Mastering The Patch Clamp Technique A Practical Guide
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Mastering The Patch Clamp Technique A Practical Guide
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Author : Fabian Christoph Roth
language : en
Publisher: Springer Nature
Release Date : 2025-04-16
Mastering The Patch Clamp Technique A Practical Guide written by Fabian Christoph Roth and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-04-16 with Science categories.
The patch clamp technique is one of the most important electrophysiological methods in use today. Its application in basic biomedical research has provided significant insights into the function and properties of ion channels, making it equally indispensable in applied pharmacological research. This book is the English edition of the popular German introduction to the patch clamp technique. This introductory book serves as a practical guide for teachers and students, offering clear and practical instructions on patch clamp recordings. The authors provide essential information to establish and successfully apply this method in the laboratory. The book covers the basics of measurements, the detailed setup of a patch clamp rig and how to carry out typical recordings. It also includes concrete tips and tricks to help with troubleshooting, making it an indispensable resource. The book not only highlights the enormous potential of the method for the study of ion channels and cell signaling, but also provides an introduction to the major developments and variations of the technique. The book is aimed at advanced students of biology, biochemistry, medicine, pharmacy and related disciplines as well as scientists who want to better understand and/or acquire the technique.
Incretin Biology A Practical Guide
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Author : G. U. Y. A. RUTTER
language : en
Publisher: World Scientific
Release Date : 2015-10-22
Incretin Biology A Practical Guide written by G. U. Y. A. RUTTER and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-10-22 with Medical categories.
Together, obesity and diabetes form a 21st century epidemic, the treatment of which consumes a substantial fraction of global health costs. New and improved therapies are therefore desperately needed.Incretins are short peptide hormones released from the gut in response to food transit. These hormones regulate a plethora of processes to control food intake, body weight and glucose homeostasis. This is one of the fastest areas of growth within diabetes research, and over the past decade, increasing numbers of novel diabetes drugs -- many based on incretin action -- have been added to the therapeutic armoury.Following an introduction detailing the historical perspective and discovery of incretin therapies and clinical applications, the book looks at the release of incretins at a cellular level from entero-endocrine cells, and actions on the pancreas (alpha and beta cells) and brain. It then looks at changes in incretins seen in diabetes and following bariatric surgery. Finally, the side effects of incretin therapy are discussed. Importantly, each chapter contains a methodology section aimed at providing a practical guide to key techniques in the field of incretin biology and ranges from basic laboratory research to clinical practice.Incretin Biology -- A Practical Guide offers a unique perspective within this field of research by bringing together scientists, physicians and bariatric surgeons. Thus, it is a comprehensive text that offers a broad overview of the subject.
Fluorescent And Luminescent Probes For Biological Activity
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Author : W. T. Mason
language : en
Publisher: Elsevier
Release Date : 1999-04-16
Fluorescent And Luminescent Probes For Biological Activity written by W. T. Mason and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-04-16 with Science categories.
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.TOPICS COVERED:* Single and multiphoton confocal microscopy* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies* Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells* Use of optical probes to detect microorganisms* Raman and confocal raman microspectroscopy* Fluorescence lifetime imaging microscopy* Digital CCD cameras and their application in biological microscopy
Signal Transduction Single Cell Techniques
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Author : Bert, van Duijn
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Signal Transduction Single Cell Techniques written by Bert, van Duijn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.
Patch Clamping
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Author : Areles Molleman
language : en
Publisher: John Wiley & Sons
Release Date : 2003-06-13
Patch Clamping written by Areles Molleman and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-06-13 with Science categories.
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
The Laboratory Computer
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Author : John Dempster
language : en
Publisher: Elsevier
Release Date : 2001-07-02
The Laboratory Computer written by John Dempster and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-07-02 with Computers categories.
The Laboratory Computer: A Practical Guide for Physiologists and Neuroscientists introduces the reader to both the basic principles and the actual practice of recording physiological signals using the computer. It describes the basic operation of the computer, the types of transducers used to measure physical quantities such as temperature and pressure, how these signals are amplified and converted into digital form, and the mathematical analysis techniques that can then be applied. It is aimed at the physiologist or neuroscientist using modern computer data acquisition systems in the laboratory, providing both an understanding of how such systems work and a guide to their purchase and implementation. - The key facts and concepts that are vital for the effective use of computer data acquisition systems - A unique overview of the commonly available laboratory hardware and software, including both commercial and free software - A practical guide to designing one's own or choosing commercial data acquisition hardware and software
Doody S Rating Service
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Author : Daniel J. Doody
language : en
Publisher:
Release Date : 1996
Doody S Rating Service written by Daniel J. Doody and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Health & Fitness categories.
Guide To Research Techniques In Neuroscience
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Author : Matt Carter
language : en
Publisher: Academic Press
Release Date : 2022-03-26
Guide To Research Techniques In Neuroscience written by Matt Carter and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-26 with Medical categories.
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step
Patch Clamp Techniques
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Author : Yasunobu Okada
language : en
Publisher: Springer
Release Date : 2012-04-27
Patch Clamp Techniques written by Yasunobu Okada and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-27 with Medical categories.
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Opioid Research
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Author : Zhizhong Z. Pan
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Opioid Research written by Zhizhong Z. Pan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Medical categories.
Opioid research is one of the multidisciplinary research areas that involve advanced techniques ranging from molecular genetics to neuropharmacology, and from behavioral neuroscience to clinical medicine. In current opioid research, it has become increasingly important to use multiple approaches at molecular, cellular, and system levels for investigations on a specific opio- related target system. That often requires understanding and applying cro- field techniques and methods for the success of one’s research projects. Through its broad spectrum of coverage, Opioid Research: Methods and Protocols provides a comprehensive collection of major laboratory methods and protocols in current opioid research, covering topics from molecular and genetic techniques to behavioral analyses of animal models, and then to clinical practice. It will serve as a convenient reference book from which those involved in opioid research will learn or perfect the necessary cross-field techniques. The detailed methods and protocols described in Opioid Research: Methods and Protocols have each been successfully applied in current opioid research. Part I provides molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways. Part II includes primary techniques for mapping the distributions and detecting the expression levels of opioid receptors, opioid peptides, and their messages in brain tissues and in individual cells. Part III deals with methods for creating in vitro receptor models and in vivo animal models to study opioid functions. Part IV describes practical applications of opioids in clinical medicine for the treatment of pain and opioid addiction.