Fluorescent And Luminescent Probes For Biological Activity
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Fluorescent And Luminescent Probes For Biological Activity
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Author : W. T. Mason
language : en
Publisher: Elsevier
Release Date : 1999-04-16
Fluorescent And Luminescent Probes For Biological Activity written by W. T. Mason and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-04-16 with Science categories.
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.TOPICS COVERED:* Single and multiphoton confocal microscopy* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies* Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells* Use of optical probes to detect microorganisms* Raman and confocal raman microspectroscopy* Fluorescence lifetime imaging microscopy* Digital CCD cameras and their application in biological microscopy
Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Fluorescence Spectroscopy And Microscopy In Biology
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Author : Radek Šachl
language : en
Publisher: Springer Nature
Release Date : 2023-04-27
Fluorescence Spectroscopy And Microscopy In Biology written by Radek Šachl and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-27 with Science categories.
This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
Fluorescence Spectroscopy Imaging And Probes
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Author : Ruud Kraayenhof
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Fluorescence Spectroscopy Imaging And Probes written by Ruud Kraayenhof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.
Handbook Of Biological Dyes And Stains
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Author : R. W. Sabnis
language : en
Publisher: John Wiley & Sons
Release Date : 2010-03-29
Handbook Of Biological Dyes And Stains written by R. W. Sabnis and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-29 with Science categories.
A COMPLETE, UP-TO-DATE RESOURCE OF INFORMATION ON MORE THAN 200 DYES AND STAINS Handbook of Biological Dyes and Stains is the most comprehensive volume available on the subject, covering all the available dyes and stains known to date in the literature for use in biology and medicine. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, and related fields. This is the first book to give the CAS registry number, chemical structure, Chemical Abstracts index name, all other chemical names, Merck Index number, chemical/dye class, molecular formula, molecular weight, physical form, solubility, melting point, boiling point, pH range, color change at pH, pKa, absorption, and emission maxima of dyes and stains, as well as to provide access to synthesis procedures (lab scale and industrial scale) of dyes and stains. This user-friendly handbook also features references on safety, toxicity, and adverse effects of dyes and stains on humans, animals, and the environment, including: acute/chronic toxicity aquatic toxicity carcinogenicity cytotoxicity ecotoxicity genotoxicity hepatotoxicity marine toxicity mutagenicity nephrotoxicity neurotoxicity oral toxicity phototoxicity phytotoxicity The use of biological dyes and stains has extremely high potential in today's business environment. This makes Handbook of Biological Dyes and Stains a convenient, must-have reference. Its staining, biological, and industrial applications make it a vital resource for industrial and academic researchers; the book also serves as a valuable desktop reference for medical professionals, biologists, chemists, chemical/optical engineers, physicists, materials scientists, intellectual property professionals, students, and professors.
Handbook Of Biomedical Fluorescence
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Author : Mary-Ann Mycek
language : en
Publisher: CRC Press
Release Date : 2019-07-17
Handbook Of Biomedical Fluorescence written by Mary-Ann Mycek and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-17 with Medical categories.
Melding basic and clinical science, this reference provides a comprehensive overview of the roles that biophysics, photochemistry, and computational modeling play in the biomedical applications of fluorescence spectroscopy and imaging. Penned by pioneering researchers, the Handbook of Biomedical Fluorescence discusses fundamental aspects of fluorescence generation in organic molecules within tissue, theoretical and experimental views of how light propagation in tissue can be used to interpret fluorescence signals, endogenous and exogenous fluorescence agents in medical or basic research studies, and radiation transport, diffusion theory, and the Monte Carlo method.
Measurement And Manipulation Of Intracellular Ions
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Author :
language : en
Publisher: Academic Press
Release Date : 1995-10-10
Measurement And Manipulation Of Intracellular Ions written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-10-10 with Science categories.
This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures. - A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies - Includes overviews of Optical probes and reagents - Fabrication and use of ion-selective microelectrodes - Use of NMR spectroscopy - Ionophores
Introduction To Experimental Biophysics
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Author : Jay L. Nadeau
language : en
Publisher: CRC Press
Release Date : 2017-10-10
Introduction To Experimental Biophysics written by Jay L. Nadeau and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-10 with Medical categories.
Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.
Lasers And Current Optical Techniques In Biology
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Author : Giuseppe Palumbo
language : en
Publisher: Royal Society of Chemistry
Release Date : 2004
Lasers And Current Optical Techniques In Biology written by Giuseppe Palumbo and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.
The wide range of topics covered make this book of interest to a diverse range of scientific communities.
The Apoplast Of Higher Plants Compartment Of Storage Transport And Reactions
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Author : Burkhard Sattelmacher
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-06-17
The Apoplast Of Higher Plants Compartment Of Storage Transport And Reactions written by Burkhard Sattelmacher and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-17 with Science categories.
This book summarizes the experimental work conducted during a trans-disciplinary research program conducted for six years by the German Research Foundation. Each chapter includes introductory remarks written by internationally recognized scientists in their research areas. Contributiing authors representing outstanding German scientists from such different disciplines as Physics, Biochemistry, Plant Nutrition, Botany, and Molecular Biology not only report original research but also review the state of knowledge in their fields of research.