Fluorescent And Luminescent Probes For Biological Activity
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Fluorescent And Luminescent Probes For Biological Activity
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Author : W. T. Mason
language : en
Publisher: Elsevier
Release Date : 1999-04-16
Fluorescent And Luminescent Probes For Biological Activity written by W. T. Mason and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-04-16 with Science categories.
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy
Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Optical Probes In Biology
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Author : Jin Zhang
language : en
Publisher: CRC Press
Release Date : 2016-04-19
Optical Probes In Biology written by Jin Zhang and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.
Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone modifications. It critically reviews the different probe designs and delves into the strategies for developing new fluorescent protein varieties with enhanced capabilities. It also covers sophisticated imaging techniques and equipment, such as intensity and lifetime-based fluorescence microscopy methods, used to visualize and track optical probes. In addition, the book goes beyond live-cell tracking to discuss the growing application of activity-based probes for performing pharmacological drug screening and probing molecular processes in living animals. It also discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers. With contributions from top international scientists, this book offers a thorough overview of the latest optical probes in cell biology and biochemistry. Both newcomers and established researchers will discover how to incorporate state-of-the-art optical probes and fluorescence imaging into their research.
Fluorescence Microscopy In Life Sciences
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Author : Juan Carlos Stockert
language : en
Publisher: Bentham Science Publishers
Release Date : 2017-12-15
Fluorescence Microscopy In Life Sciences written by Juan Carlos Stockert and has been published by Bentham Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-15 with Science categories.
Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
Fluorescence Spectroscopy Imaging And Probes
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Author : Ruud Kraayenhof
language : en
Publisher: Springer Science & Business Media
Release Date : 2002
Fluorescence Spectroscopy Imaging And Probes written by Ruud Kraayenhof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods Based On Optical Imaging And Fluorescence
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Author :
language : en
Publisher: Academic Press
Release Date : 2020-06-17
Chemical Tools For Imaging Manipulating And Tracking Biological Systems Diverse Methods Based On Optical Imaging And Fluorescence written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-17 with Science categories.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways
The Laboratory Computer
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Author : John Dempster
language : en
Publisher: Elsevier
Release Date : 2001-07-02
The Laboratory Computer written by John Dempster and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-07-02 with Science categories.
The Laboratory Computer: A Practical Guide for Physiologists and Neuroscientists introduces the reader to both the basic principles and the actual practice of recording physiological signals using the computer. It describes the basic operation of the computer, the types of transducers used to measure physical quantities such as temperature and pressure, how these signals are amplified and converted into digital form, and the mathematical analysis techniques that can then be applied. It is aimed at the physiologist or neuroscientist using modern computer data acquisition systems in the laboratory, providing both an understanding of how such systems work and a guide to their purchase and implementation. The key facts and concepts that are vital for the effective use of computer data acquisition systems A unique overview of the commonly available laboratory hardware and software, including both commercial and free software A practical guide to designing one's own or choosing commercial data acquisition hardware and software
Energetics Of Biological Macromolecules Part C
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Author :
language : en
Publisher: Elsevier
Release Date : 2000-08-09
Energetics Of Biological Macromolecules Part C written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-08-09 with Science categories.
Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal is to understand the energetic relationships between macromoleculer structures and biological functions. This volume supplements Volumes 259 and Volume 295 of Methods in Enzymology. Key Features * Probing Stability of Helical Transmembrane Proteins * Energetics of Vinca Alkaloid Interactions with Tubulin * Deriving Complex Ligand Binding Formulas * Mathematical Modeling of Cooperative Interactions in Hemoglobin * Analysis of Interactions of Regulatory Protein TyrR with DNA * Parsing Free Energy of Drug-DNA Interactions * Use of Fluorescence as Thermodynamics Tool
Fluorescence Probes In Oncology
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Author : Elli Kohen
language : en
Publisher: World Scientific
Release Date : 2002-05-20
Fluorescence Probes In Oncology written by Elli Kohen and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-05-20 with Medical categories.
This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book. Contents:The Nature of LightIntroduction to PhotophysicsBasic Approaches to the Experimental Study of Chemical and Biological LuminescenceMethods and InstrumentationNew Methods. Theoretical Basis and Potential Applications to BiologyFluorescent ProbesApplications of Fluorescence Techniques to Study Biological Processes in Normal and Pathological CellsCell Physiopathology Readership: Students and researchers in oncology, the life sciences, biology and optics. Keywords:Chemiluminescence;Bioluminescence;Green Fluorescent Protein (GFP);Microspectrofluorimetry;Fluorescence Resonance Energy Transfer (FRET);Fluorescence Lifetime Imaging (FLIM);Fluorescence In Situ Hybridization (FISH);Multicolor Spectral Karyotyping (SKY);Fourier Interferometric Spectral Imaging;Fluorescence Imaging;Fluorescence Probe;Oncology;Cell Biology;Pathology;Diagnostics
Systematic Evaluation Of The Mouse Eye
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Author : Richard S. Smith
language : en
Publisher: CRC Press
Release Date : 2001-12-20
Systematic Evaluation Of The Mouse Eye written by Richard S. Smith and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-12-20 with Medical categories.
Completion of the first phase of the Human Genome Project has presented scientists with a mountain of new information. The availability of all human genes and their locations is exciting, but their mechanisms of action and interaction with other genes are often unknown. Certain variations in the environment and characteristics of human genes make i