapter dedicated to this topic has been added in this edition. Furthermore, significant advances have been made in fluorescence microscopy methods, including super-resolution and single-molecule techniques. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Numerous references are given in each chapter, along with a list of particularly important references at the end of each chapter. The text incorporates more than 340 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, Chapter 13, the Appendix, provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.