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Handbook Of Single Molecule Biophysics


Handbook Of Single Molecule Biophysics
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Handbook Of Single Molecule Biophysics


Handbook Of Single Molecule Biophysics
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Author : Peter Hinterdorfer
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-12-24

Handbook Of Single Molecule Biophysics written by Peter Hinterdorfer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-12-24 with Science categories.


This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.



An Introduction To Single Molecule Biophysics


An Introduction To Single Molecule Biophysics
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Author : Yuri L. Lyubchenko
language : en
Publisher: CRC Press
Release Date : 2017-11-22

An Introduction To Single Molecule Biophysics written by Yuri L. Lyubchenko and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11-22 with Science categories.


This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.



Handbook Of Single Molecule Fluorescence Spectroscopy


Handbook Of Single Molecule Fluorescence Spectroscopy
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Author : Chris Gell
language : en
Publisher: OUP Oxford
Release Date : 2006-08-17

Handbook Of Single Molecule Fluorescence Spectroscopy written by Chris Gell and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-08-17 with Science categories.


Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.



Theory And Evaluation Of Single Molecule Signals


Theory And Evaluation Of Single Molecule Signals
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Author : Eli Barkai
language : en
Publisher: World Scientific
Release Date : 2008

Theory And Evaluation Of Single Molecule Signals written by Eli Barkai and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.


This book reviews recently developed theoretical and numerical approaches to deal with optical and mechanical signals from individual molecules. The character of data generated by single molecules, and more generally by single nano-objects, qualitatively differs from those obtained in conventional experiments on large ensembles of molecules. Fluctuations, randomness and irreproducibility are central to single-molecule measurements, and the specific methods required to extract reliable and statistically relevant information from them are presented here. With contributions mainly from participants of the OC Theory, Modeling and Evaluation of Single-Molecule MeasurementsOCO workshop held in Leiden, the Netherlands, on April 16-20, 2007, this book is an authoritative compendium on the subject.



Handbook Of Fluorescence Spectroscopy And Imaging


Handbook Of Fluorescence Spectroscopy And Imaging
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Author : Markus Sauer
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-23

Handbook Of Fluorescence Spectroscopy And Imaging written by Markus Sauer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-23 with Science categories.


Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.



Single Molecule Biophysics And Poisson Process Approach To Statistical Mechanics


Single Molecule Biophysics And Poisson Process Approach To Statistical Mechanics
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Author : Susanta K Sarkar
language : en
Publisher: Morgan & Claypool Publishers
Release Date : 2016-04-01

Single Molecule Biophysics And Poisson Process Approach To Statistical Mechanics written by Susanta K Sarkar and has been published by Morgan & Claypool Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-01 with Science categories.


This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.



Handbook Of Dynein Second Edition


Handbook Of Dynein Second Edition
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Author : Keiko Hirose
language : en
Publisher: CRC Press
Release Date : 2019-05-28

Handbook Of Dynein Second Edition written by Keiko Hirose and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-28 with Science categories.


Dyneins are molecular motors that are involved in various cellular processes, such as cilia and flagella motility, vesicular transport, and mitosis. Since the first edition of this book was published in 2012, there has been a significant breakthrough: the crystal structures of the motor domains of cytoplasmic dynein have been solved and the previously unknown details of this huge and complex molecule have been unveiled. This new edition contains 14 chapters written by researchers in the US, Europe, and Asia, including 3 new chapters that incorporate new fields. The other chapters have also been substantially updated. Compared with the earlier edition, this book focuses more on the motile mechanisms of dynein, especially by biophysical methods such as cryo-EM, X-ray crystallography, and single-molecule nanometry. It is a major handbook for frontline researchers as well as for advanced students studying cell biology, molecular biology, biochemistry, biophysics, and structural biology.



Biomolecular Kinetics


Biomolecular Kinetics
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Author : Clive R. Bagshaw
language : en
Publisher: CRC Press
Release Date : 2017-10-04

Biomolecular Kinetics written by Clive R. Bagshaw and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-04 with Medical categories.


"a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide" –Mark Leake, University of York "destined to become a standard reference.... Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice." –Michael Geeves, University of Kent "covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" –Stephen Halford, University of Bristol This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A.



Single Molecule Science


Single Molecule Science
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Author : Krishnarao Appasani
language : en
Publisher:
Release Date : 2022-05-11

Single Molecule Science written by Krishnarao Appasani and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-11 with Medical categories.


A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.



Advances In Chemical Physics Volume 162


Advances In Chemical Physics Volume 162
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Author : Stuart A. Rice
language : en
Publisher: John Wiley & Sons
Release Date : 2017-10-09

Advances In Chemical Physics Volume 162 written by Stuart A. Rice and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-09 with Science categories.


The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. • This is the only series of volumes available that presents the cutting edge of research in chemical physics. • Includes contributions from experts in this field of research. • Contains a representative cross-section of research that questions established thinking on chemical solutions • Structured with an editorial framework that makes the book an excellent supplement to an advanced graduate class in physical chemistry or chemical physics