Patch Clamp Technique Current Methods And Future Prospects
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Patch Clamp Technique Current Methods And Future Prospects
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Author :
language : en
Publisher: BoD – Books on Demand
Release Date : 2024-12-11
Patch Clamp Technique Current Methods And Future Prospects written by and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-12-11 with Science categories.
This book provides a comprehensive guide to both established and innovative methodologies for exploring ion channel function across various applications. Each chapter begins with a helpful introduction to orient nonexpert readers, providing background and context for the methods discussed, followed by detailed, step-by-step protocols for practical implementation. Topics covered include techniques such as macropatch recordings, bilayer recordings, dynamic clamp, organotypic slice culture, as well as advanced approaches like combined in vivo patch-clamp recording with optogenetics and multielectrode array technology.
Mastering The Patch Clamp Technique A Practical Guide
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Author : Fabian Christoph Roth
language : en
Publisher: Springer Nature
Release Date : 2025-04-16
Mastering The Patch Clamp Technique A Practical Guide written by Fabian Christoph Roth and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-04-16 with Science categories.
The patch clamp technique is one of the most important electrophysiological methods in use today. Its application in basic biomedical research has provided significant insights into the function and properties of ion channels, making it equally indispensable in applied pharmacological research. This book is the English edition of the popular German introduction to the patch clamp technique. This introductory book serves as a practical guide for teachers and students, offering clear and practical instructions on patch clamp recordings. The authors provide essential information to establish and successfully apply this method in the laboratory. The book covers the basics of measurements, the detailed setup of a patch clamp rig and how to carry out typical recordings. It also includes concrete tips and tricks to help with troubleshooting, making it an indispensable resource. The book not only highlights the enormous potential of the method for the study of ion channels and cell signaling, but also provides an introduction to the major developments and variations of the technique. The book is aimed at advanced students of biology, biochemistry, medicine, pharmacy and related disciplines as well as scientists who want to better understand and/or acquire the technique.
Patch Clamp Analysis
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Author : Wolfgang Walz
language : en
Publisher: Humana
Release Date : 2007-08-02
Patch Clamp Analysis written by Wolfgang Walz and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-02 with Medical categories.
Continuing the research of the best-selling first edition, this second edition collects three more years of research in the ever-expanding study of the cell membrane. It covers the latest developments in the "traditional" patch techniques. This authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field. Thorough and timely, this edition is an invaluable resource.
Patch Clamp Techniques
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Author : Yasunobu Okada
language : en
Publisher: Springer
Release Date : 2012-04-27
Patch Clamp Techniques written by Yasunobu Okada and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-27 with Medical categories.
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Dynamic Clamp
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Author : Alain Destexhe
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-03-11
Dynamic Clamp written by Alain Destexhe and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-11 with Medical categories.
Dynamic-clamp is a fascinating electrophysiology technique that consists of merging living neurons with computational models. The dynamic-clamp (also called “conductance injection”) allows experimentalists and theoreticians to challenge neurons (or any other type of cell) with complex conductance stimuli generated by a computer. The technique can be implemented from neural simulation environments and a variety of custom-made or commercial systems. The real-time interaction between the computer and cell also enables the design of recording paradigms with unprecedented accuracy via a computational model of the electrode. Dynamic-Clamp: From Principles to Applications contains contributions from leading researchers in the field, who investigate these paradigms at the cellular or network level, in vivo and in vitro, and in different brain regions and cardiac cells. Topics discussed include the addition of artificially-generated synaptic activity to neurons; adding, amplifying or neutralizing voltage-dependent conductances; creating hybrid networks with real and artificial cells; attaching simulated dendritic tree structures to the living cell; and connecting different neurons. This book will be of interest to experimental biophysicists, neurophysiologists, and cardiac physiologists, as well as theoreticians, engineers, and computational neuroscientists. Graduate and undergraduate students will also find up-to-date coverage of physiological problems and how they are investigated.
Protein Engineering
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Author : Mallorie N. Sheehan
language : en
Publisher: Nova Science Publishers
Release Date : 2013-07
Protein Engineering written by Mallorie N. Sheehan and has been published by Nova Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07 with Protein engineering categories.
Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin.
Wheat
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Author : Y. P. S. Bajaj
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Wheat written by Y. P. S. Bajaj and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Technology & Engineering categories.
Wheat, which is the second most important cereal crop in the world, is being grown in a wide range of climates over an area of about 228 945 thou sand ha with a production of about 535 842 MT in the world. Bread wheat (Triticum aestivum L. ) accounts for 80% of the wheat consumption, howe ver, it is attacked by a large number of pests and pathogens; rusts and smuts cause enormous damage to the crop and reduce the yield drastically in some areas. The major breeding objectives for wheat include grain yield, earliness, resistance to lodging and diseases, spikelet fertility, cold tolerance, leaf duration and net assimilation rate, fertilizer utilization, coleoptile length, nutritional value, organoleptic qualities, and the improvement of charac ters such as color and milling yield. The breeding of wheat by traditional methods has been practiced for centuries, however, it has only now come to a stage where these methods are insufficient to make any further breakthrough or to cope with the world's demand. Although numerous varieties are released every year around the world, they do not last long, and long-term objectives cannot be realized unless more genetic variability is generated. Moreover, the intro duction of exotic genetic stocks and their cultivation over large areas results in the depletion and loss of the native germplasm pool.
Intelligent Nanosystems For Energy Information And Biological Technologies
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Author : Jun’ichi Sone
language : en
Publisher: Springer
Release Date : 2016-09-17
Intelligent Nanosystems For Energy Information And Biological Technologies written by Jun’ichi Sone and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-17 with Technology & Engineering categories.
This book gives a state-of-the-art view by recognized researchers of the nanotechnologies required for future integrated systems leading to innovations in energy, the environment, and biotechnologies. Nanostructures that would be difficult to form using the current semiconductor technology will be realized using a combination of bottom-up and top-down processes, including hybrid nanostructures made of inorganic and organic/biological materials. Bio-sensing, imaging, and cell or molecular manipulation are discussed in Chapters 2–7. The acquisition of basic knowledge on the cellular level will lead to curing serious diseases. Also, nanofabrication technologies, discussed in Chapters 8–15, will lead to next-generation solar cells, secondary batteries, and advanced electronic circuits using nanostructured materials, thus providing solutions for serious energy and environment issues. Prospective readers of this book include graduate students as well as researchers and engineers working in this field.
Patch Clamp Analysis
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Author : Wolfgang Walz
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-06
Patch Clamp Analysis written by Wolfgang Walz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-06 with Medical categories.
Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
Patch Clamp Electrophysiology
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Author : Mark Dallas
language : en
Publisher: Humana
Release Date : 2020-10-30
Patch Clamp Electrophysiology written by Mark Dallas and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-30 with Medical categories.
This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.